| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 429,850 | T→C | 47.2% | Y8H (TAT→CAT) | yajD → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 429,850 | 0 | T | C | 47.2% | 1.8 / 75.6 | 53 | Y8H (TAT→CAT) | yajD | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (14/14); new base C (15/10); total (29/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGC > W3110S.gb/429793‑429915 | tGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAg > 1:1870039/1‑70 (MQ=255) cgcgTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGa < 1:4316799/69‑1 (MQ=255) cgcgTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGaa < 1:2828678/70‑1 (MQ=255) gcgTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAg < 1:4234895/71‑1 (MQ=255) aCATTTCATCCAGGTAACTTTTCATCGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATcgc > 1:1679996/1‑71 (MQ=255) cATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATcgc > 1:807436/1‑70 (MQ=255) aTTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGaa < 1:951937/57‑1 (MQ=255) ttCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGaa > 1:3070266/1‑70 (MQ=255) tCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGaaa > 1:2512757/1‑70 (MQ=255) aTCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAg < 1:4974266/71‑1 (MQ=255) cAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCAATCGCGAAAAAGCa > 1:1344234/1‑70 (MQ=255) ggTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGt > 1:4718356/1‑42 (MQ=255) gTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCa < 1:1874492/67‑1 (MQ=255) gTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCa < 1:4790585/67‑1 (MQ=255) gTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAg < 1:711315/44‑1 (MQ=255) gTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTa > 1:1174988/1‑70 (MQ=255) gTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTa > 1:3558196/1‑70 (MQ=255) tAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaaa < 1:3068294/71‑1 (MQ=255) aaCTTTTCATGGCTATGATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaaa > 1:2224367/1‑70 (MQ=255) aaCTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGaa > 1:4541843/1‑44 (MQ=255) aaCTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGaa > 1:881925/1‑44 (MQ=255) aaCTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGaa > 1:115530/1‑44 (MQ=255) aaCTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGaa > 1:1967657/1‑44 (MQ=255) aaCTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGaa > 1:2118556/1‑44 (MQ=255) aaCTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaaa > 1:4476173/1‑70 (MQ=255) aCTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaaaa < 1:515112/70‑1 (MQ=255) aCTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaaaa < 1:1494255/70‑1 (MQ=255) aCTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaaaa < 1:3755299/70‑1 (MQ=255) cTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAAt > 1:1373591/1‑71 (MQ=255) ttttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATc > 1:2492415/1‑71 (MQ=255) ttttCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAAt > 1:2373161/1‑70 (MQ=255) tttCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGaa > 1:4960112/1‑40 (MQ=255) ttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGaaa > 1:3331134/1‑55 (MQ=255) ttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGaa > 1:1005188/1‑54 (MQ=255) tGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAg < 1:3444037/54‑1 (MQ=255) tGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCg < 1:388764/71‑1 (MQ=255) ggCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGt > 1:2052414/1‑71 (MQ=255) cTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTgg < 1:2541812/71‑1 (MQ=255) tATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCt < 1:2436813/38‑1 (MQ=255) atcatcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTggg > 1:697258/1‑70 (MQ=255) catcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCt > 1:4070478/1‑71 (MQ=255) atcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATc < 1:3733130/61‑1 (MQ=255) atcCCAAAAAACCAT‑‑GCGGTTAGAAAGCGGCTATCGCGAAAAAg > 1:2200935/1‑44 (MQ=37) tcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaaa < 1:832712/52‑1 (MQ=255) tcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaaa < 1:4343022/52‑1 (MQ=255) cccAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCg > 1:4645064/1‑61 (MQ=255) cAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaaaa < 1:1863127/50‑1 (MQ=255) cAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaaaa < 1:1343516/50‑1 (MQ=255) aaaaaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTc < 1:2567007/70‑1 (MQ=255) aaaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCAtt > 1:2953596/1‑44 (MQ=255) aaaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTgg > 1:4579098/1‑60 (MQ=255) aaaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCt < 1:3901221/64‑1 (MQ=255) aaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGc < 1:4160786/71‑1 (MQ=255) | TGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGC > W3110S.gb/429793‑429915 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |