| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,392,090 | G→T | 28.8% | L655M (CTG→ATG) | yjeP ← | predicted mechanosensitive channel |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,392,090 | 0 | G | T | 28.8% | 134.6 / 33.2 | 59 | L655M (CTG→ATG) | yjeP | predicted mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base G (23/19); new base T (8/9); total (31/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01 | |||||||||||
ATCATCATGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCA > W3110S.gb/4392026‑4392156 | atcatcATGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAAt > 1:1554045/1‑71 (MQ=255)atcatcATGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAAt > 1:3215082/1‑71 (MQ=255) tcatcaTGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATc < 1:659270/71‑1 (MQ=255) atcatGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATccc < 1:4561216/71‑1 (MQ=255) atGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGc > 1:4819017/1‑71 (MQ=255) tCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCttt > 1:581360/1‑71 (MQ=255) ccACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCttt < 1:1171679/70‑1 (MQ=255) ccACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCttt < 1:2328590/70‑1 (MQ=255) cacaGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCttttt < 1:1349482/71‑1 (MQ=255) cacaGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCttttt < 1:1821421/71‑1 (MQ=255) atatGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCt < 1:2505386/71‑1 (MQ=255) tatGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCttttt > 1:730067/1‑64 (MQ=255) tatGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCgg < 1:5143324/49‑1 (MQ=255) tatGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCgg < 1:2308284/49‑1 (MQ=255) tggttggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAg > 1:2111545/1‑71 (MQ=255) gttggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAgg > 1:4950680/1‑70 (MQ=255) gttggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAgg < 1:2341953/70‑1 (MQ=255) ttggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGt < 1:1717963/71‑1 (MQ=255) ttggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCGTTTTCAGGCTGAGGGt < 1:1392313/71‑1 (MQ=255) gttgtCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGaccac > 1:2577017/1‑69 (MQ=255) gttgtCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGaccac > 1:2583288/1‑69 (MQ=255) gttgtCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGaccac > 1:4810437/1‑69 (MQ=255) gttgtCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGac > 1:1871693/1‑66 (MQ=255) ttgtCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCttttt > 1:4828265/1‑50 (MQ=255) ttgtCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCttttt > 1:3017079/1‑50 (MQ=255) ttgtCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGac > 1:260629/1‑65 (MQ=255) ttgtCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGc > 1:1062857/1‑71 (MQ=255) ttgtCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGc > 1:2877447/1‑71 (MQ=255) ttgtCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGc > 1:425298/1‑71 (MQ=255) gtCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGc > 1:659188/1‑69 (MQ=255) tGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCttt < 1:3040668/38‑1 (MQ=255) cccTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCa < 1:530133/71‑1 (MQ=255) cccTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCa < 1:4258947/71‑1 (MQ=255) cccTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCa < 1:4587056/71‑1 (MQ=255) cccTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCt < 1:1255481/44‑1 (MQ=255) cccTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCt < 1:1931378/44‑1 (MQ=255) cccTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGc < 1:4951309/70‑1 (MQ=255) ctctTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGacc < 1:1854818/52‑1 (MQ=255) tctTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGacc < 1:3778721/51‑1 (MQ=255) ctTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAAt > 1:4625491/1‑70 (MQ=255) tttGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAAt > 1:1486051/1‑69 (MQ=255) tttGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAAt < 1:4541205/69‑1 (MQ=255) tttGTTGAGATACAGCGGAATCCCGGCCTTTTTCa > 1:752775/1‑35 (MQ=255) tttGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAAt > 1:3815693/1‑69 (MQ=255) tttGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAAt > 1:2860377/1‑69 (MQ=255) tgttgAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGa < 1:4327752/69‑1 (MQ=255) aTACAGGGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaag > 1:658736/1‑70 (MQ=255) aTACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCa > 1:3225959/1‑52 (MQ=255) aTACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaag > 1:4580410/1‑70 (MQ=255) aTACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaag > 1:833171/1‑70 (MQ=255) aTACAGCGGAATCCCGGCCTATTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaag > 1:2855682/1‑70 (MQ=255) tACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATa < 1:3428480/66‑1 (MQ=255) tACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATa < 1:2399798/66‑1 (MQ=255) tACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATa < 1:2207924/66‑1 (MQ=255) aCATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaagca > 1:4148696/1‑70 (MQ=255) aCATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaagca > 1:3157848/1‑70 (MQ=255) aCAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaagca > 1:4006039/1‑70 (MQ=255) aGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGAt < 1:527245/62‑1 (MQ=255) aGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGAt < 1:3444084/62‑1 (MQ=255) | ATCATCATGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCA > W3110S.gb/4392026‑4392156 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |