| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 459,715 | A→G | 20.5% | Y535C (TAC→TGC) | lon → | DNA‑binding ATP‑dependent protease La |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 459,715 | 0 | A | G | 20.5% | 156.1 / 35.4 | 73 | Y535C (TAC→TGC) | lon | DNA‑binding ATP‑dependent protease La |
| Reads supporting (aligned to +/- strand): ref base A (22/36); new base G (11/4); total (33/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.98e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGC > W3110S.gb/459649‑459783 | gTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACAcc < 1:299734/71‑1 (MQ=255) aTGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGt < 1:2716624/71‑1 (MQ=255) aTGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGt < 1:1864216/71‑1 (MQ=255) tGCACTGAAATAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGt < 1:5113762/70‑1 (MQ=255) gCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGt > 1:1016618/1‑69 (MQ=255) cACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAg < 1:3293260/71‑1 (MQ=255) cACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAg > 1:1603808/1‑71 (MQ=255) gAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAggcgg > 1:1576592/1‑71 (MQ=255) gAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAggcgg > 1:160986/1‑71 (MQ=255) aaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAGGCGGgcgtg > 1:4071010/1‑71 (MQ=255) aaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAGGCGGgcgtg > 1:5093061/1‑71 (MQ=255) aaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAGGCGGgcgt > 1:4470806/1‑70 (MQ=255) aaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgtg < 1:500304/71‑1 (MQ=255) ggTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgtgcg > 1:4172102/1‑71 (MQ=255) gTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGt < 1:2702622/56‑1 (MQ=255) gAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTgg > 1:4844241/1‑71 (MQ=255) gCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTc > 1:4741813/1‑71 (MQ=255) tGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGa > 1:2960865/1‑52 (MQ=255) gACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCg > 1:4622040/1‑48 (MQ=255) gACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAGGCGGGcgtgcgtg > 1:444616/1‑65 (MQ=255) gACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAgg < 1:4812490/53‑1 (MQ=255) gACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTg > 1:3121779/1‑70 (MQ=255) gACCGTCGACGATAGCACCATTATCGGCATTATTCGTTACTACACCCGTGAgg < 1:4750450/53‑1 (MQ=255) aCCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTg < 1:3367964/69‑1 (MQ=255) aCCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTg < 1:334748/69‑1 (MQ=255) aCCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTg < 1:3169056/69‑1 (MQ=255) tCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGt < 1:563268/44‑1 (MQ=255) tCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGt < 1:915536/44‑1 (MQ=255) gacgaTAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGa > 1:1794506/1‑44 (MQ=255) gacgaTAGCGCAATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGa < 1:722837/71‑1 (MQ=255) acgaTAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGaa < 1:4048525/71‑1 (MQ=255) cgaTAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGaaa < 1:4700493/71‑1 (MQ=255) gaTAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAAt > 1:519217/1‑71 (MQ=255) gaTAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAAt > 1:3470469/1‑71 (MQ=255) aTAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGa < 1:3036997/40‑1 (MQ=255) aTAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGa < 1:2541915/40‑1 (MQ=255) aTAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGa < 1:376931/40‑1 (MQ=255) aTAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTgg < 1:136333/55‑1 (MQ=255) aGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTc > 1:3021625/1‑55 (MQ=255) aGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAAtctc > 1:978217/1‑71 (MQ=255) gcgcCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTg < 1:3515686/56‑1 (MQ=255) gcgcCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTg < 1:3918432/63‑1 (MQ=255) gcgcCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTg < 1:553538/63‑1 (MQ=255) gcCATTATCGGCATTATTCGTTACTGCACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCaa < 1:2698184/71‑1 (MQ=255) aTTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACt > 1:3255246/1‑71 (MQ=255) aTTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACt < 1:1151184/71‑1 (MQ=255) aTTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACt < 1:1369052/71‑1 (MQ=255) aTTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACt > 1:5074286/1‑71 (MQ=255) ttATCGGCATTATTCGTTACTGCACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCa < 1:544198/66‑1 (MQ=255) ttATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTgg < 1:987838/46‑1 (MQ=255) ttATCGGCATTATTCGTTACTACACCCGTGAGGAGGGCGTGCGTgg < 1:1485436/46‑1 (MQ=255) tATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACtgt > 1:3621971/1‑71 (MQ=255) aTCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgtgcgt > 1:3500171/1‑42 (MQ=255) aTCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTc > 1:4559074/1‑46 (MQ=255) aTCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTc > 1:1394115/1‑46 (MQ=255) tCGGCATTATTCGTTACTGCACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTgtgt < 1:2977291/71‑1 (MQ=255) tCGGCATTATTCGTTACTACACCCGTGAggcgggc < 1:2778918/35‑1 (MQ=255) cGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCaaa > 1:1089587/1‑64 (MQ=255) gCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGc < 1:4943717/71‑1 (MQ=255) attattCGTTACTGCACCCGTGAGGCGGgcgtgcgt > 1:676334/1‑36 (MQ=255) attattCGTTACTGCACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCa > 1:854109/1‑70 (MQ=255) ttattCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGc < 1:3339407/68‑1 (MQ=255) ttattCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGc < 1:1943436/68‑1 (MQ=255) ttattCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCaaa < 1:567883/71‑1 (MQ=255) ttattCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCaaa < 1:4118705/71‑1 (MQ=255) ttattCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCaaa < 1:1511377/71‑1 (MQ=255) tattCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGaa < 1:2431395/49‑1 (MQ=255) tattCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGaa < 1:1266539/49‑1 (MQ=255) attCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAAt > 1:1601287/1‑50 (MQ=255) attCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAAt > 1:67338/1‑50 (MQ=255) attCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCaaa > 1:2574532/1‑69 (MQ=255) attCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCaa > 1:2625450/1‑68 (MQ=255) ttCGTTACTGCACCCGTGAGGCGGGCGTGCGTGGTGTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCg > 1:2485695/1‑71 (MQ=255) tactacACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGtt > 1:3494861/1‑69 (MQ=255) actgcACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAg > 1:2706301/1‑71 (MQ=255) ctacACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGc < 1:907523/71‑1 (MQ=255) | GTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGC > W3110S.gb/459649‑459783 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |