| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 701,092 | T→C | 23.2% | S309G (AGT→GGT) | nagC ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 701,092 | 0 | T | C | 23.2% | 106.7 / 31.1 | 56 | S309G (AGT→GGT) | nagC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (18/25); new base C (8/5); total (26/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGG > W3110S.gb/701028‑701159 | aTAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGc < 1:781491/70‑1 (MQ=255) aaTGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTg < 1:1156591/70‑1 (MQ=255) aTGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGtt < 1:1704189/71‑1 (MQ=255) ggCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACCATCGCCTTTGTTcg > 1:3944361/1‑71 (MQ=255) ggCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACCATCGCCTTTGTTcg > 1:2514798/1‑71 (MQ=255) gCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTcgc > 1:2807877/1‑71 (MQ=255) gCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACCATCGCCTTTGTTcgc > 1:2423768/1‑71 (MQ=255) gTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCACGGCttt > 1:4820426/1‑71 (MQ=255) ttttACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGc < 1:3624543/54‑1 (MQ=255) ttttACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGc < 1:4168942/54‑1 (MQ=255) tttACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTg > 1:820802/1‑70 (MQ=255) ttACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTAt > 1:4714532/1‑49 (MQ=255) tACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACCATCGCCTTTGTTCGCGGCTTTg > 1:950604/1‑68 (MQ=255) aTGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtt < 1:442230/70‑1 (MQ=255) aTGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtt < 1:1027698/70‑1 (MQ=255) tGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTg < 1:4236091/60‑1 (MQ=255) tGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtttt < 1:3790276/71‑1 (MQ=255) tGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtttt < 1:1984671/71‑1 (MQ=255) tGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtt < 1:3968552/69‑1 (MQ=255) gacgacCGACATACTCAATTACTTCCGACGCCAGACTAt > 1:4241902/1‑39 (MQ=255) acgacCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGa < 1:313560/71‑1 (MQ=255) cgacATACTCAATTACTTCCGACGCCAGACCATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTg < 1:930839/71‑1 (MQ=255) cgacATACTCAATTACTTCCGACCCCAGACCATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTg < 1:3344146/71‑1 (MQ=255) gacATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGttt > 1:3883404/1‑62 (MQ=255) gacATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGatt > 1:4298693/1‑62 (MQ=255) aTACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAgt > 1:1353268/1‑71 (MQ=255) tACTCAATTACTTCCGACGCCAGACTATCGCCTTTg < 1:3004720/36‑1 (MQ=255) tACTCAATTACTTCCGACGCCAGACTATCGCCTTTg < 1:3408494/36‑1 (MQ=255) tACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTg < 1:939602/60‑1 (MQ=255) tACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTg < 1:3030041/60‑1 (MQ=255) tACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTg < 1:3035881/60‑1 (MQ=255) aCTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTg < 1:554740/59‑1 (MQ=255) aaTTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAgtcgtc > 1:3926854/1‑69 (MQ=255) aTTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATa > 1:1471242/1‑48 (MQ=255) aTTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATa > 1:2186685/1‑48 (MQ=255) ttACTTCCGACGCCAGACCATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCa < 1:3626497/61‑1 (MQ=255) aCTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAg > 1:3461657/1‑42 (MQ=255) cTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCgg < 1:5028822/70‑1 (MQ=255) cTTCCGACGCCAGACCATCGCCTTTGTTCGCGGCtt > 1:322041/1‑36 (MQ=255) ccGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATgg > 1:2425149/1‑51 (MQ=255) ccGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATgg > 1:2033861/1‑51 (MQ=255) ccGACGCCAGACCATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGAt > 1:686151/1‑49 (MQ=255) ccGACGCCAGACCATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCgcc > 1:4173291/1‑66 (MQ=255) gCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGct > 1:2348517/1‑71 (MQ=255) ccAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtttt < 1:2094401/40‑1 (MQ=255) ccAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtttt < 1:1812914/40‑1 (MQ=255) ccAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtttt < 1:2801218/40‑1 (MQ=255) ccAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtttt < 1:2100298/40‑1 (MQ=255) ccAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGctc > 1:4736143/1‑71 (MQ=255) cAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGctct > 1:1426016/1‑71 (MQ=255) cAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGctc < 1:4352498/70‑1 (MQ=255) cAGACCATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGctct < 1:4683607/71‑1 (MQ=255) cAGACCATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGctct > 1:4961010/1‑71 (MQ=255) aGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTg < 1:179712/71‑1 (MQ=255) aGACCATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGa < 1:4254233/40‑1 (MQ=255) cTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTgg > 1:2448733/1‑69 (MQ=255) tATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAg < 1:500326/52‑1 (MQ=255) tATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAg < 1:814044/52‑1 (MQ=255) tATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAg < 1:2060412/52‑1 (MQ=255) | ATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGG > W3110S.gb/701028‑701159 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |