| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,496,317 | G→A | 44.4% | G297G (GGC→GGT) | idnT ← | L‑idonate and D‑gluconate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,496,317 | 0 | G | A | 44.4% | 23.9 / 80.2 | 63 | G297G (GGC→GGT) | idnT | L‑idonate and D‑gluconate transporter |
| Reads supporting (aligned to +/- strand): ref base G (17/18); new base A (16/12); total (33/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01 | |||||||||||
CACCGCTATCTACTAATACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAGTG > W3110S.gb/4496247‑4496381 | cacCGCTATCTACTAATACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAgcg > 1:1416284/1‑71 (MQ=255) tCTACTAATACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAg < 1:1259373/70‑1 (MQ=255) tCTACTAATACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCACCtata < 1:1584472/69‑1 (MQ=255) ctactaATACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGaa > 1:3172690/1‑71 (MQ=255) actaATACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGaa > 1:1688009/1‑69 (MQ=255) ctaATACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGa < 1:4124198/67‑1 (MQ=255) ctaATACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATcc > 1:806745/1‑71 (MQ=255) aaTACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATccc > 1:2526567/1‑70 (MQ=255) aTACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCTATAGCGCCTATAGAATcccc < 1:4472233/70‑1 (MQ=255) aTACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATcccc > 1:467950/1‑70 (MQ=255) aTACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCACCTATAGAATcccc < 1:531045/70‑1 (MQ=255) aCCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAAt > 1:3071412/1‑71 (MQ=255) ccTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAAt > 1:3837215/1‑70 (MQ=255) ccTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCACCTATAGAATCCCCAATg < 1:1341204/71‑1 (MQ=255) aaaCGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCtata < 1:3588224/52‑1 (MQ=255) aaaCGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCa > 1:3415400/1‑71 (MQ=255) aaaCGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCACCTATAg > 1:2420011/1‑53 (MQ=255) aaaCGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCACCTATAg > 1:3155355/1‑53 (MQ=255) aaCGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCAt > 1:761045/1‑71 (MQ=255) aaCGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCAt > 1:2821660/1‑71 (MQ=255) aCGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATg < 1:4326505/71‑1 (MQ=255) cgcgCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGa < 1:3907178/71‑1 (MQ=255) gcgcCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGa < 1:3901374/70‑1 (MQ=255) ccgccgccAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGAttt < 1:1082472/70‑1 (MQ=255) ccgccgccAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGAttt < 1:2692868/70‑1 (MQ=255) ccgccgccAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTg < 1:4053977/71‑1 (MQ=255) gccgccAGCGATAATAAACACAATCATCGCGATAGCGCCTATAg > 1:1190750/1‑44 (MQ=255) gccAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGa < 1:408379/71‑1 (MQ=255) cGATAATAAACTCAATCATCGCGATAGCGCCTATAGaa < 1:2702192/38‑1 (MQ=255) gATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGa > 1:4710849/1‑56 (MQ=255) gATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGa > 1:762107/1‑56 (MQ=255) gATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGa > 1:201025/1‑56 (MQ=255) gATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGAtt < 1:410545/58‑1 (MQ=255) gATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGAtt < 1:501581/58‑1 (MQ=255) taataaACACAATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCATGAtt < 1:4427310/56‑1 (MQ=255) taataaACACAATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCATGAtt < 1:276567/56‑1 (MQ=255) aataaACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGAt > 1:4779529/1‑63 (MQ=255) ataaACACAATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCATGATTTg > 1:1177966/1‑56 (MQ=255) aaaCACAATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGAt > 1:3883414/1‑60 (MQ=255) aaaCACAATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGAt > 1:1801532/1‑60 (MQ=255) aaaCACAATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGAt > 1:1109896/1‑60 (MQ=255) acacAATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTa < 1:4777175/71‑1 (MQ=255) acacAATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTa < 1:2249191/71‑1 (MQ=255) acacAATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTa < 1:2396069/71‑1 (MQ=255) cacaATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTAc > 1:765213/1‑71 (MQ=255) cacaATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTAc > 1:1075894/1‑71 (MQ=255) cacaATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCAt > 1:560762/1‑45 (MQ=255) cacaATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCAt > 1:252267/1‑45 (MQ=255) caATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGt < 1:2067052/71‑1 (MQ=255) tcatcGCGATAGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGAt < 1:4760783/52‑1 (MQ=255) catcGCGATAGCACCTATAGAATCCCCAATGATATCCATGATTTGc > 1:4867529/1‑46 (MQ=255) catcGCGATAGCACCTATAGAATCCCCAATGATATCCATGATTTGc > 1:3196473/1‑46 (MQ=255) catcGCGATAGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCTAt < 1:3799585/51‑1 (MQ=255) atcGCGATAGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCg > 1:316805/1‑68 (MQ=255) atcGCGATAGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCg > 1:5062159/1‑68 (MQ=255) tcGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTc < 1:1775721/46‑1 (MQ=255) tcGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTcc < 1:4347108/58‑1 (MQ=255) tcGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTcc < 1:1707825/58‑1 (MQ=255) gcgATAGCACCTATAGAACCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCa < 1:393744/70‑1 (MQ=255) cgATAGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGAt < 1:3927853/46‑1 (MQ=255) gATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAgtg > 1:4789392/1‑71 (MQ=255) gATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAgt > 1:1568155/1‑70 (MQ=255) aGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGATgg > 1:3612968/1‑44 (MQ=255) aGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGATgg > 1:4901461/1‑44 (MQ=255) | CACCGCTATCTACTAATACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAGTG > W3110S.gb/4496247‑4496381 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |