| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,443,410 | G→A | 31.6% | L179L (CTG→TTG) | ytfJ ← | predicted transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,443,410 | 0 | G | A | 31.6% | 81.2 / 45.0 | 57 | L179L (CTG→TTG) | ytfJ | predicted transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (16/23); new base A (14/4); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.19e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGACCA > W3110S.gb/4443341‑4443477 | tAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTgcag > 1:4582448/1‑70 (MQ=255)tAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTgcag > 1:2602940/1‑70 (MQ=255) gATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGcaacag > 1:3863894/1‑71 (MQ=255) gATAACGATAAGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGcagcag > 1:4253407/1‑71 (MQ=255) gATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCAt < 1:1189093/71‑1 (MQ=255) gATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCAt < 1:3418634/71‑1 (MQ=255) gATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCAt > 1:3678353/1‑71 (MQ=255) aTAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATc < 1:3440673/71‑1 (MQ=255) aTAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCAt > 1:4208238/1‑70 (MQ=255) aTAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATc < 1:5025293/71‑1 (MQ=255) gAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTtgc < 1:1344924/71‑1 (MQ=255) aaaTTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCACTtgctg < 1:3029105/71‑1 (MQ=255) aaTTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTtgctg > 1:3366899/1‑70 (MQ=255) ttattTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACt < 1:125499/63‑1 (MQ=255) ttattTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCAc > 1:4003263/1‑62 (MQ=255) ttattTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCACt > 1:4657667/1‑63 (MQ=255) ttattTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCACTTGCTGCAc < 1:5125062/71‑1 (MQ=255) ttattTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGAAGCAGGTCCATCACt < 1:1745639/63‑1 (MQ=255) attTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACt < 1:2031007/61‑1 (MQ=255) attTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACt < 1:4388598/61‑1 (MQ=255) cAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTgcag < 1:2122106/41‑1 (MQ=255) aGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGgt < 1:1700697/71‑1 (MQ=255) aaTTTATGTTATTGCCATTATTTGAGCAACTTTTgcagc < 1:2892934/39‑1 (MQ=255) aaTTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGgtgt < 1:2845017/71‑1 (MQ=255) aaTTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGgtg > 1:5118150/1‑70 (MQ=255) ttATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAg > 1:1363280/1‑71 (MQ=255) ttATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAg > 1:4119916/1‑71 (MQ=255) tATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCAATtgc > 1:4416050/1‑50 (MQ=255) ttATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAg > 1:2418824/1‑66 (MQ=255) tGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAgcg > 1:126621/1‑64 (MQ=255) ccATTATTTGAGCAACTTTTGCAACAGGTCCATCa > 1:4175008/1‑35 (MQ=255) ccATTATTTGAGCAACTTTTGCAACAGGTCCATCAc > 1:1403497/1‑36 (MQ=255) ccATTATTTGAGCAACTTTTGCAACAGGTCCATCAc > 1:1371383/1‑36 (MQ=255) ccATTATTTGAGCAACTTTTGCAACAGGTCCATCAc > 1:3231219/1‑36 (MQ=255) cATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCttt < 1:3293584/71‑1 (MQ=255) attattTGAGCAACTTTTGCAGCAGGTCCATCACTtgctg < 1:3573717/40‑1 (MQ=255) attattTGAGCAACTTTTGCAGCAGGTCCATCACTtgctg < 1:620321/40‑1 (MQ=255) ttattTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACctct < 1:59518/46‑1 (MQ=255) ttattTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACctct < 1:408346/46‑1 (MQ=255) tattTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCa > 1:2768341/1‑40 (MQ=255) tattTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCa > 1:2605365/1‑40 (MQ=255) attTGAGCAACTTTTGCAGCAGGTCCATCACTtgct > 1:796637/1‑36 (MQ=255) attTGAGCAACTTTTGCAGCAGGTCCATCACTtgct > 1:2842641/1‑36 (MQ=255) attTGAGCAAATTTTGCAGCAGGTCCATCACTtgctgc < 1:4447274/38‑1 (MQ=255) tttGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGccc < 1:3063958/71‑1 (MQ=255) tttGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGccc < 1:3895497/71‑1 (MQ=255) tttGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGccc < 1:2809598/71‑1 (MQ=255) tttGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGcc < 1:3269259/70‑1 (MQ=255) ttGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCAc > 1:4668892/1‑38 (MQ=255) cAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCa < 1:687215/71‑1 (MQ=255) aaCTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCAc < 1:2637776/71‑1 (MQ=255) aCTTTTGCAACAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCAcg > 1:4915825/1‑71 (MQ=255) cTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGgt < 1:723034/40‑1 (MQ=255) tttGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGgtgt > 1:1952984/1‑40 (MQ=255) tttGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGgtgt > 1:649190/1‑40 (MQ=255) tttGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGa > 1:4821292/1‑71 (MQ=255) tttGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGa > 1:5119964/1‑71 (MQ=255) tttGCAACAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCtt < 1:5089025/54‑1 (MQ=255) tttGCAACAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGa > 1:3063095/1‑71 (MQ=255) tttGCAACAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCACGTCTTTGGCCCATTGCa > 1:1412369/1‑66 (MQ=255) tGCAGCGGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGAc > 1:658750/1‑70 (MQ=255) tGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGAc > 1:1643923/1‑70 (MQ=255) tGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGAc > 1:1220122/1‑70 (MQ=255) cagcagGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGACCa < 1:779065/70‑1 (MQ=255) | TAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGACCA > W3110S.gb/4443341‑4443477 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |