| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,580,803 | C→T | 30.4% | pseudogene (1987/2721 nt) | yjiV → | ECK4334:JW5954:b4486; conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,580,803 | 0 | C | T | 30.4% | 69.5 / 30.5 | 46 | pseudogene (1987/2721 nt) | yjiV | ECK4334:JW5954:b4486; conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (15/17); new base T (4/10); total (19/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAATGGGGGTTGATACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACTGAACGA > W3110S.gb/4580735‑4580870 | aaaTGGGGGTTGATACCGATCCCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCg < 1:4715790/71‑1 (MQ=255) aaTGGGGGTTGATACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCgg < 1:5133583/71‑1 (MQ=255) ggTTGATACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGc < 1:2839193/71‑1 (MQ=255) ggTTGATACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGc < 1:488591/71‑1 (MQ=255) gTTGATACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCgg < 1:2319301/64‑1 (MQ=255) ttGATACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCgg < 1:111938/63‑1 (MQ=255) aTACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTg < 1:553943/71‑1 (MQ=255) aCCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGaa > 1:2954933/1‑71 (MQ=255) ccGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGt > 1:1735183/1‑60 (MQ=255) tgatgaCGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCg < 1:4458993/47‑1 (MQ=255) tgatgaCGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCg < 1:1641480/47‑1 (MQ=255) tgatgaCGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCa < 1:4823887/55‑1 (MQ=255) tgatgaCGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACtg < 1:3578196/71‑1 (MQ=255) tgaCGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACtggtg < 1:829997/71‑1 (MQ=255) gaCGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCAGCTGAATGACGAACt > 1:2542069/1‑66 (MQ=255) cGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCACCTGAATGACGAACTGgtggtg < 1:4105810/71‑1 (MQ=255) cGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGCGGCACCTGAATGACGAACTGgtggtg < 1:3587083/71‑1 (MQ=255) tGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCgg < 1:2099593/39‑1 (MQ=255) gCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTa < 1:2635969/70‑1 (MQ=255) gCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTa < 1:4181229/70‑1 (MQ=255) gCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTa < 1:3596162/70‑1 (MQ=255) cGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCgg > 1:4483438/1‑71 (MQ=255) cagccagcAGGCAACGATTCCGGGGCCGGAGTGGCACCTGaa > 1:5088491/1‑42 (MQ=255) gccagcAGGCAACGATTCCGGGGTCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTtat < 1:3727055/71‑1 (MQ=255) ccagcAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTtata < 1:2680901/71‑1 (MQ=255) gcagTCAACGATTCCGGGGTCGGAGTGGCACCTGAATGACGAACtgg < 1:2405277/47‑1 (MQ=255) gcagGCAACGATTCCGGGGTCGGAGTGGCACCTGAATGACGAACtgg < 1:2411170/47‑1 (MQ=255) cagGCAACGATTCCGGGGTCGGAGTGGCACCTGAATGACGAACTGgtggtg < 1:423347/51‑1 (MQ=255) cAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACtggt < 1:3274631/43‑1 (MQ=255) cAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTa > 1:2177601/1‑51 (MQ=255) aaCGATTCCGGTGTCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACgg > 1:3320680/1‑68 (MQ=255) aaCGATTCCGGGGCCGGAGTGGCACCTGAATGACGAAc > 1:3454276/1‑38 (MQ=255) aaCGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTa > 1:3530094/1‑50 (MQ=255) aaCGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTa > 1:4561791/1‑50 (MQ=255) aaCGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTa > 1:2549984/1‑50 (MQ=255) aaCGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTta > 1:1285593/1‑60 (MQ=255) aaCGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACgg > 1:3111837/1‑68 (MQ=255) gATTCCGGGGTCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAAc > 1:3438535/1‑71 (MQ=255) gATTCCGGGGTCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAAc > 1:705229/1‑71 (MQ=255) gATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAAc > 1:4752427/1‑71 (MQ=255) gATTCAGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAAc > 1:510482/1‑71 (MQ=255) tCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGtc > 1:4694576/1‑47 (MQ=255) ccGGGGTCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTtata > 1:2727467/1‑55 (MQ=255) cGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACTGAAc < 1:1844891/71‑1 (MQ=255) gggCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACTGAACGa < 1:4920980/71‑1 (MQ=255) ggCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACTGAACGa < 1:252099/70‑1 (MQ=255) | AAATGGGGGTTGATACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACTGAACGA > W3110S.gb/4580735‑4580870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |