| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 464,946 | A→G | 34.1% | F531L (TTC→CTC) | ybaE ← | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 464,946 | 0 | A | G | 34.1% | 38.1 / 38.4 | 41 | F531L (TTC→CTC) | ybaE | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (12/15); new base G (8/6); total (20/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.22e-01 | |||||||||||
ACCAGCCGCGCGGTGTCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGTTTTCT > W3110S.gb/464877‑465016 | aCCAGCCGCGCGGTGTCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGaa > 1:675115/1‑70 (MQ=255) gCCGCGCGGTGTCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAgcg > 1:721285/1‑71 (MQ=255) cgcgcgGTGTCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAgcggc > 1:1025417/1‑71 (MQ=255) gcgGTGTCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTc < 1:3561936/71‑1 (MQ=255) gcgGTGTCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGt > 1:160229/1‑70 (MQ=255) gCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCt > 1:3681670/1‑71 (MQ=255) ccGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCAt > 1:565963/1‑71 (MQ=255) ccGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCAt > 1:1293347/1‑71 (MQ=255) ttCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTaa > 1:4098264/1‑71 (MQ=255) tCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTc < 1:186623/56‑1 (MQ=255) cGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTc < 1:1804989/47‑1 (MQ=255) gCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc > 1:1566494/1‑57 (MQ=255) gCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc > 1:3159888/1‑57 (MQ=255) gCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc > 1:2663462/1‑57 (MQ=255) cGGGAGTGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGTCTa < 1:4608075/71‑1 (MQ=255) ggAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGc < 1:1271190/45‑1 (MQ=255) ggAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGc < 1:3359580/45‑1 (MQ=255) ggAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTTGCTaaa < 1:656068/71‑1 (MQ=255) ggAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaa < 1:1387529/71‑1 (MQ=255) gAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAg > 1:1375460/1‑43 (MQ=255) aGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaaaa > 1:1954426/1‑71 (MQ=255) gggCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGc < 1:297255/46‑1 (MQ=255) cTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCttt < 1:1532984/71‑1 (MQ=255) cTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaa < 1:1174603/62‑1 (MQ=255) cTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaa < 1:1197069/62‑1 (MQ=255) cTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaa < 1:4480148/62‑1 (MQ=255) cTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaa < 1:555259/62‑1 (MQ=255) aaTGCGATAGTGATAGTTGCGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCttt > 1:1837782/1‑69 (MQ=255) aaTGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCttt > 1:1877017/1‑69 (MQ=255) aaTGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCttt > 1:4652901/1‑69 (MQ=255) aaTGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCttt > 1:4776180/1‑69 (MQ=255) aTGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTc < 1:3486390/69‑1 (MQ=255) aTGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTc < 1:1432715/69‑1 (MQ=255) aTGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCa < 1:2947177/70‑1 (MQ=255) tGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaaa > 1:1128610/1‑59 (MQ=255) tGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCAGTCATTAAc > 1:3860712/1‑50 (MQ=255) cGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAggg > 1:2582775/1‑70 (MQ=255) cGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAggg > 1:429785/1‑70 (MQ=255) cGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAggg < 1:422225/70‑1 (MQ=255) atagtTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATc < 1:112908/71‑1 (MQ=255) gtTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCgg < 1:5056301/70‑1 (MQ=255) gAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGttt < 1:4665469/70‑1 (MQ=255) aCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAg > 1:1958167/1‑52 (MQ=255) aCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGTTttct < 1:4533826/71‑1 (MQ=255) | ACCAGCCGCGCGGTGTCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGTTTTCT > W3110S.gb/464877‑465016 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |