| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,494,403 | G→A | 42.6% | L115L (CTG→TTG) | yjgR ← | predicted ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,494,403 | 0 | G | A | 42.6% | 21.2 / 56.9 | 47 | L115L (CTG→TTG) | yjgR | predicted ATPase |
| Reads supporting (aligned to +/- strand): ref base G (15/12); new base A (8/12); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01 | |||||||||||
ATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGATTGGCA > W3110S.gb/4494339‑4494473 | atatTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAAATCCg < 1:380451/70‑1 (MQ=255)atatTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAAATCCg < 1:3620452/70‑1 (MQ=255) tCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAAATCCGAAAc < 1:4446016/70‑1 (MQ=255) cAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCg > 1:4000559/1‑71 (MQ=255) aGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGt > 1:3669465/1‑71 (MQ=255) cACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTc > 1:736585/1‑70 (MQ=255) cACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCg > 1:3195794/1‑71 (MQ=255) aCGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAAATCCGAAACCGTCGc < 1:3511209/71‑1 (MQ=255) gCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAAATCCGa > 1:5129760/1‑60 (MQ=255) gCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAAATCCGa > 1:481690/1‑60 (MQ=255) cAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCg > 1:2070293/1‑70 (MQ=255) aTTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAg > 1:3166038/1‑49 (MQ=255) ttGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAAATCCGAAACCGTCGCCCGCACCg < 1:4744095/71‑1 (MQ=255) aTCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGc < 1:1029902/71‑1 (MQ=255) aTCGTTGAGATTCAACAGTAGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGc < 1:3821571/71‑1 (MQ=255) gTTGAGATTCAACAGTCGTG‑CAGCAACAGCGGCCCCAAATCCGAAACCGTCGCCCGCACCGGATGGCCttt < 1:1694043/71‑1 (MQ=255) ttGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAAATCCGAAACCGTCGCCCGCACCg < 1:1190188/61‑1 (MQ=255) tGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGa > 1:3023886/1‑62 (MQ=255) gagaTTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTtc < 1:4643915/70‑1 (MQ=255) agaTTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAAc > 1:4756593/1‑44 (MQ=255) agaTTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAAc > 1:4401117/1‑44 (MQ=255) aTTCAACAGTCGTGCCAGCAACAGCGGCCCCAAATCCGAAACCGTCGCCCGCACCGGATGGCCt < 1:2246007/64‑1 (MQ=255) cAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCa < 1:1941851/70‑1 (MQ=255) aaCAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCaaa < 1:1185980/71‑1 (MQ=255) aCAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAg < 1:4591706/71‑1 (MQ=255) tCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGc < 1:4475361/39‑1 (MQ=255) tCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCa > 1:586089/1‑44 (MQ=255) tCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGatat < 1:1006398/71‑1 (MQ=255) tCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGatat > 1:4816913/1‑71 (MQ=255) tCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGatat > 1:1179730/1‑71 (MQ=255) tCGTGCCAGCAACAGCGGCCCCAAATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGata > 1:2409994/1‑70 (MQ=255) gTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGccc > 1:4296609/1‑39 (MQ=255) gTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGAt > 1:1316085/1‑48 (MQ=255) aGCAACAGCGGCCCCAAATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCacac > 1:5067202/1‑71 (MQ=255) gCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCAcacc < 1:1030031/71‑1 (MQ=255) gCAACAGCGGCCCCAAATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGa < 1:3479258/60‑1 (MQ=255) cAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAg < 1:3213516/58‑1 (MQ=255) cAACAGCGGCCCCAAATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATcc > 1:1165759/1‑64 (MQ=255) aaCAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGa > 1:2396783/1‑39 (MQ=255) aaCAGCGGCCCCAAATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGata > 1:77903/1‑60 (MQ=255) aaCAGCGGCCCCAAATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGata > 1:1407572/1‑60 (MQ=255) aCAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGcc < 1:2591561/52‑1 (MQ=255) aCAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCAcaccac > 1:2291936/1‑70 (MQ=255) cGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCg < 1:4838539/71‑1 (MQ=255) ggCCCCAAATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATccc > 1:4464597/1‑58 (MQ=255) ccAAATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCca < 1:8320/55‑1 (MQ=255) ccAAATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCg < 1:713564/66‑1 (MQ=255) ccAAATCCGAAACCGTCGCCCGCACCGGATGACATTTCTCGCCAAAGATATCCCACACCACCACCg < 1:625665/66‑1 (MQ=255) gATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCca > 1:4406663/1‑52 (MQ=255) gATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGATTGGCa > 1:4883319/1‑71 (MQ=255) | ATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGATTGGCA > W3110S.gb/4494339‑4494473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |