| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 647,863 | A→G | 25.5% | M311T (ATG→ACG) | citF ← | citrate lyase, citrate‑ACP transferase (alpha) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 647,863 | 0 | A | G | 25.5% | 79.1 / 29.3 | 47 | M311T (ATG→ACG) | citF | citrate lyase, citrate‑ACP transferase (alpha) subunit |
| Reads supporting (aligned to +/- strand): ref base A (18/17); new base G (2/10); total (20/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGA > W3110S.gb/647795‑647932 | cagcaTGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtc < 1:3590723/71‑1 (MQ=255)cagcaTGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtc > 1:2320523/1‑71 (MQ=255) gcaTGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtcgt > 1:1655783/1‑71 (MQ=255) aTGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcgtcg > 1:4183566/1‑71 (MQ=255) aTGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtcgtcg > 1:1178802/1‑71 (MQ=255) tGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtcgtc > 1:3842208/1‑69 (MQ=255) tGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTcgc > 1:2739157/1‑71 (MQ=255) gCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTcgcg < 1:1094397/70‑1 (MQ=255) gCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTcgcg < 1:3822582/70‑1 (MQ=255) gCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTcgcg < 1:4311493/70‑1 (MQ=255) gCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCgg > 1:3839951/1‑71 (MQ=255) tCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTaa < 1:4456902/70‑1 (MQ=255) ctctGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGcc < 1:2180689/71‑1 (MQ=255) tGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCa < 1:1430540/69‑1 (MQ=255) gcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCaa > 1:2451247/1‑60 (MQ=255) gcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCaa > 1:1400574/1‑60 (MQ=255) tttGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt < 1:1604637/45‑1 (MQ=255) tttGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt < 1:1121511/45‑1 (MQ=255) tGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGcgcg < 1:4762047/70‑1 (MQ=255) tGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGcgcg < 1:3972511/70‑1 (MQ=255) tGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTaa > 1:4772470/1‑45 (MQ=255) tGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAAt > 1:1489057/1‑46 (MQ=255) tGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGcgcg > 1:2189854/1‑70 (MQ=255) tGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGcgcg > 1:1960387/1‑70 (MQ=255) tGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGcgcg > 1:2345680/1‑70 (MQ=255) tGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGcgcg > 1:1718966/1‑70 (MQ=255) gCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGcgcg < 1:2092024/69‑1 (MQ=255) cGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGaa < 1:4984017/70‑1 (MQ=255) gATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa < 1:4743626/40‑1 (MQ=255) gATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAatat < 1:3625094/71‑1 (MQ=255) gATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAatat < 1:2641309/71‑1 (MQ=255) cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATcgc > 1:3831733/1‑71 (MQ=255) aGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGt < 1:1410519/35‑1 (MQ=255) gACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAgg > 1:3099939/1‑47 (MQ=255) gACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAgg > 1:3435107/1‑47 (MQ=255) gACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAgg > 1:4718718/1‑47 (MQ=255) ttttCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGcgcg < 1:3335140/56‑1 (MQ=255) ttttCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCt < 1:3790173/68‑1 (MQ=255) tGCAGGTCAACCGTCGTCGCGGTAATACCGCCAAGGGCGAAGTCGgcgct < 1:3348175/50‑2 (MQ=255) gCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAata > 1:1575180/1‑53 (MQ=255) cAGGTCAACCGTCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAata < 1:3882266/52‑1 (MQ=255) ggTCAACCGTCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCt < 1:3938551/71‑1 (MQ=255) gTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATc < 1:1331977/51‑1 (MQ=255) tCAACCGTCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTc < 1:2730510/71‑1 (MQ=255) cATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGa < 1:2383577/71‑1 (MQ=255) cATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGa < 1:2550288/71‑1 (MQ=255) cATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGa < 1:3813398/71‑1 (MQ=255) aTCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTAc > 1:4528504/1‑52 (MQ=255) aTCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTAc > 1:3773932/1‑52 (MQ=255) | CAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGA > W3110S.gb/647795‑647932 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |