| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,604,824 | G→A | 13.5% | S16S (AGC→AGT) | yjjA ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,604,824 | 0 | G | A | 13.5% | 251.3 / 24.5 | 89 | S16S (AGC→AGT) | yjjA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (35/42); new base A (5/7); total (40/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTTCGCTGGCGGCGCTGGAGAGCGCATCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTG > W3110S.gb/4604756‑4604891 | aGTTCGCTGGCGGCGCTGGAGAGCGCATCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATgcgc > 1:381067/1‑70 (MQ=255)aGTTCGCTGGCGGCGCTGGAGAGCGCATCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCt > 1:934447/1‑71 (MQ=255)aGTTCGCTGGCGGCGCTGGAGAGCGCATCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCt > 1:2487113/1‑71 (MQ=255)aGTTCGCTGGCGGCGCTGGAGAGCGCATCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCt > 1:2498276/1‑71 (MQ=255)aGTTCGCTGGCGGCGCTGGAGAGCGCATCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCt > 1:3384439/1‑71 (MQ=255)aGTTCGCTGGCGGCGCTGGAGAGCGCATCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCt > 1:2733555/1‑71 (MQ=255) cTGGCGGCGCTGGAGAGCGCATCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTggcggc < 1:2966709/71‑1 (MQ=255) tGGCGGCGCTGGAGAGCGCATCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTggcggc < 1:500497/70‑1 (MQ=255) gtcgcTGGAGAGCGCATCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATgg < 1:3175610/69‑1 (MQ=255) cgcATCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCACTGGCGGCAATGGCACAGCACagaa < 1:3611174/71‑1 (MQ=255) tCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTggcggc < 1:2119771/50‑1 (MQ=255) tttCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGtt > 1:1411196/1‑71 (MQ=255) tttCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGtt > 1:4866574/1‑71 (MQ=255) tCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGttt > 1:4875203/1‑70 (MQ=255) ccAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGtt < 1:696371/68‑1 (MQ=255) cAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTTGCGGCAATGGCACAGCACAGAAGATGTTTaa < 1:3744001/70‑1 (MQ=255) cAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTaa < 1:411709/70‑1 (MQ=255) cAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTaa < 1:2019384/70‑1 (MQ=255) ggACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAg < 1:2508431/71‑1 (MQ=255) gACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGt < 1:3282122/71‑1 (MQ=255) gACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGt > 1:5064728/1‑71 (MQ=255) gACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGt > 1:3393373/1‑71 (MQ=255) gACGCAGCATGCACCCCGGTGGAAATTAATGCACTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGt > 1:1362024/1‑71 (MQ=255) gcagcaTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGtttt > 1:1606430/1‑71 (MQ=255) gcagcaTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGttt > 1:2862587/1‑70 (MQ=255) cagcaTGCACCCCGGTGGAAATTAATGCACTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtc < 1:4872986/71‑1 (MQ=255) agcaTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtca > 1:3361746/1‑71 (MQ=255) agcaTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtca > 1:2276154/1‑71 (MQ=255) agcaTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtca > 1:3033666/1‑71 (MQ=255) caTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtcatc < 1:3650808/71‑1 (MQ=255) caTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtcat > 1:3035468/1‑70 (MQ=255) tGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtcatcat < 1:1229090/71‑1 (MQ=255) tGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtcatcat < 1:4598664/71‑1 (MQ=255) tGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtcatcat < 1:4111211/71‑1 (MQ=255) tGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtcatcat < 1:3835069/71‑1 (MQ=255) tGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtcatcat < 1:2334461/71‑1 (MQ=255) tGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtcatcat < 1:3413175/71‑1 (MQ=255) gCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtcatcat > 1:3160029/1‑70 (MQ=255) cACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtcatcatca > 1:3095925/1‑71 (MQ=255) cACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtcatcatca > 1:4715919/1‑71 (MQ=255) cACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtcatcatca > 1:4721648/1‑71 (MQ=255) cccGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGtt < 1:3009346/58‑1 (MQ=255) cccGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGtt < 1:3952680/58‑1 (MQ=255) cccGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGtt < 1:2222467/58‑1 (MQ=255) cccGGTGGAAATTAATGCACTGGCGGCAATGGCACAGCACAGAAGATGTTTaa < 1:1897887/53‑1 (MQ=255) cccGGTGGAAATTAATGCACTGGCGGCAATGGCACAGCACAGAAGATGTTTaa < 1:4630828/53‑1 (MQ=255) ccGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACa > 1:2809302/1‑54 (MQ=255) ccGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACa > 1:4681674/1‑54 (MQ=255) ccGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACa > 1:366386/1‑54 (MQ=255) ccGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACa > 1:5042696/1‑54 (MQ=255) ccGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTAc > 1:4133627/1‑71 (MQ=255) ccGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTAc > 1:4300237/1‑71 (MQ=255) ccGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTAc > 1:1271866/1‑71 (MQ=255) cGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACagaa < 1:4686469/42‑1 (MQ=255) cGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGtt < 1:2623994/56‑1 (MQ=255) cGGTGGAAATTAATGCACTGGCGGCAATGGCACAGCACAGAAGATGTTTAACa > 1:2537951/1‑53 (MQ=255) cGGTGGAAATTAATGCACTGGCGGCAATGGCACAGCACAGAAGATGTTTAACa > 1:3409856/1‑53 (MQ=255) cGGTGGAAATTAATGCACTGGCGGCAATGGCACAGCACAGAAGATGTTTAACa > 1:4147154/1‑53 (MQ=255) cGGTGGAAATTAATGCACTGGCGGCAATGGCACAGCACAGAAGATGTTTAACa > 1:1677385/1‑53 (MQ=255) ggTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACa < 1:3639544/52‑1 (MQ=255) gTGGAAATTAATTCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCa < 1:835221/71‑1 (MQ=255) gTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGttt < 1:1919490/47‑1 (MQ=255) gTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCa < 1:766292/71‑1 (MQ=255) gTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCa < 1:416726/71‑1 (MQ=255) gTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCa < 1:2989601/71‑1 (MQ=255) tGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCaa < 1:4916977/71‑1 (MQ=255) tGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCaa < 1:4432939/71‑1 (MQ=255) tGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCaa < 1:4508629/71‑1 (MQ=255) tGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCaa < 1:2043002/71‑1 (MQ=255) tGGAAATTAATGCACTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAg < 1:4743339/51‑1 (MQ=255) tGGAAATTAATGCACTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAg < 1:3789866/51‑1 (MQ=255) ggAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtcat > 1:3759408/1‑57 (MQ=255) gAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtcatcat < 1:988466/59‑1 (MQ=255) gAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAgg > 1:5138400/1‑71 (MQ=255) aaaTTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtc < 1:759714/53‑1 (MQ=255) aaaTTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTtc < 1:2075341/53‑1 (MQ=255) aaTTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTg < 1:1184046/71‑1 (MQ=255) aaTTAATGCGCTGGCGGAAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTg < 1:2043603/71‑1 (MQ=255) aTTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAg > 1:4283266/1‑46 (MQ=255) aTTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCaa < 1:2927845/66‑1 (MQ=255) aTTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCaa < 1:1407242/66‑1 (MQ=255) aTTAATGCACTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTgg < 1:700657/71‑1 (MQ=255) ttAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTgg > 1:1069946/1‑70 (MQ=255) ttAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTgg > 1:4715012/1‑70 (MQ=255) ttAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGa < 1:937025/71‑1 (MQ=255) tAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGaa > 1:4742944/1‑71 (MQ=255) aaTGCGCTTGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAAt > 1:2817666/1‑71 (MQ=255) aaTGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTa > 1:2557363/1‑58 (MQ=255) aTGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGTAtt < 1:2624951/71‑1 (MQ=255) tGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATtg < 1:1969454/71‑1 (MQ=255) | AGTTCGCTGGCGGCGCTGGAGAGCGCATCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTG > W3110S.gb/4604756‑4604891 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |