| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 593,201 | A→G | 22.2% | F265L (TTC→CTC) | cusS ← | sensory histidine kinase in two‑component regulatory system with CusR, senses copper ions |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 593,201 | 0 | A | G | 22.2% | 86.3 / 23.8 | 45 | F265L (TTC→CTC) | cusS | sensory histidine kinase in two‑component regulatory system with CusR, senses copper ions |
| Reads supporting (aligned to +/- strand): ref base A (17/18); new base G (2/8); total (19/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
GGGCGATTTCGGTTTGCGTTATGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTACCAGCTGTTC > W3110S.gb/593132‑593270 | gggCGATTTCGGTTTGCGTTATGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGaaa > 1:1172241/1‑71 (MQ=255)gggCGATTTCGGTTTGCGTTATGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGaaa < 1:4840279/71‑1 (MQ=255) ggCGATTTCGGTTTGCGTTATGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGaaa < 1:3302544/70‑1 (MQ=255) cGATTTCGGTTTGCGTTATGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTg > 1:2937057/1‑71 (MQ=255) taggTTTGCGTTATGAGAGTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTg > 1:2220526/3‑66 (MQ=255) tCGGTTTGCGTTATGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGa > 1:921457/1‑68 (MQ=255) gTTTGCGTTATGAGATTCGTATTTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTg < 1:4905979/63‑1 (MQ=255) cGTTATGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTaa < 1:2118947/71‑1 (MQ=255) gTTATGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTaaa < 1:4350132/71‑1 (MQ=255) tGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACa > 1:4808994/1‑71 (MQ=255) tGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACa > 1:2462418/1‑71 (MQ=255) aTTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTaaa < 1:1637617/63‑1 (MQ=255) aTTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGACATCCt > 1:1904261/1‑71 (MQ=255) ttCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGACATCCTc < 1:2487244/71‑1 (MQ=255) cGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCaa > 1:580878/1‑71 (MQ=255) gTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCaa < 1:4530761/70‑1 (MQ=255) gTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAAt < 1:1688735/71‑1 (MQ=255) tAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGACATCCTCa > 1:1914064/1‑68 (MQ=255) aTTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGAc > 1:3646026/1‑59 (MQ=255) aTTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGAc > 1:1774876/1‑59 (MQ=255) ttGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGACa < 1:4620077/59‑1 (MQ=255) ttGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGACATCCTc < 1:2786599/64‑1 (MQ=255) ttGGTGTGCGAATTTCGTGAGCGATATCCGCCGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGc < 1:2510380/71‑1 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGa > 1:3089083/1‑55 (MQ=255) gtgtGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTc < 1:403191/70‑1 (MQ=255) tgCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGACATCCTc < 1:1655904/58‑1 (MQ=255) tgCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTaaa < 1:4830326/49‑1 (MQ=255) tgCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGAt > 1:3147644/1‑70 (MQ=255) cGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGATATCCTCAATACGCTCGATCa < 1:1412720/70‑1 (MQ=255) cGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCa > 1:4845088/1‑70 (MQ=255) cGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCa < 1:1159027/70‑1 (MQ=255) cGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCa < 1:4071598/70‑1 (MQ=255) cGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCa > 1:2097239/1‑70 (MQ=255) tCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGtt > 1:2101414/1‑71 (MQ=255) tGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTa < 1:490356/36‑1 (MQ=255) tGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTa < 1:2257097/36‑1 (MQ=255) gAGCGATATCCGCTGAGAGATTGGACTGGCGGGTaaa < 1:975422/37‑1 (MQ=255) aGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAAc > 1:1627159/1‑70 (MQ=255) aGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAAc > 1:1633160/1‑70 (MQ=255) gCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGa < 1:4906642/71‑1 (MQ=255) gCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGa < 1:111522/71‑1 (MQ=255) gCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGa < 1:4346960/71‑1 (MQ=255) gATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCa < 1:780814/56‑1 (MQ=255) tCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGt < 1:3216528/69‑1 (MQ=255) tCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTAc < 1:4630513/71‑1 (MQ=255) ccGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTAc > 1:3942533/1‑70 (MQ=255) cGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTAc > 1:1331769/1‑69 (MQ=255) gaAATTTGACTGGCGTGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGa < 1:1242784/57‑1 (MQ=255) aaaTTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTACCAGCTGTTc > 1:756635/1‑71 (MQ=255) | GGGCGATTTCGGTTTGCGTTATGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTACCAGCTGTTC > W3110S.gb/593132‑593270 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |