| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,561,090 | G→A | 37.6% | intergenic (‑88/‑585) | yjiC ← / → yjiD | hypothetical protein/DNA replication/recombination/repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,561,090 | 0 | G | A | 37.6% | 81.2 / 91.0 | 85 | intergenic (‑88/‑585) | yjiC/yjiD | hypothetical protein/DNA replication/recombination/repair protein |
| Reads supporting (aligned to +/- strand): ref base G (30/23); new base A (14/18); total (44/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTAAAGT > W3110S.gb/4561021‑4561156 | gTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAcatg > 1:2742584/1‑70 (MQ=255) tttCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAcatgcatg > 1:1147567/1‑71 (MQ=255) tttCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAcatgcatg > 1:137671/1‑71 (MQ=255) tttCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAcatgcat < 1:1896731/70‑1 (MQ=255) tCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGtt > 1:4917854/1‑71 (MQ=255) tCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGt < 1:162863/70‑1 (MQ=255) tCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGt < 1:2316033/70‑1 (MQ=255) cACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGttt > 1:4290369/1‑71 (MQ=255) cACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGt < 1:3156636/69‑1 (MQ=255) cACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGtt < 1:4625592/70‑1 (MQ=255) aCTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGttt > 1:4529945/1‑70 (MQ=255) aCTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGttt < 1:3271451/70‑1 (MQ=255) aCTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTc < 1:325048/71‑1 (MQ=255) aCTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTc < 1:1342745/71‑1 (MQ=255) aCTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTc < 1:4648137/71‑1 (MQ=255) gATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCtgt > 1:2789673/1‑71 (MQ=255) aTGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCtgtt > 1:515597/1‑71 (MQ=255) ttAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCtgttgt < 1:4071754/69‑1 (MQ=255) tAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGt > 1:2328146/1‑71 (MQ=255) aGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGt < 1:276547/70‑1 (MQ=255) aGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGt < 1:3725502/70‑1 (MQ=255) aaaTAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTa > 1:2092431/1‑64 (MQ=255) aaaTAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGatat > 1:2360062/1‑71 (MQ=255) aaTAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGatat > 1:3475299/1‑70 (MQ=255) aaTAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATc < 1:3391707/71‑1 (MQ=255) aaTAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGt > 1:4713090/1‑53 (MQ=255) gATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAAct < 1:3653881/71‑1 (MQ=255) aaaTTTTTCTGTAGTAAGAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTa > 1:4201940/1‑71 (MQ=255) aaTTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTa < 1:4689454/70‑1 (MQ=255) aaTTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTa < 1:2945950/70‑1 (MQ=255) aaTTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTAATATCAACTCTa < 1:1556962/70‑1 (MQ=255) ttttCTGTAGTAAAAAGAGAAGTAAACAAATGAcatg > 1:615551/1‑37 (MQ=255) ttttCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACg > 1:224535/1‑71 (MQ=255) ttttCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACg > 1:1627198/1‑71 (MQ=255) tagtaAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGatat > 1:3069120/1‑71 (MQ=255) tagtaAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGatat > 1:2321469/1‑71 (MQ=255) tagtaAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGatat > 1:2849912/1‑71 (MQ=255) tagtaAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGa > 1:4190646/1‑49 (MQ=255) tagtaAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGa > 1:1346202/1‑49 (MQ=255) tagtaAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGa > 1:2003833/1‑49 (MQ=255) tagtaAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGa > 1:3405774/1‑49 (MQ=255) gtaAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATAtt < 1:449119/70‑1 (MQ=255) taAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATAtt < 1:1796161/69‑1 (MQ=255) taAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTa > 1:295141/1‑70 (MQ=255) taAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAActc < 1:627711/55‑1 (MQ=255) taAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAActc < 1:1500080/55‑1 (MQ=255) taAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTa < 1:2241099/57‑1 (MQ=255) aaaaaGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGa > 1:518350/1‑45 (MQ=255) aaaaGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGa > 1:547239/1‑44 (MQ=255) aaaaGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTaaa > 1:3269290/1‑70 (MQ=255) aaaaGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAg > 1:105726/1‑71 (MQ=255) aaaaGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAg > 1:3225770/1‑71 (MQ=255) aaaaGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAg > 1:3655929/1‑71 (MQ=255) aaGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATAtt < 1:1754987/65‑1 (MQ=255) aaGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAggg < 1:4309045/71‑1 (MQ=255) aaGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGatat > 1:4348357/1‑45 (MQ=255) agagAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAggg > 1:3245786/1‑70 (MQ=255) aaGTAAACAAATGACATACATGTATCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGa < 1:2692849/71‑1 (MQ=255) gTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCaa > 1:939018/1‑41 (MQ=255) gTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCaa > 1:191356/1‑41 (MQ=255) gTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCaa > 1:5027803/1‑41 (MQ=255) gTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTaca > 1:3843604/1‑48 (MQ=255) gTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTaca > 1:3269629/1‑48 (MQ=255) gTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGaaa > 1:541378/1‑71 (MQ=255) gTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGaaa > 1:942880/1‑71 (MQ=255) gTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGaaa < 1:601684/71‑1 (MQ=255) tAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGa < 1:1862005/68‑1 (MQ=255) tAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGa < 1:3703547/68‑1 (MQ=255) tAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGa < 1:3348829/68‑1 (MQ=255) tAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGa < 1:2579726/68‑1 (MQ=255) aaCAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTaa > 1:1186095/1‑57 (MQ=255) aaCAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAacac > 1:377458/1‑71 (MQ=255) aaCAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAaca < 1:3983141/70‑1 (MQ=255) aaCAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAaca < 1:740361/70‑1 (MQ=255) aaCAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGaaa > 1:2021741/1‑68 (MQ=255) aCAAATGACATGCATGTTTCTGTTGTAGTGATATCaa < 1:4384952/37‑1 (MQ=255) aCAAATGACATGCATGTTTCTGTTGTAGTGATATCaa < 1:4390962/37‑1 (MQ=255) aaaTGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGa < 1:401429/63‑1 (MQ=255) aaTGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTa < 1:3963664/71‑1 (MQ=255) aaTGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTa < 1:853326/71‑1 (MQ=255) aaTGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTa < 1:1957897/71‑1 (MQ=255) aTGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTaa < 1:3039875/71‑1 (MQ=255) aTGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTaa < 1:3184865/71‑1 (MQ=255) tGACATGCATGTTTCTGTTGTAGTGATATCAActc < 1:288127/35‑1 (MQ=255) tGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACActct > 1:1410910/1‑68 (MQ=255) gACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTAAAg > 1:2779833/1‑71 (MQ=255) aCATGCATGTTTCTGTTGTAGTGATATCAACTCTa > 1:3245891/1‑35 (MQ=255) aCATGCATGTTTCTGTTGTAGTGATATCAACTCTa > 1:4474029/1‑35 (MQ=255) aCATGCATGTTTCTGTTGTAGTGATATCAACTCTa > 1:872071/1‑35 (MQ=255) aCATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTAAAGt < 1:1643356/71‑1 (MQ=255) gcatgTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGa < 1:220538/54‑1 (MQ=255) | GTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTAAAGT > W3110S.gb/4561021‑4561156 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |