| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 478,343 | C→T | 47.4% | A45T (GCT→ACT) | ylaC ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 478,343 | 0 | C | T | 47.4% | 2.4 / 51.7 | 38 | A45T (GCT→ACT) | ylaC | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (6/14); new base T (5/13); total (11/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.89e-01 | |||||||||||
CAACCAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTC > W3110S.gb/478274‑478412 | cAACCAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGc < 1:1197904/70‑1 (MQ=255) aaCCAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGc < 1:3757719/69‑1 (MQ=255) ccAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACg < 1:2389856/71‑1 (MQ=255) cAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGt < 1:355067/71‑1 (MQ=255) aGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTa < 1:205817/71‑1 (MQ=255) aGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTa < 1:4822172/71‑1 (MQ=255) aGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACa < 1:2026268/70‑1 (MQ=255) cagaGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGAt > 1:759372/1‑70 (MQ=255) gTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCGACGTa < 1:3746817/47‑1 (MQ=255) tCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGtt > 1:4143341/1‑70 (MQ=255) tGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATa < 1:3106801/48‑1 (MQ=255) cAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTAAATACCGATAAACAGCCACGGATGTTTACGGAt < 1:337971/69‑1 (MQ=255) aCATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCgg < 1:2109662/55‑1 (MQ=255) aCATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATaaaa < 1:2737331/71‑1 (MQ=255) aCATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATaaa > 1:3919325/1‑70 (MQ=255) cATCACCGCCAGGGTGGCAAAAAAAGCAACGTACa < 1:3340856/35‑1 (MQ=255) tCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACt > 1:4476065/1‑70 (MQ=255) aGGGTGGAAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGtt < 1:5028612/49‑1 (MQ=255) ggTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGc < 1:4873021/71‑1 (MQ=255) ggTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGc < 1:1112600/71‑1 (MQ=255) ggTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGc < 1:1228430/71‑1 (MQ=255) tGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCgg > 1:1326144/1‑71 (MQ=255) gCAAAAAAAGTAACGTACATACCGATAAACAGCCACGGATGTTTACGGATAAAACTGATACTAAAGCgggg > 1:2008627/1‑71 (MQ=255) gCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATaaaa > 1:2786818/1‑54 (MQ=255) aaaaaaaGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGa > 1:2846488/1‑56 (MQ=255) aaaaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGtt < 1:3930007/71‑1 (MQ=255) aaaaaaGTAACGTACATACCGATAAACAGCCCCGGATGtt < 1:3415980/40‑1 (MQ=255) aaaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATaaaa > 1:990323/1‑51 (MQ=255) aaaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGAt > 1:3818881/1‑56 (MQ=255) aaaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGttt < 1:706424/71‑1 (MQ=255) aaaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGtt < 1:692527/70‑1 (MQ=255) aaaaaGTAACGTACATACCGATAAACAGCCCCGGATGtt < 1:1389420/39‑1 (MQ=255) aaaaaGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTttg < 1:3046823/71‑1 (MQ=255) aaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTttg < 1:3817972/71‑1 (MQ=255) aaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTttg < 1:1106562/71‑1 (MQ=255) aaaGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTttgtt < 1:2018263/71‑1 (MQ=255) aaaGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTttgtt < 1:3502288/71‑1 (MQ=255) aaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATaaaa > 1:2579406/1‑47 (MQ=255) aGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGTGTTtgttgt < 1:3620127/71‑1 (MQ=255) gCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTc < 1:2036790/71‑1 (MQ=255) | CAACCAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTC > W3110S.gb/478274‑478412 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |