| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 460,284 | G→T | 28.2% | A725S (GCA→TCA) | lon → | DNA‑binding ATP‑dependent protease La |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 460,284 | 0 | G | T | 28.2% | 90.7 / 19.9 | 39 | A725S (GCA→TCA) | lon | DNA‑binding ATP‑dependent protease La |
| Reads supporting (aligned to +/- strand): ref base G (15/13); new base T (5/6); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
AATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGA > W3110S.gb/460217‑460348 | aaTGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAg > 1:3456859/1‑71 (MQ=255) ctctGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATcgc < 1:4893941/62‑1 (MQ=255) tctGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAgcgc < 1:4891845/56‑1 (MQ=255) tctGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCgcgg > 1:922959/1‑63 (MQ=255) ctGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCgcgg > 1:3737840/1‑62 (MQ=255) ctGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCgcgg > 1:2012005/1‑62 (MQ=255) ctGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTa > 1:342181/1‑70 (MQ=255) cGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGTCAGCGCATCGCGGCGGGATTaaaa < 1:3371227/70‑1 (MQ=255) gTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGTCAGCGCATCGCGGCGGGATTAAAACAGt < 1:1604257/70‑1 (MQ=255) aGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTaa > 1:3355987/1‑61 (MQ=255) cTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGTCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTcc > 1:2129965/1‑71 (MQ=255) cTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGTCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTcc > 1:3000488/1‑71 (MQ=255) gCCGATCGGTGGTTTGAAAGAAAAACTCCTGTCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGt > 1:3355774/1‑71 (MQ=255) ccGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGtt > 1:2653575/1‑71 (MQ=255) ccGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGtt > 1:3440684/1‑71 (MQ=255) aTCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATcgc < 1:2794313/39‑1 (MQ=255) ggtggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGa > 1:3830199/1‑43 (MQ=255) tggtTTGAAAGAAAAACTCCTGTCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATa < 1:531189/71‑1 (MQ=255) tggtTTGAAAGAAAAACTCCTGTCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATa < 1:2794255/71‑1 (MQ=255) tggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTaa < 1:1364034/45‑1 (MQ=255) ggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATa > 1:1075213/1‑70 (MQ=255) tttGAAAGAAAAACTCCTGGCAGCGCATCGcggcgg < 1:2690217/36‑1 (MQ=255) tttGAAAGAAAAACTCCTGGCAGCGCATCGcggcgg < 1:1647966/36‑1 (MQ=255) ttGAAAGAAAAACTCCTGTCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAAc > 1:4523831/1‑70 (MQ=255) tGAAAGAAAAACTCCTGTCAGCGCATCGCGGCGGGATTAAAACAGTGCt < 1:4657831/49‑1 (MQ=255) tGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAAcgc > 1:3502901/1‑71 (MQ=255) tGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAAcgc > 1:2714958/1‑71 (MQ=255) gaaagaaaAACTCCTGTCAGCGCATCGCGGCGGGATTaaaa < 1:4069815/41‑1 (MQ=255) gaaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTaa < 1:4796835/39‑1 (MQ=255) aaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAg > 1:3760302/1‑43 (MQ=255) aaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGa > 1:3583879/1‑71 (MQ=255) aagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTaaaa < 1:814093/39‑1 (MQ=255) aagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTaaaa < 1:1705121/39‑1 (MQ=255) aagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTaa < 1:3389364/37‑1 (MQ=255) agaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATc > 1:1046063/1‑71 (MQ=255) gaaaAACTCCTGTCAGCGCATCGCGGCGGGATTaaaa > 1:776515/1‑37 (MQ=255) gaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCt < 1:2265084/44‑1 (MQ=255) cTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAaga < 1:294418/71‑1 (MQ=255) tCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGaa < 1:579285/50‑1 (MQ=255) | AATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGA > W3110S.gb/460217‑460348 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |