| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 530,077 | T→C | 15.4% | Y125C (TAT→TGT) | ybbB ← | tRNA 2‑selenouridine synthase, selenophosphate‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 530,077 | 0 | T | C | 15.4% | 126.2 / 16.7 | 52 | Y125C (TAT→TGT) | ybbB | tRNA 2‑selenouridine synthase, selenophosphate‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (25/19); new base C (5/3); total (30/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01 | |||||||||||
AATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTG > W3110S.gb/530010‑530147 | aaTCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCATGAATCGCGGTCTGGCGCAGTGCCTtat > 1:4360056/1‑68 (MQ=255) gCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCt < 1:4877436/71‑1 (MQ=255) gCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCt < 1:168656/71‑1 (MQ=255) cACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCtt > 1:1529282/1‑71 (MQ=255) tCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTccac > 1:4785151/1‑70 (MQ=255) ggTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCTCAGTGCCTTATAACCGCCTTCCACCAg > 1:821643/1‑71 (MQ=255) ggTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAg < 1:1485498/71‑1 (MQ=255) ggTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAg > 1:1245429/1‑71 (MQ=255) ccAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatca < 1:2769546/71‑1 (MQ=255) ccAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatca < 1:1708128/71‑1 (MQ=255) ccAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTACACCAGCGGATaatc < 1:2578044/70‑1 (MQ=255) aGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATaatcaat < 1:4340643/71‑1 (MQ=255) aGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATaatcaat < 1:4334781/71‑1 (MQ=255) taaaTAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCACCTTCCACCAGCGGATAATCAATccc > 1:369493/3‑71 (MQ=255) tCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATccc > 1:615316/1‑71 (MQ=255) cAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatcaa > 1:2125759/1‑66 (MQ=255) cAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatcaa > 1:4273866/1‑66 (MQ=255) cAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatcaa > 1:165282/1‑66 (MQ=255) cAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTAATAACCGCCTTCCACCAGCGGATAATCAATcccc > 1:4953116/1‑71 (MQ=255) cAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTAATAACCGCCTTCCACCAGCGGATAATCAATcccc > 1:2181231/1‑71 (MQ=255) cAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTAATAACCGCCTTCCACCAGCGGATAATCAATccc > 1:3086884/1‑70 (MQ=255) aaTAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCg < 1:3630935/71‑1 (MQ=255) tAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCg > 1:3200569/1‑69 (MQ=255) tAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCg > 1:2168653/1‑69 (MQ=255) aGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaa > 1:285343/1‑58 (MQ=255) aGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaa > 1:3773060/1‑58 (MQ=255) gCCTGAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATAATCAATcccc > 1:1442116/1‑63 (MQ=255) cTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatca < 1:4278371/55‑1 (MQ=255) cTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCtgcatgca < 1:2130751/71‑1 (MQ=255) cTGAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATAATCAATCCCCGCtgcatgca < 1:4604425/71‑1 (MQ=255) gAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGc > 1:2026557/1‑44 (MQ=255) gAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCg > 1:585776/1‑45 (MQ=255) gAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATaa > 1:5035042/1‑50 (MQ=255) gAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATaa > 1:248353/1‑50 (MQ=255) gAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATaa > 1:2709256/1‑50 (MQ=255) gAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAAc > 1:207819/1‑71 (MQ=255) aTCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatca < 1:1881911/51‑1 (MQ=255) aTCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatca < 1:360941/51‑1 (MQ=255) cgcgGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatcaat > 1:244613/1‑51 (MQ=255) cgcgGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatca > 1:2248325/1‑49 (MQ=255) cgcgGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatca > 1:331414/1‑49 (MQ=255) gcgcAGTGCCTTATAACCGCCTTCCACCAGCGGATaa > 1:347533/1‑37 (MQ=255) gcgcAGTGCCTTATAACCGCCTTCCACCAGCGGATaa > 1:5068301/1‑37 (MQ=255) gcgcAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGca < 1:2592699/68‑1 (MQ=255) gcgcAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGca < 1:4449566/68‑1 (MQ=255) cgcAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAat < 1:391659/71‑1 (MQ=255) tGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGcac < 1:2421970/63‑1 (MQ=255) tGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGcac < 1:4078586/63‑1 (MQ=255) tGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATAtg < 1:712633/69‑1 (MQ=255) tGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATAtg < 1:284474/69‑1 (MQ=255) gCCTTATAACCGCCTTCCACCAGCGGATAATCAATCACCGCTGCATGCAACCAGCttt > 1:383452/1‑58 (MQ=255) ccTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCtgca > 1:383236/1‑44 (MQ=255) ttATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAg < 1:4502296/51‑1 (MQ=255) ataACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATAtg > 1:1078189/1‑63 (MQ=255) ataACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATAtg > 1:375011/1‑63 (MQ=255) ataACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCt > 1:4652273/1‑71 (MQ=255) taACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCt > 1:3938337/1‑70 (MQ=255) taACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTg < 1:1024450/71‑1 (MQ=255) taACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTg < 1:935175/71‑1 (MQ=255) | AATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTG > W3110S.gb/530010‑530147 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |