| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 527,659 | A→G | 44.1% | K163E (AAA→GAA) | ylbH → | conserved hypothetical protein, rhs‑like |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 527,659 | 0 | A | G | 44.1% | 19.3 / 92.9 | 68 | K163E (AAA→GAA) | ylbH | conserved hypothetical protein, rhs‑like |
| Reads supporting (aligned to +/- strand): ref base A (18/20); new base G (12/18); total (30/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATTTTGTCGAGTGTCTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAAGTAAAGCTATCCCATTCTGA > W3110S.gb/527591‑527729 | cATTTTGTCGAGTGTCTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGaaa > 1:3356650/1‑69 (MQ=255) ttttGTCGAGTGTCTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAaga < 1:662144/71‑1 (MQ=255) ttGTCGAGTTTCTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGaaaaa > 1:3044023/1‑67 (MQ=255) ttGTCGAGTGTCTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGaaaaa > 1:2563174/1‑67 (MQ=255) gTCGAGTGTCTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAagagat < 1:4573586/70‑1 (MQ=255) tCGAGTGTCTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAagagatta < 1:2600026/71‑1 (MQ=255) gAGTGTCTAAATTAAATGACGCGGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAagagattag < 1:4047489/70‑1 (MQ=255) tgtCTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAagagattagagat < 1:1705352/71‑1 (MQ=255) gtCTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAagagattagagat < 1:2910445/70‑1 (MQ=255) tCTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATgaagaa < 1:139861/57‑1 (MQ=255) cTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAagagat > 1:3176522/1‑61 (MQ=255) taaattaaatGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGaaaa < 1:2753458/54‑1 (MQ=255) taaattaaatGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGaaaa < 1:153585/54‑1 (MQ=255) taaattaaatGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACg > 1:4299313/1‑71 (MQ=255) taaattaaatGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAaga > 1:2019308/1‑57 (MQ=255) aaattaaatGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAagagattagagat < 1:3687204/66‑1 (MQ=255) aaattaaatGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACgg > 1:1303465/1‑71 (MQ=255) aattaaatGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAagagatt > 1:1333741/1‑59 (MQ=255) attaaatGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACggg < 1:1613918/70‑1 (MQ=255) ttaaatGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGtt < 1:2870717/71‑1 (MQ=255) ttaaatGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGtt < 1:765991/71‑1 (MQ=255) taaatGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGtt > 1:3509620/1‑70 (MQ=255) aaatGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAagagat < 1:3995641/54‑1 (MQ=255) aaatGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAagagat < 1:1222311/54‑1 (MQ=255) aatGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAagagattagagat < 1:2336206/60‑1 (MQ=255) aatGACGCTGGTGTTAGCCGATCGGCGAAAGCGCTGGGTTATGAAAAagagattagagat < 1:4017849/60‑1 (MQ=255) tGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATc < 1:153163/71‑1 (MQ=255) aCGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTg < 1:2958293/71‑1 (MQ=255) cTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAagagatta < 1:1069880/48‑1 (MQ=255) cTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTc < 1:1758333/71‑1 (MQ=255) ggTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAagagatta > 1:889510/1‑46 (MQ=255) ggTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCgg > 1:251996/1‑71 (MQ=255) ggTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCgg > 1:645139/1‑71 (MQ=255) gtgtTAGCCGATCGGCGAAAGGGCTGGGTTGTGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGt < 1:2417360/71‑1 (MQ=255) gtgtTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCgg < 1:2899377/70‑1 (MQ=255) gtgtTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCgg < 1:2681247/70‑1 (MQ=255) gtgtTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCgg < 1:2218265/70‑1 (MQ=255) gtgtTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGt < 1:2361271/71‑1 (MQ=255) gtgtTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGt < 1:91338/71‑1 (MQ=255) tgtTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTAc < 1:2549583/52‑1 (MQ=255) tgtTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTAc < 1:2371067/52‑1 (MQ=255) gtTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAagaga > 1:3844852/1‑40 (MQ=255) ttAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAagaga > 1:3323775/1‑39 (MQ=255) ttAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATg < 1:2195597/71‑1 (MQ=255) ttAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATg < 1:1069319/71‑1 (MQ=255) aGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTa > 1:1928609/1‑71 (MQ=255) aGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAgagattagagatta > 1:5036124/1‑47 (MQ=255) gATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACgg > 1:5093410/1‑70 (MQ=255) aTCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTc < 1:2379490/58‑1 (MQ=255) aTCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTc < 1:4269330/58‑1 (MQ=255) ggCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGaa < 1:5064353/71‑1 (MQ=255) ggCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGaa < 1:3790071/71‑1 (MQ=255) cGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGaa > 1:4597164/1‑69 (MQ=255) cGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGaa > 1:2246566/1‑69 (MQ=255) cGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTa > 1:3845878/1‑57 (MQ=255) cGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTa > 1:3057752/1‑57 (MQ=255) cGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTa > 1:1374947/1‑57 (MQ=255) cGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACAGCAGaa > 1:194093/1‑69 (MQ=255) gAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAAg < 1:2794734/71‑1 (MQ=255) aaaTGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGaa < 1:2307882/67‑1 (MQ=255) aaaGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTaa < 1:4895447/43‑1 (MQ=255) aaaGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGaa < 1:1776698/67‑1 (MQ=255) aaaGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAAg > 1:2858342/1‑70 (MQ=255) aaaGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAAg > 1:1857536/1‑70 (MQ=255) aaaGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAAGt < 1:301419/71‑1 (MQ=255) aGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTa > 1:3571127/1‑57 (MQ=255) aGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAAGTa > 1:1300043/1‑70 (MQ=255) aGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAAGTa > 1:564187/1‑70 (MQ=255) ggCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAAGTAAAg > 1:5065179/1‑71 (MQ=255) gCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTa > 1:933951/1‑50 (MQ=255) gTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTgat > 1:1275532/1‑38 (MQ=38) gTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGtt > 1:3311406/1‑40 (MQ=255) gTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATg < 1:2509605/47‑1 (MQ=255) gAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTa > 1:3035721/1‑44 (MQ=255) aaaaaGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAAGTa > 1:3996342/1‑56 (MQ=255) aaaGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAAGTAAAGCTATCCCATTCTGa < 1:4884223/71‑1 (MQ=255) | CATTTTGTCGAGTGTCTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAAGTAAAGCTATCCCATTCTGA > W3110S.gb/527591‑527729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |