| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,477,260 | G→A | 42.6% | intergenic (‑45/+236) | pyrL ← / ← yjgH | pyrBI operon leader peptide/predicted mRNA endoribonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,477,260 | 0 | G | A | 42.6% | 38.8 / 83.2 | 68 | intergenic (‑45/+236) | pyrL/yjgH | pyrBI operon leader peptide/predicted mRNA endoribonuclease |
| Reads supporting (aligned to +/- strand): ref base G (19/20); new base A (17/12); total (36/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATG > W3110S.gb/4477190‑4477330 | aCAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAg < 1:1366111/71‑1 (MQ=255) aaaaTGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgcg > 1:4845669/1‑71 (MQ=255) aaaaTGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgcg > 1:4409327/1‑71 (MQ=255) gAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGc < 1:254923/71‑1 (MQ=255) gAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGc < 1:2827333/71‑1 (MQ=255) acacacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAt > 1:3476116/1‑71 (MQ=255) cacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTg < 1:3504881/71‑1 (MQ=255) acTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGa > 1:3351540/1‑71 (MQ=255) cTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGa > 1:3323878/1‑70 (MQ=255) ccATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCaa > 1:1275365/1‑70 (MQ=255) tACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgcg < 1:2874452/48‑1 (MQ=255) tACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAg < 1:2407021/55‑1 (MQ=255) tACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATAtt > 1:1688959/1‑71 (MQ=255) cATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGa > 1:1774089/1‑60 (MQ=255) cATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATAtttt > 1:3829387/1‑71 (MQ=255) tcctccCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAg < 1:2206481/51‑1 (MQ=255) ctccCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt < 1:1611998/54‑1 (MQ=255) ctccCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGt > 1:4820524/1‑69 (MQ=255) ctccCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGt > 1:1124691/1‑69 (MQ=255) ctccCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGt > 1:4581817/1‑69 (MQ=255) atccCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGt > 1:2047440/2‑69 (MQ=255) cccGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAg > 1:3537143/1‑70 (MQ=255) cccGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGAGGCGCAAGCATTTGATGCAATATTTTGTCAgc > 1:2543330/1‑71 (MQ=255) ccGGCAAATTGTCCGGCATTATACTCATCGTCAGAAg > 1:1097808/1‑37 (MQ=255) ccGGCAAATTGTCCGGCATTATACTCATCGTCAGAAg > 1:14851/1‑37 (MQ=255) gCAAATTGTCCTGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt < 1:325986/48‑1 (MQ=255) gCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCaa < 1:1243334/71‑1 (MQ=255) aaTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACg > 1:2225907/1‑71 (MQ=255) aaTTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACg < 1:3033652/71‑1 (MQ=255) ttGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAttt > 1:71022/1‑43 (MQ=255) ttGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATg > 1:49515/1‑47 (MQ=255) ttGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCaa > 1:2269044/1‑50 (MQ=255) ttGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCaa > 1:4246708/1‑50 (MQ=255) ttGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCa > 1:3492416/1‑60 (MQ=255) tGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt < 1:2281010/42‑1 (MQ=255) tGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGtt > 1:4134959/1‑71 (MQ=255) gTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGa > 1:3628806/1‑43 (MQ=255) gTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGa > 1:4655253/1‑43 (MQ=255) gTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGa > 1:2276553/1‑43 (MQ=255) gTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATATGa > 1:2051336/1‑43 (MQ=255) tCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTa < 1:395499/71‑1 (MQ=255) ccGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGt < 1:1810595/67‑1 (MQ=255) ccGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTAt > 1:713281/1‑71 (MQ=255) ccGGCATCATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGt < 1:165034/67‑1 (MQ=255) gCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTg < 1:2957751/71‑1 (MQ=255) gCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTa > 1:1244383/1‑67 (MQ=255) cATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGa < 1:440857/71‑1 (MQ=255) cATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGGTTATTTGa < 1:4975935/71‑1 (MQ=255) tataCTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAAt > 1:1402540/1‑70 (MQ=255) ataCTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAAtt > 1:2448843/1‑70 (MQ=255) ataCTCATCGTCAGAAACGGCGCAAGCATTTGATGCAat > 1:4068185/1‑39 (MQ=255) ataCTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGt < 1:4478388/46‑1 (MQ=255) ataCTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGt < 1:2176178/46‑1 (MQ=255) ataCTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAAtt < 1:1773506/70‑1 (MQ=255) cTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAata < 1:1468244/37‑1 (MQ=255) cTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGTAAACGGTTTATTTGAATTaaa < 1:4234892/70‑1 (MQ=255) cTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTaaa < 1:1812069/70‑1 (MQ=255) cTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTaaa < 1:24202/70‑1 (MQ=255) cATCGTCAGAAGCGGCGCAAGCATTTGATGCAATAtt > 1:4925299/1‑37 (MQ=255) cATCGTCAGAAGCGGCGCAAGCATTTGATGCAATAtt > 1:5121437/1‑37 (MQ=255) aTCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTg > 1:2082171/1‑60 (MQ=255) aTCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGt > 1:2074439/1‑70 (MQ=255) tCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTc < 1:4017413/40‑1 (MQ=255) tCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTc < 1:2484271/40‑1 (MQ=255) tCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTAAGCGCAAACGGTTTATTTg < 1:2219651/59‑1 (MQ=255) tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCa < 1:1024536/41‑1 (MQ=255) tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCa < 1:178944/71‑1 (MQ=255) gTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTaa < 1:2569229/63‑1 (MQ=255) gTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAg > 1:315002/1‑71 (MQ=255) cAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAgg > 1:3463994/1‑70 (MQ=255) aGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGta > 1:859326/1‑71 (MQ=255) aGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGt < 1:500462/70‑1 (MQ=255) aGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGt < 1:4033791/70‑1 (MQ=255) aGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTatat < 1:4583771/71‑1 (MQ=255) gcggcgCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATg > 1:1348462/1‑71 (MQ=255) gcggcgCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATg > 1:4320019/1‑71 (MQ=255) gcggcgCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATg > 1:3459505/1‑71 (MQ=255) | ACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATG > W3110S.gb/4477190‑4477330 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |