| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 548,801 | G→A | 44.8% | pseudogene (1221/1259 nt) pseudogene (964/1002 nt) |
ylbE → ylbE → |
ECK0512:JW0508+JW0507:b4572; hypothetical protein ECK0512:JW0508:b0519; hypothetical protein, C‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 548,801 | 0 | G | A | 44.8% | 9.7 / 33.4 | 29 | pseudogene (1221/1259 nt) pseudogene (964/1002 nt) | ylbE ylbE | ECK0512:JW0508+JW0507:b4572; hypothetical protein ECK0512:JW0508:b0519; hypothetical protein, C‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base G (4/12); new base A (7/6); total (11/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGC > W3110S.gb/548731‑548871 | ccATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAgg < 1:876198/71‑1 (MQ=255) aTAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGgcg < 1:3947568/71‑1 (MQ=255) aTAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacg < 1:3895529/71‑1 (MQ=255) gCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAg > 1:1604894/1‑70 (MQ=255) gggCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGa < 1:243385/71‑1 (MQ=255) ggCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGaa < 1:1435538/71‑1 (MQ=255) cAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGaaac < 1:915597/71‑2 (MQ=255) aTTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTg < 1:2566445/51‑1 (MQ=255) aTTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAg < 1:1419765/68‑1 (MQ=255) aTTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAg < 1:4260394/68‑1 (MQ=255) tGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATgg > 1:3208188/1‑71 (MQ=255) tGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATgg > 1:1697893/1‑71 (MQ=255) gcgcAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATggg < 1:3749766/70‑1 (MQ=255) aGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTg < 1:4748486/71‑1 (MQ=255) aCCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTg < 1:124881/71‑1 (MQ=255) aCCGTGCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTg < 1:1621539/71‑1 (MQ=255) tGCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCAc > 1:4805358/1‑42 (MQ=255) cGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTAtt > 1:684346/1‑60 (MQ=255) cGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCt < 1:4914411/38‑1 (MQ=255) gCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAAt > 1:4158773/1‑70 (MQ=255) gCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAAt > 1:5107409/1‑70 (MQ=255) gCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATg < 1:3864354/71‑1 (MQ=255) gCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACg < 1:2880611/70‑1 (MQ=255) gCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAg < 1:5059147/38‑1 (MQ=255) cGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACg < 1:3595738/69‑1 (MQ=255) gTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGat < 1:4798166/70‑1 (MQ=255) gCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGatcat > 1:4156012/1‑71 (MQ=255) cTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGatcatc > 1:245869/1‑71 (MQ=255) tttGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTg < 1:2862277/47‑1 (MQ=255) tGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATcatcca < 1:4596927/70‑1 (MQ=255) gAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCaa > 1:4409772/1‑56 (MQ=255) gAACAGAAGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCaa > 1:2235269/1‑56 (MQ=255) gcgcTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGc < 1:292141/71‑1 (MQ=255) gcgcTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGc < 1:965851/71‑1 (MQ=255) | CCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGC > W3110S.gb/548731‑548871 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |