| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 639,164 | G→A | 36.5% | P63P (CCG→CCA) | ahpF → | alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 639,164 | 0 | G | A | 36.5% | 64.0 / 63.3 | 63 | P63P (CCG→CCA) | ahpF | alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (13/27); new base A (12/11); total (25/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GGCTGAAATCGCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCTCCCCGCTGGG > W3110S.gb/639095‑639226 | ggCTGAAATCGCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCg < 1:226317/70‑1 (MQ=255)ggCTGAAATCGCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCg < 1:327782/70‑1 (MQ=255) aaaTCGCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCtt < 1:4100763/69‑1 (MQ=255) aaaTCGCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCtt < 1:345181/69‑1 (MQ=255) aaTCGCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTcc > 1:4592191/1‑71 (MQ=255) aaTCGCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTcc > 1:2008697/1‑71 (MQ=255) tCGCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTg < 1:1369392/71‑1 (MQ=255) cAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATc < 1:1449240/71‑1 (MQ=255) cAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTGATc < 1:1096710/71‑1 (MQ=255) gAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTGATCAc > 1:4000960/1‑71 (MQ=255) cTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGAt < 1:4160202/65‑1 (MQ=255) cTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTGATc < 1:344292/66‑1 (MQ=255) tGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTcc < 1:4820691/60‑1 (MQ=255) tGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTGATc < 1:3159504/65‑1 (MQ=255) gTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAAcc > 1:1754604/1‑71 (MQ=255) tCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAAccc < 1:2948647/71‑1 (MQ=255) aGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCa < 1:2817748/70‑1 (MQ=255) aGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCa < 1:25896/70‑1 (MQ=255) aGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAg > 1:1098800/1‑71 (MQ=255) aGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTGAt < 1:424363/60‑1 (MQ=255) aCAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGt > 1:3625777/1‑71 (MQ=255) tCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAc < 1:3652551/71‑1 (MQ=255) aCCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAAcc < 1:598613/70‑1 (MQ=255) aCCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAAcc < 1:4986879/70‑1 (MQ=255) aCCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAAcc < 1:3509433/70‑1 (MQ=255) aCCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCa < 1:2498242/71‑1 (MQ=255) aCCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCa < 1:1398004/71‑1 (MQ=255) ccTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAg < 1:4062928/71‑1 (MQ=255) ccTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAg < 1:4747360/71‑1 (MQ=255) tttAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCttt < 1:2432496/41‑1 (MQ=255) tttAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCttt < 1:3789173/41‑1 (MQ=255) tttAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCtt < 1:854525/40‑1 (MQ=255) ttAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCa > 1:3419715/1‑67 (MQ=255) tAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTg < 1:4751409/43‑1 (MQ=255) aaaGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTGAt > 1:2200262/1‑44 (MQ=255) aaaGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTGAt > 1:313809/1‑44 (MQ=255) aaaGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTGAt > 1:4980615/1‑44 (MQ=255) aaaGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTGAt > 1:2396407/1‑44 (MQ=255) aagaagATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGt < 1:887209/56‑1 (MQ=255) aTAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTGATc < 1:2278854/38‑1 (MQ=255) tAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTg < 1:4677731/71‑1 (MQ=255) tAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTg < 1:159145/71‑1 (MQ=255) tAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTg < 1:1451022/71‑1 (MQ=255) aaCAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAgg > 1:1993215/1‑47 (MQ=255) aCAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGc < 1:712272/70‑1 (MQ=255) aGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAgg > 1:1772705/1‑71 (MQ=255) aGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAgg > 1:4459165/1‑71 (MQ=255) cTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAgg > 1:2672397/1‑53 (MQ=255) tGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAc > 1:4106694/1‑47 (MQ=255) gCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCTccc > 1:4637350/1‑71 (MQ=255) gTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCTCCCCGCt > 1:2501784/1‑71 (MQ=255) gTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCTCCCCGCt > 1:4937157/1‑71 (MQ=255) gTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCTCCCCGCt < 1:4858001/71‑1 (MQ=255) tGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCTCCCCGCTg < 1:5020408/71‑1 (MQ=255) tGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTg < 1:2164873/57‑1 (MQ=255) gCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCTCCCCGCTg > 1:253258/1‑70 (MQ=255) cGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCTCCCCGCTggg > 1:4538052/1‑71 (MQ=255) gTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAAcc < 1:3468170/39‑1 (MQ=255) gTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAAcc < 1:585486/39‑1 (MQ=255) tAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc < 1:2100154/38‑1 (MQ=255) tAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc > 1:2887507/1‑38 (MQ=255) tAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc < 1:522888/38‑1 (MQ=255) tAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc > 1:3838652/1‑38 (MQ=255) gCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAg > 1:1440633/1‑37 (MQ=255) gCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAg > 1:781042/1‑37 (MQ=255) gTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCTcccc < 1:2805820/57‑1 (MQ=255) | GGCTGAAATCGCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCTCCCCGCTGGG > W3110S.gb/639095‑639226 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |