| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 695,215 | C→T | 31.8% | A55T (GCT→ACT) | miaB ← | isopentenyl‑adenosine A37 tRNA methylthiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 695,215 | 0 | C | T | 31.8% | 77.8 / 50.4 | 60 | A55T (GCT→ACT) | miaB | isopentenyl‑adenosine A37 tRNA methylthiolase |
| Reads supporting (aligned to +/- strand): ref base C (21/19); new base T (12/7); total (34/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01 | |||||||||||
AATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGAT > W3110S.gb/695147‑695283 | aaTCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCt > 1:3495205/1‑71 (MQ=255) aGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTtctc < 1:1767932/71‑1 (MQ=255) cTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTcgcg > 1:5046005/1‑70 (MQ=255) cTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTACTGAGCCTTCTcgcg > 1:3074331/1‑70 (MQ=255) ttctCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAg > 1:3346974/1‑69 (MQ=255) ttctCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAg > 1:156679/1‑69 (MQ=255) ttctCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAg > 1:555600/1‑69 (MQ=255) ttctCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAg > 1:2689640/1‑69 (MQ=255) ctctTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGc < 1:2773992/68‑1 (MQ=255) tttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGc < 1:3060633/64‑1 (MQ=255) tttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGc < 1:3641307/64‑1 (MQ=255) ttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGtt > 1:3576330/1‑70 (MQ=255) tAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGtt > 1:1162057/1‑69 (MQ=255) tAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGtt > 1:748868/1‑69 (MQ=255) aCAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTcagc < 1:1885621/71‑1 (MQ=255) aCAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagc < 1:2470147/71‑1 (MQ=255) gTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCgg < 1:3751540/50‑1 (MQ=255) gTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCgg < 1:2411861/50‑1 (MQ=255) tttCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTcag < 1:1902261/66‑1 (MQ=255) tttCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTcag < 1:4762280/66‑1 (MQ=255) tttCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAg < 1:2505378/55‑1 (MQ=255) tttCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAg < 1:2547945/55‑1 (MQ=255) tttCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAg < 1:1345750/55‑1 (MQ=255) tCCAGCGACCCAACGGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagcagcag > 1:3203795/1‑70 (MQ=255) ccAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAg < 1:4539874/52‑1 (MQ=255) cAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTCAgcagcagca < 1:1746275/70‑1 (MQ=255) gACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagcagcag < 1:4016702/64‑1 (MQ=255) gACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATc < 1:3094969/70‑1 (MQ=255) gACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATc < 1:2560249/70‑1 (MQ=255) cccAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGc < 1:499728/71‑1 (MQ=255) cAACTGATGGAAGACTTTTTCCTGAGACTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGctt > 1:3681733/1‑71 (MQ=255) aaCTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTcagcagca > 1:1251540/1‑58 (MQ=255) aaCTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTAcagcagc > 1:4310394/1‑57 (MQ=255) aCTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttct > 1:304058/1‑71 (MQ=255) aCTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttct > 1:789429/1‑71 (MQ=255) aCTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttct > 1:2258623/1‑71 (MQ=255) aCTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttct > 1:4019436/1‑71 (MQ=255) tGATGGAAGACTTTTTCCTGAGTCTTCTCGGGGATTGAGCAGGTGTTcagca > 1:2943248/1‑52 (MQ=255) tGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCttcttc > 1:4028872/1‑71 (MQ=255) gATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTcc > 1:1659082/1‑71 (MQ=255) tGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTCAGCATCAGCACATCCGcttct < 1:546750/66‑1 (MQ=255) ggAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGc > 1:975773/1‑70 (MQ=255) gAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCGCATCCGCTTCTTCCGcc > 1:519721/1‑70 (MQ=255) gAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGcc > 1:1136143/1‑70 (MQ=255) aaGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCAc > 1:2142581/1‑71 (MQ=255) aGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACg > 1:1179448/1‑71 (MQ=255) aCTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGc < 1:3036030/65‑1 (MQ=255) cTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCg < 1:1538660/71‑1 (MQ=255) tttttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTc < 1:5000465/37‑1 (MQ=255) tttCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGctt > 1:3964653/1‑54 (MQ=255) tttCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGctt > 1:4504632/1‑54 (MQ=255) tttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagcagca > 1:3612965/1‑42 (MQ=255) tttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATc > 1:4751169/1‑49 (MQ=255) tttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttct > 1:2165875/1‑56 (MQ=255) tttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGt > 1:2884032/1‑70 (MQ=255) tttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGt > 1:4314517/1‑70 (MQ=255) ttCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGt > 1:3928938/1‑69 (MQ=255) aGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTg < 1:2243115/69‑1 (MQ=255) aGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTg < 1:1296770/69‑1 (MQ=255) aGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGAt < 1:3395628/71‑1 (MQ=255) | AATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGAT > W3110S.gb/695147‑695283 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |