| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,606,089 | G→A | 31.4% | N37N (AAC→AAT) | dnaT ← | DNA biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,606,089 | 0 | G | A | 31.4% | 45.0 / 27.1 | 35 | N37N (AAC→AAT) | dnaT | DNA biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base G (10/14); new base A (8/3); total (18/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01 | |||||||||||
TTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCA > W3110S.gb/4606025‑4606153 | tttCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGc > 1:621312/1‑70 (MQ=255) ttcCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATa > 1:3100613/1‑71 (MQ=255) ccAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATa < 1:68287/69‑1 (MQ=255) gcgcCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGc > 1:4369005/1‑70 (MQ=255) ttCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac < 1:4000847/66‑1 (MQ=255) cAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCt > 1:3138275/1‑71 (MQ=255) aGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGt < 1:1497249/44‑1 (MQ=255) caggcgtgcaggcgtgACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCa < 1:2916381/70‑1 (MQ=255) gcgtgcaggcgtgACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCtt > 1:3306844/1‑71 (MQ=255) caggcgtgACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTg > 1:4537224/1‑69 (MQ=255) aggcgtgACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGc > 1:3188832/1‑69 (MQ=255) ggcgtgACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAg < 1:4648567/71‑1 (MQ=255) cgtgACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGaa < 1:1707522/71‑1 (MQ=255) aCGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACca > 1:2233019/1‑43 (MQ=255) ggCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGttt < 1:4767357/71‑1 (MQ=255) aTAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTgg > 1:4285415/1‑70 (MQ=255) aTAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGt > 1:352960/1‑71 (MQ=255) tAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCtttt < 1:1732629/54‑1 (MQ=255) tAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGt > 1:3391847/1‑70 (MQ=255) tAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTg < 1:3941174/71‑1 (MQ=255) aaaCGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAg > 1:2416625/1‑47 (MQ=255) aaaCGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGa < 1:3595993/70‑1 (MQ=255) aaaCGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGa < 1:2525791/70‑1 (MQ=255) aaaCGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGAt > 1:340589/1‑71 (MQ=255) aaaCGCCGGGGCATTGTTAGCAAATACGGCAACAACACCGCCTTCAg > 1:299416/1‑47 (MQ=255) aaaCGCCGGGGCATTATTAGCAAATACGGCAACcaca > 1:3062823/1‑37 (MQ=255) aaaCGCCGGGGCATTATTAGCAAATACGGCAACca > 1:574671/1‑35 (MQ=255) aaaCGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCGGAACGGTTTGGTGa > 1:4674596/1‑70 (MQ=255) aCGCCGGGGCATTGTTAGCAAATACGGCAACCacac > 1:5085359/1‑36 (MQ=255) aCGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCg < 1:4054864/71‑1 (MQ=255) aCGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCg < 1:3818375/71‑1 (MQ=255) ccGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTAAGCTTTTGCCAGAACGGTTTGGTGATCgtgt < 1:2941646/71‑1 (MQ=255) ggCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCa < 1:4777973/71‑1 (MQ=255) ttGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAg < 1:4050938/44‑1 (MQ=255) ttGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAg < 1:1342836/44‑1 (MQ=255) | TTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCA > W3110S.gb/4606025‑4606153 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |