Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	4,563,589	T→A	100%	K207M (AAG→ATG)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,563,589	0	T	A	100.0%	226.8 / NA	75	K207M (AAG→ATG)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (40/35);  total (40/35)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGA  >  W3110S.gb/4563526‑4563656                                                                |                                                                    ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc                                                               >  1:1921905/1‑70 (MQ=255)       agcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc                                                        >  1:3304132/1‑71 (MQ=255)         cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                                      <  1:4258307/71‑1 (MQ=255)           tGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa                                                                >  1:4188936/1‑59 (MQ=255)            gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa                                                                <  1:403860/58‑1 (MQ=255)            gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgt                                                   >  1:601822/1‑71 (MQ=255)            gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg                                                    >  1:3130229/1‑70 (MQ=255)             cTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg                                                  <  1:2088378/71‑1 (MQ=255)             cTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg                                                  <  1:3891121/71‑1 (MQ=255)             cTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg                                                  <  1:4637880/71‑1 (MQ=255)                aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg                                                 <  1:4697636/69‑1 (MQ=255)                aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa                                               >  1:1844175/1‑71 (MQ=255)                 tCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAac                                              <  1:2196707/71‑1 (MQ=255)                 tCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAac                                              <  1:2174120/71‑1 (MQ=255)                  cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc                                                               >  1:4718778/1‑53 (MQ=255)                  cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt                                                     >  1:644829/1‑63 (MQ=255)                  cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                             >  1:4132303/1‑71 (MQ=255)                  cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                             >  1:4187979/1‑71 (MQ=255)                  cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                             >  1:13892/1‑71 (MQ=255)                   ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                                      <  1:440645/61‑1 (MQ=255)                   ggCACATCGCAGTTTTCCAGCAGGGCATAAATAGGCTGTAACGCCATTTTACt                                                              <  1:3241060/53‑1 (MQ=255)                    gCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACg                                           <  1:1606902/71‑1 (MQ=255)                      acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                         <  1:4686244/71‑1 (MQ=255)                        aTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt                                                              <  1:4270545/48‑1 (MQ=255)                        aTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt                                                              <  1:4264784/48‑1 (MQ=255)                           tcAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc                                                               >  1:146231/2‑44 (MQ=255)                           gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt                                                                 >  1:1539310/1‑42 (MQ=255)                           gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc                                                               >  1:2339214/1‑44 (MQ=255)                           gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc                                                               >  1:2031202/1‑44 (MQ=255)                           gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc                                                               >  1:4331642/1‑44 (MQ=255)                           gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                             >  1:4710180/1‑62 (MQ=255)                           gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                             >  1:5032353/1‑62 (MQ=255)                            cAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc                                   <  1:5069817/71‑1 (MQ=255)                             aGTTTTCCAGCAGGTCATATATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg                                                  <  1:1726036/55‑1 (MQ=255)                              gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt                                                              <  1:4219566/42‑1 (MQ=255)                              gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt                                                              <  1:4940639/42‑1 (MQ=255)                               ttttCCAGCAGGTCATAAATAGGCTGTAACGCCatttt                                                                 <  1:2718534/38‑1 (MQ=255)                               ttttCCAGCAGGTCATAAATAGGCTGTAACGCCatttt                                                                 <  1:5045399/38‑1 (MQ=255)                               ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac                                            <  1:3974134/59‑1 (MQ=255)                               ttttCCAGCAGGTCATAAATAGGCTGTAACACCATTTTACTGTCGCCCATGTGGAacac                                            <  1:3107805/59‑1 (MQ=255)                                 ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc                                                        >  1:362692/1‑45 (MQ=255)                                 ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt                              >  1:1442658/1‑71 (MQ=255)                                   ccAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGttt                             <  1:333680/70‑1 (MQ=255)                                   ccAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGttt                             <  1:5048555/70‑1 (MQ=255)                                    cagcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc                           >  1:630112/1‑71 (MQ=255)                                        agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa                                               >  1:738867/1‑47 (MQ=255)                                        agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa                                               >  1:1167588/1‑47 (MQ=255)                                          gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                         <  1:4323377/51‑1 (MQ=255)                                          gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                         <  1:2003569/51‑1 (MQ=255)                                            cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGa                                                >  1:926099/1‑42 (MQ=255)                                            cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg                    >  1:1550544/1‑70 (MQ=255)                                            cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg                    >  1:5010585/1‑70 (MQ=255)                                            cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg                    >  1:2042610/1‑70 (MQ=255)                                             atatatAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGc                   >  1:2124657/1‑70 (MQ=255)                                             aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGc                   <  1:1092548/70‑1 (MQ=255)                                              tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc                       <  1:1331499/65‑1 (MQ=255)                                               aaaTAGGCTGTAACGCCATTTTACTGTCGCCCAtg                                                    <  1:4670801/35‑1 (MQ=255)                                               aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa                >  1:684631/1‑71 (MQ=255)                                                aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa                >  1:1242590/1‑70 (MQ=255)                                                aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa                <  1:1472584/70‑1 (MQ=255)                                                aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa                <  1:2716664/70‑1 (MQ=255)                                                  tAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCg             >  1:1899219/1‑71 (MQ=255)                                                       tGTAACGCCATTTTACTGTCGCCCATGTGGAACACg                                           <  1:4111755/36‑1 (MQ=255)                                                       tGTAACGCCATTTTACTGTCGCCCATGTGGAACACg                                           <  1:3178710/36‑1 (MQ=255)                                                       tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAc        >  1:1792482/1‑71 (MQ=255)                                                         tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa                >  1:3267798/1‑61 (MQ=255)                                                         tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa                >  1:886192/1‑61 (MQ=255)                                                         tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcg       <  1:3698123/70‑1 (MQ=255)                                                         tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGACAGGTTTACCGCCGAGCaaa                >  1:4897163/1‑61 (MQ=255)                                                          aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGa                                       >  1:2115587/1‑37 (MQ=255)                                                          aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGa                                       >  1:3333294/1‑37 (MQ=255)                                                          aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg     >  1:4988118/1‑71 (MQ=255)                                                          aaCGCCATTATACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg     >  1:1778643/1‑71 (MQ=255)                                                           aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGAc                                      >  1:959862/1‑37 (MQ=255)                                                             gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGa                     >  1:2369320/1‑52 (MQ=255)                                                             gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGa  <  1:4505697/71‑1 (MQ=255)                                                             gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGa  <  1:3456757/71‑1 (MQ=255)                                                             gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGa  <  1:3544326/71‑1 (MQ=255)                                                                |                                                                    GGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGA  >  W3110S.gb/4563526‑4563656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑