| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 616,917 | A→G | 11.1% | N1180D (AAT→GAT) | entF → | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 616,917 | 0 | A | G | 11.1% | 117.9 / 7.0 | 45 | N1180D (AAT→GAT) | entF | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (26/14); new base G (2/3); total (28/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATTTCTTGGCTTGCTGGATACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAACCGCGAACGCGAGGCCTTCCTGGCAGCA > W3110S.gb/616855‑616979 | cATTTCTTGGCTTGCTGGATACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTg > 1:1034542/1‑71 (MQ=255) tCTTGGCTTGCTGGATACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTGATGGTCTGGAc < 1:4717139/70‑1 (MQ=255) tCTTGGCTTGCTGGATACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGAcc > 1:802059/1‑71 (MQ=255) tCTTGGCTTGCTGGATACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGAcc > 1:2374868/1‑71 (MQ=255) tCTTGGCTTGCTGGATACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGAcc > 1:4135023/1‑71 (MQ=255) cTTGGCTTGCTGGATACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGAccc > 1:1273500/1‑71 (MQ=255) ggCTTGCTGGATACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTGATGGTCTGGa > 1:1508508/1‑65 (MQ=255) ggCTTGCTGGATACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTGATGGTCTGGa > 1:4166315/1‑65 (MQ=255) ggCTTGCTGGATACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCgg > 1:1737138/1‑70 (MQ=255) gCTTGCTGGATACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGa < 1:1499805/70‑1 (MQ=255) gCTTGCTGGATACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGa < 1:3086789/70‑1 (MQ=255) cTGGATACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGc < 1:5003240/70‑1 (MQ=255) ggATACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTg > 1:2196907/1‑55 (MQ=255) gATACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTc > 1:63013/1‑52 (MQ=255) gATACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGc > 1:79578/1‑71 (MQ=255) aTACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCg < 1:185424/71‑1 (MQ=255) aTACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCg < 1:2866364/71‑1 (MQ=255) ccTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGga < 1:466939/70‑1 (MQ=255) cTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTa < 1:3344559/41‑1 (MQ=255) cgccAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAAc < 1:4465292/71‑1 (MQ=255) cAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAAc > 1:3019273/1‑68 (MQ=255) cAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAACcgc > 1:5042959/1‑71 (MQ=255) aGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCt > 1:1641689/1‑39 (MQ=255) aGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCt > 1:1635676/1‑39 (MQ=255) aGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCt > 1:894201/1‑39 (MQ=255) aGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAACcgc > 1:2528355/1‑70 (MQ=255) gAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAACcgcg > 1:4268022/1‑70 (MQ=255) aaCGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTaa > 1:2494173/1‑63 (MQ=255) aCGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAACCGCGAAcg > 1:5053190/1‑71 (MQ=255) aCGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAACCGCGAAcg > 1:4777176/1‑71 (MQ=255) aCGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAACCGCGAAcg > 1:1340249/1‑71 (MQ=255) cGCAAAACTGGCAGGAAAAAGAAGCTGATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAACCGCGAAcgc < 1:3826974/71‑1 (MQ=255) cGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAACCGCGAAcgc < 1:717018/71‑1 (MQ=255) gCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCgg < 1:898772/41‑1 (MQ=255) cAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGAtt > 1:4745334/1‑57 (MQ=255) cAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAACCGCGAACGCGa < 1:2951424/71‑1 (MQ=255) cAAAAATGGCAGGAAAAAGAAGCTAATGGTCTGGAcc > 1:2281053/1‑37 (MQ=255) aaaCTGGCAGCAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAACCGCGAAcgc < 1:121678/67‑1 (MQ=255) cAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAACCGCGAACGCGAGGCCTTCCt > 1:3660234/1‑71 (MQ=255) aGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGga < 1:2776062/43‑1 (MQ=255) ggAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGga < 1:4005228/42‑1 (MQ=255) gAAAAAGAAGCTGATGGTCTGGACCCGGAAGTGCTGGCGga < 1:214427/41‑1 (MQ=255) aaaaGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAACCGCGAACGCGAGGCCTTCCTGgcag > 1:16876/1‑71 (MQ=255) aaaaGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAACCGCGAACGCGAGGCCTTCCTGgcag > 1:120417/1‑71 (MQ=255) gaagCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAACCGCGAACGCGAGGCCTTCCTGgcagca > 1:2252394/1‑69 (MQ=255) cTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAACCGCGAAcgcg < 1:3657955/48‑1 (MQ=255) | CATTTCTTGGCTTGCTGGATACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAATGGTCTGGACCCGGAAGTGCTGGCGGAGATTAACCGCGAACGCGAGGCCTTCCTGGCAGCA > W3110S.gb/616855‑616979 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |