| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,418,872 | A→G | 36.6% | *388* (TAA→TGA) | yjfC → | predicted synthetase/amidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,418,872 | 0 | A | G | 36.6% | 132.5 / 159.6 | 146 | *388* (TAA→TGA) | yjfC | predicted synthetase/amidase |
| Reads supporting (aligned to +/- strand): ref base A (41/51); new base G (36/17); total (77/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.31e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01 | |||||||||||
Only 100 of 150 total aligned reads displayed.
GATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATT > W3110S.gb/4418804‑4418940 | gATCCGTGAACATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAg < 1:1286165/71‑1 (MQ=255) aTCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATgaga < 1:4377134/71‑1 (MQ=255) ccGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAAt > 1:4856688/1‑71 (MQ=255) ccGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAAt > 1:3668015/1‑71 (MQ=255) ccGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAAt > 1:825829/1‑71 (MQ=255) ccGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAAt > 1:1586853/1‑71 (MQ=255) cGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATg > 1:3394875/1‑71 (MQ=255) gTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGt < 1:3397986/71‑1 (MQ=255) gAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGttt > 1:3336596/1‑71 (MQ=255) gAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGttt > 1:4382327/1‑71 (MQ=255) aaGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTCGATAAGAATGttt > 1:3249486/1‑70 (MQ=255) gATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAg > 1:3502489/1‑71 (MQ=255) aTAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAg > 1:789077/1‑70 (MQ=255) tAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCa < 1:4568727/71‑1 (MQ=255) aaCACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCaa > 1:1406595/1‑71 (MQ=255) cacacTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAAtc > 1:2489593/1‑71 (MQ=255) cacacTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAAtc > 1:3707294/1‑71 (MQ=255) cacacTGATCAACAAAGACACCTCACGTTACGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAAtc > 1:1131600/1‑71 (MQ=255) cTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAg < 1:973557/52‑1 (MQ=255) cTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCtt > 1:3796259/1‑71 (MQ=255) tGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCttt > 1:3894164/1‑71 (MQ=255) tGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCaa < 1:2923880/64‑1 (MQ=255) tCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTg > 1:2636401/1‑71 (MQ=255) cACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGt > 1:3038629/1‑71 (MQ=255) aCCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGt > 1:2767032/1‑51 (MQ=255) aCCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGt < 1:2698149/70‑1 (MQ=255) aCCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGt < 1:2598046/70‑1 (MQ=255) aCCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGt < 1:2494445/70‑1 (MQ=255) aCCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGt < 1:1900931/70‑1 (MQ=255) ccAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCa > 1:12809/1‑71 (MQ=255) aaGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGTCATGa < 1:4343191/71‑1 (MQ=255) aaGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATg > 1:1441641/1‑70 (MQ=255) gACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAAt > 1:4952594/1‑71 (MQ=255) acacCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATc > 1:3535483/1‑71 (MQ=255) ccTCACGTTTCGTTCCGCATTACATTGCTGGATgaga < 1:2096657/37‑1 (MQ=255) ccTCACGTTTCGTTCCGCATTACATTGCTGGATAAg < 1:4770301/36‑1 (MQ=255) ccTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCAt < 1:1328209/71‑1 (MQ=255) cTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTg < 1:4614947/58‑1 (MQ=255) tCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCa < 1:3081855/46‑1 (MQ=255) tCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATgg > 1:2470045/1‑71 (MQ=255) tCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCa < 1:4708520/46‑1 (MQ=255) tCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCa < 1:2932481/46‑1 (MQ=255) tCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCa < 1:1049061/46‑1 (MQ=255) tCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCa < 1:273134/46‑1 (MQ=255) tCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATg < 1:4161532/62‑1 (MQ=255) cACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATgg > 1:1703551/1‑70 (MQ=255) aCGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAAt > 1:980414/1‑46 (MQ=255) aCGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCttt > 1:4160695/1‑52 (MQ=255) aCGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCttt > 1:3513286/1‑52 (MQ=255) aCGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCttt > 1:1855840/1‑52 (MQ=255) aCGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATgg > 1:2158171/1‑69 (MQ=255) aCGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATgg > 1:4528610/1‑69 (MQ=255) aCGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGc < 1:1384451/70‑1 (MQ=255) aCGTTTCGATCCGCATTACATTGCTGGATAAGAATGTTTTAGCaa > 1:1472573/1‑45 (MQ=255) cGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAg > 1:2292261/1‑71 (MQ=255) gTTTCGTTCCGCATTACATTGCTGGATAAGAATGtttt < 1:2354759/38‑1 (MQ=255) gTTTCGTTCCGCATTACATTGCTGGATAAGAATGtttt < 1:2203895/38‑1 (MQ=255) gTTTCGTTCCGCATTACATTGCTGGATAAGAATGtttt < 1:564788/38‑1 (MQ=255) gTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTc > 1:1583278/1‑51 (MQ=255) gTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGt < 1:98419/71‑1 (MQ=255) tttCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTg < 1:3894735/71‑1 (MQ=255) tttCGTTCCGCATTACATTGCTGGATAAGAATGttt < 1:2723595/36‑1 (MQ=255) tttCGTTCCGCATTACATTGCTGGATAAGAATGttt < 1:399176/36‑1 (MQ=255) tttCGTTCCGCATTACATTGCTGGATAAGAATGttt < 1:2432984/36‑1 (MQ=255) tttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGc < 1:4290050/40‑1 (MQ=255) tttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGt > 1:1301372/1‑70 (MQ=255) tttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGt > 1:4709313/1‑70 (MQ=255) ttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGa > 1:1966893/1‑71 (MQ=255) ttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGa > 1:711000/1‑71 (MQ=255) ttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGa > 1:1953891/1‑71 (MQ=255) tCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGTCAt > 1:97637/1‑54 (MQ=255) tCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGAc > 1:928904/1‑71 (MQ=255) tCGTTCCGCATTACATTGCTGGATAAGAATGTTTTa > 1:2173697/1‑36 (MQ=255) cGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTg > 1:3447757/1‑49 (MQ=255) agTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGAc > 1:5040744/2‑70 (MQ=255) gTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCaa > 1:807282/1‑38 (MQ=255) gTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCaa > 1:5018590/1‑38 (MQ=255) gTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCa < 1:1205062/71‑1 (MQ=255) gTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCa < 1:3658766/71‑1 (MQ=255) ttCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATg < 1:3038661/52‑1 (MQ=255) ttCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCa < 1:2362686/70‑1 (MQ=255) ccGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATAc > 1:1879012/1‑71 (MQ=255) cGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACt < 1:465950/71‑1 (MQ=255) cGCATTACATTGCTGGATAAGAATGTTTTAGCAATctct < 1:40597/39‑1 (MQ=255) gCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTa < 1:904902/71‑1 (MQ=255) gCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTa < 1:5038637/71‑1 (MQ=255) aTTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCAt > 1:14791/1‑45 (MQ=255) ttACATTGCTGGATGAGAATGTTTTAGCAATctct < 1:3870262/35‑1 (MQ=255) aCATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGa > 1:2132761/1‑58 (MQ=255) aCATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTaa > 1:2829201/1‑67 (MQ=255) aCATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGt > 1:278997/1‑71 (MQ=255) cATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGt > 1:1392667/1‑70 (MQ=255) cATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTg < 1:3065303/71‑1 (MQ=255) ttGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGAc < 1:2269550/71‑1 (MQ=255) ttGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGAc < 1:411400/71‑1 (MQ=255) gCTGGATAAGAATGTTTTAGCAATCTCTTTCTTTCATGAATCCATGGCAGTGACCATACTAATGGTg < 1:2239736/67‑1 (MQ=255) cTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGc < 1:3895760/71‑1 (MQ=255) tGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTg < 1:1037045/65‑1 (MQ=255) gATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCAt > 1:4018327/1‑71 (MQ=255) tAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCAtt > 1:5008583/1‑70 (MQ=255) | GATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATT > W3110S.gb/4418804‑4418940 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |