| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 406,395 | C→T | 28.1% | intergenic (+1/‑257) | yaiA → / → aroM | hypothetical protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 406,395 | 0 | C | T | 28.1% | 93.1 / 40.9 | 57 | intergenic (+1/‑257) | yaiA/aroM | hypothetical protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (22/19); new base T (7/9); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
GAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTC > W3110S.gb/406329‑406464 | gAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCa > 1:2230138/1‑71 (MQ=255) cATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCAt > 1:1496570/1‑69 (MQ=255) cATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCAt > 1:3335679/1‑69 (MQ=255) ccGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGATCGCATCAGAc > 1:4268905/1‑71 (MQ=255) cGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACt > 1:4996319/1‑71 (MQ=255) gCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCg < 1:4529339/71‑1 (MQ=255) cTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCgg < 1:4871632/71‑1 (MQ=255) cTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCgg < 1:2887327/71‑1 (MQ=255) cTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCgg < 1:2163211/71‑1 (MQ=255) cTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCgg < 1:1858293/71‑1 (MQ=255) cTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCg > 1:2426589/1‑70 (MQ=255) cTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCACACTGCTCgg < 1:450169/71‑1 (MQ=255) ggAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACAGCATCAGACTGCTCGGaa > 1:2649339/1‑69 (MQ=255) gAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCa < 1:2877218/56‑1 (MQ=255) gAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAgg > 1:4551520/1‑70 (MQ=255) aGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGa > 1:4148489/1‑56 (MQ=255) gatggatgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGATCGCATCAGACTGCTCGGAAGGGATTCt > 1:4305868/1‑71 (MQ=255) gatggatgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGATCGCATCAGACTGCTCGGAAGGGATTCt > 1:735928/1‑71 (MQ=255) gatggatgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGa > 1:4155490/1‑53 (MQ=255) atggatgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTg < 1:3665002/71‑1 (MQ=255) atggatgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTg < 1:294938/71‑1 (MQ=255) tggatgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGATCGCATCAGACTGCTCGGAAGGGATTCt > 1:3391006/1‑69 (MQ=255) tggatgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGa > 1:1578619/1‑65 (MQ=255) gatgCGAAAAAACTCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGt < 1:796828/71‑1 (MQ=255) tgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGATCGCATCAGACTGCTCGGAAGGGATTCTg < 1:4638354/66‑1 (MQ=255) gCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGAt < 1:3881648/61‑1 (MQ=255) cGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCa > 1:323977/1‑71 (MQ=255) gAAAAAACGCTATGAGGACCCTGACAAAGAGTGATCGCATCAGACTGCTCgg < 1:4703070/52‑1 (MQ=255) gAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCAc > 1:4223808/1‑71 (MQ=255) aaaaaaCGCTATGAGGACCCTGACAAAGAGTGATCGCATCAGACTGCTCGGAAGGGATTCt < 1:1898985/61‑1 (MQ=255) cGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTTAGTGCCACTACAAgg > 1:1113661/1‑71 (MQ=255) cGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAgg > 1:4994520/1‑71 (MQ=255) gCTATGAGGACCCTGACAAAGAGTGATCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAggg < 1:5004918/71‑1 (MQ=255) gCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAgg > 1:2614577/1‑70 (MQ=255) tATGAGGACCCTGACAAAGAGTGATCGCATCAGACTGCTCGGaa < 1:4762953/44‑1 (MQ=255) tATGAGGACCCTGACAAAGAGTGATCGCATCAGACTGCTCGGaa < 1:3457249/44‑1 (MQ=255) aGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGc > 1:4754137/1‑71 (MQ=255) aGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGc > 1:1759449/1‑71 (MQ=255) ggACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCg < 1:560433/71‑1 (MQ=255) cTGACAAAGAGTGATCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTAc < 1:1669769/54‑1 (MQ=255) cTGACAAAGAGTGACCGCATCAGACTGCTCGGAAgg < 1:3049101/36‑1 (MQ=255) cTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTcaca > 1:3124444/1‑71 (MQ=255) tGACAAAGAGTGATCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACAt > 1:218751/1‑71 (MQ=255) tGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACAt > 1:507782/1‑71 (MQ=255) gACAAAGAGTGATCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACt < 1:2996264/50‑1 (MQ=255) gACAAAGAGTGATCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACAtt > 1:1394184/1‑71 (MQ=255) gACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACAtt > 1:3968564/1‑71 (MQ=255) cAAAGAGTGATCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACaa > 1:1743630/1‑52 (MQ=255) aaaGAGTGATCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACAttttt < 1:44460/71‑1 (MQ=255) aaaGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTg > 1:1253731/1‑38 (MQ=255) aaGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTc < 1:4289607/71‑1 (MQ=255) agagTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCa < 1:1782525/71‑1 (MQ=255) agagTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCa < 1:229089/71‑1 (MQ=255) gagTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCAt < 1:1909039/71‑1 (MQ=255) gagTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCAt < 1:1475025/71‑1 (MQ=255) aCCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAAtt > 1:369301/1‑70 (MQ=255) aCCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTc < 1:1822057/71‑1 (MQ=255) | GAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTC > W3110S.gb/406329‑406464 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |