Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,563,589 T→A 100% K207M (AAG→ATG)  iadA ← isoaspartyl dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,563,5890TA100.0% 226.8 / NA 75K207M (AAG→ATG) iadAisoaspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (40/35);  total (40/35)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGA  >  W3110S.gb/4563526‑4563656
                                                               |                                                                   
ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc                                                               >  1:1921905/1‑70 (MQ=255)
      agcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc                                                        >  1:3304132/1‑71 (MQ=255)
        cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                                      <  1:4258307/71‑1 (MQ=255)
          tGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa                                                                >  1:4188936/1‑59 (MQ=255)
           gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa                                                                <  1:403860/58‑1 (MQ=255)
           gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgt                                                   >  1:601822/1‑71 (MQ=255)
           gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg                                                    >  1:3130229/1‑70 (MQ=255)
            cTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg                                                  <  1:2088378/71‑1 (MQ=255)
            cTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg                                                  <  1:3891121/71‑1 (MQ=255)
            cTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg                                                  <  1:4637880/71‑1 (MQ=255)
               aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg                                                 <  1:4697636/69‑1 (MQ=255)
               aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa                                               >  1:1844175/1‑71 (MQ=255)
                tCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAac                                              <  1:2196707/71‑1 (MQ=255)
                tCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAac                                              <  1:2174120/71‑1 (MQ=255)
                 cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc                                                               >  1:4718778/1‑53 (MQ=255)
                 cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt                                                     >  1:644829/1‑63 (MQ=255)
                 cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                             >  1:4132303/1‑71 (MQ=255)
                 cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                             >  1:4187979/1‑71 (MQ=255)
                 cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                             >  1:13892/1‑71 (MQ=255)
                  ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                                      <  1:440645/61‑1 (MQ=255)
                  ggCACATCGCAGTTTTCCAGCAGGGCATAAATAGGCTGTAACGCCATTTTACt                                                              <  1:3241060/53‑1 (MQ=255)
                   gCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACg                                           <  1:1606902/71‑1 (MQ=255)
                     acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                         <  1:4686244/71‑1 (MQ=255)
                       aTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt                                                              <  1:4270545/48‑1 (MQ=255)
                       aTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt                                                              <  1:4264784/48‑1 (MQ=255)
                          tcAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc                                                               >  1:146231/2‑44 (MQ=255)
                          gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt                                                                 >  1:1539310/1‑42 (MQ=255)
                          gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc                                                               >  1:2339214/1‑44 (MQ=255)
                          gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc                                                               >  1:2031202/1‑44 (MQ=255)
                          gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc                                                               >  1:4331642/1‑44 (MQ=255)
                          gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                             >  1:4710180/1‑62 (MQ=255)
                          gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                             >  1:5032353/1‑62 (MQ=255)
                           cAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc                                   <  1:5069817/71‑1 (MQ=255)
                            aGTTTTCCAGCAGGTCATATATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg                                                  <  1:1726036/55‑1 (MQ=255)
                             gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt                                                              <  1:4219566/42‑1 (MQ=255)
                             gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt                                                              <  1:4940639/42‑1 (MQ=255)
                              ttttCCAGCAGGTCATAAATAGGCTGTAACGCCatttt                                                                 <  1:2718534/38‑1 (MQ=255)
                              ttttCCAGCAGGTCATAAATAGGCTGTAACGCCatttt                                                                 <  1:5045399/38‑1 (MQ=255)
                              ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac                                            <  1:3974134/59‑1 (MQ=255)
                              ttttCCAGCAGGTCATAAATAGGCTGTAACACCATTTTACTGTCGCCCATGTGGAacac                                            <  1:3107805/59‑1 (MQ=255)
                                ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc                                                        >  1:362692/1‑45 (MQ=255)
                                ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt                              >  1:1442658/1‑71 (MQ=255)
                                  ccAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGttt                             <  1:333680/70‑1 (MQ=255)
                                  ccAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGttt                             <  1:5048555/70‑1 (MQ=255)
                                   cagcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc                           >  1:630112/1‑71 (MQ=255)
                                       agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa                                               >  1:738867/1‑47 (MQ=255)
                                       agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa                                               >  1:1167588/1‑47 (MQ=255)
                                         gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                         <  1:4323377/51‑1 (MQ=255)
                                         gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                         <  1:2003569/51‑1 (MQ=255)
                                           cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGa                                                >  1:926099/1‑42 (MQ=255)
                                           cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg                    >  1:1550544/1‑70 (MQ=255)
                                           cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg                    >  1:5010585/1‑70 (MQ=255)
                                           cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg                    >  1:2042610/1‑70 (MQ=255)
                                            atatatAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGc                   >  1:2124657/1‑70 (MQ=255)
                                            aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGc                   <  1:1092548/70‑1 (MQ=255)
                                             tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc                       <  1:1331499/65‑1 (MQ=255)
                                              aaaTAGGCTGTAACGCCATTTTACTGTCGCCCAtg                                                    <  1:4670801/35‑1 (MQ=255)
                                              aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa                >  1:684631/1‑71 (MQ=255)
                                               aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa                >  1:1242590/1‑70 (MQ=255)
                                               aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa                <  1:1472584/70‑1 (MQ=255)
                                               aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa                <  1:2716664/70‑1 (MQ=255)
                                                 tAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCg             >  1:1899219/1‑71 (MQ=255)
                                                      tGTAACGCCATTTTACTGTCGCCCATGTGGAACACg                                           <  1:4111755/36‑1 (MQ=255)
                                                      tGTAACGCCATTTTACTGTCGCCCATGTGGAACACg                                           <  1:3178710/36‑1 (MQ=255)
                                                      tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAc        >  1:1792482/1‑71 (MQ=255)
                                                        tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa                >  1:3267798/1‑61 (MQ=255)
                                                        tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa                >  1:886192/1‑61 (MQ=255)
                                                        tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcg       <  1:3698123/70‑1 (MQ=255)
                                                        tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGACAGGTTTACCGCCGAGCaaa                >  1:4897163/1‑61 (MQ=255)
                                                         aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGa                                       >  1:2115587/1‑37 (MQ=255)
                                                         aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGa                                       >  1:3333294/1‑37 (MQ=255)
                                                         aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg     >  1:4988118/1‑71 (MQ=255)
                                                         aaCGCCATTATACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg     >  1:1778643/1‑71 (MQ=255)
                                                          aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGAc                                      >  1:959862/1‑37 (MQ=255)
                                                            gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGa                     >  1:2369320/1‑52 (MQ=255)
                                                            gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGa  <  1:4505697/71‑1 (MQ=255)
                                                            gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGa  <  1:3456757/71‑1 (MQ=255)
                                                            gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGa  <  1:3544326/71‑1 (MQ=255)
                                                               |                                                                   
GGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGA  >  W3110S.gb/4563526‑4563656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: