| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 702,273 | C→T | 27.5% | D301N (GAT→AAT) | nagA ← | N‑acetylglucosamine‑6‑phosphate deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 702,273 | 0 | C | T | 27.5% | 156.0 / 67.0 | 91 | D301N (GAT→AAT) | nagA | N‑acetylglucosamine‑6‑phosphate deacetylase |
| Reads supporting (aligned to +/- strand): ref base C (33/33); new base T (12/13); total (45/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
CAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCAC > W3110S.gb/702203‑702338 | cAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATc > 1:467061/1‑71 (MQ=255)cAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATc > 1:3164186/1‑71 (MQ=255) aTGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTca < 1:2451385/71‑1 (MQ=255) tGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCcac < 1:4517241/71‑1 (MQ=255) ttCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTcaca > 1:1451919/1‑70 (MQ=255) cAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTca > 1:263219/1‑62 (MQ=255) cAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTca > 1:2624606/1‑62 (MQ=255) cAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTc < 1:3042490/71‑1 (MQ=255) aGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTcc < 1:1178013/71‑1 (MQ=255) acgcacgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGt > 1:2888293/1‑68 (MQ=255) acgcacgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGt > 1:2261708/1‑68 (MQ=255) acgcacgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGt > 1:5046763/1‑68 (MQ=255) acgcacgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGt > 1:78012/1‑68 (MQ=255) cgcacgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTAc < 1:3282097/70‑1 (MQ=255) cacgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACgg > 1:4293696/1‑70 (MQ=255) cacgcCATCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACgg > 1:1190/1‑70 (MQ=255) ccTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgtagta < 1:1853072/71‑1 (MQ=255) ttCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTcacac < 1:3838360/50‑1 (MQ=255) tCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTc > 1:2735227/1‑55 (MQ=255) tCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGt < 1:1030050/58‑1 (MQ=255) tCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGt < 1:832659/58‑1 (MQ=255) tCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGt < 1:679024/58‑1 (MQ=255) tCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGt < 1:4678436/58‑1 (MQ=255) cAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTatat > 1:2266335/1‑70 (MQ=255) cAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATAtt > 1:2055841/1‑71 (MQ=255) cAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATAtt > 1:4567691/1‑71 (MQ=255) aTCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgtagta < 1:5144204/65‑1 (MQ=255) aTCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgtagta < 1:2072529/65‑1 (MQ=255) aTCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGt < 1:2143377/71‑1 (MQ=255) aTCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGt < 1:512002/71‑1 (MQ=255) tCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGtt > 1:1976993/1‑71 (MQ=255) cATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTa > 1:4661397/1‑55 (MQ=255) cATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTa > 1:4330493/1‑55 (MQ=255) cATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTa > 1:4410175/1‑55 (MQ=255) cATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTa > 1:2730550/1‑55 (MQ=255) cATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTa > 1:3248906/1‑55 (MQ=255) aTGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGtttt < 1:915027/71‑1 (MQ=255) aTGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGtttt < 1:3702922/71‑1 (MQ=255) aTGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGttt > 1:1740340/1‑70 (MQ=255) aTGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCca > 1:1158688/1‑40 (MQ=255) aTGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGttt > 1:971060/1‑70 (MQ=255) aTGGTTAAGGATGAACCGCTTAACGTAACGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGtttt < 1:4314427/71‑1 (MQ=255) tGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGtttt < 1:3721317/70‑1 (MQ=255) ggTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAg > 1:5062563/1‑45 (MQ=255) gTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTAc < 1:1962607/70‑1 (MQ=255) ttAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGtttt < 1:1320413/67‑1 (MQ=255) ttAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGtttt < 1:650559/67‑1 (MQ=255) ttAAGGATGAACCGCTTAACGTACCGTTCTCATCcacaca < 1:3030434/40‑1 (MQ=255) ttAAGGATGAACCGCTTAACGTACCGTTCTCATCcacaca < 1:4299235/40‑1 (MQ=255) aGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGt > 1:1771144/1‑41 (MQ=255) aTGAACCGCTTAACGTACCGTTCTGATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaa > 1:2139746/1‑70 (MQ=255) aTGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgta > 1:5061166/1‑49 (MQ=255) aTGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaa > 1:3280939/1‑70 (MQ=255) tGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGt < 1:4734470/41‑1 (MQ=255) gAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAAt > 1:4826338/1‑71 (MQ=255) gAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgtagta > 1:29018/1‑50 (MQ=255) gAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAAt > 1:4762020/1‑71 (MQ=255) gAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAAt > 1:4756012/1‑71 (MQ=255) aaCCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAAt > 1:2112833/1‑70 (MQ=255) aaCCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATg < 1:3471693/71‑1 (MQ=255) aaCCGCTTAACGTACCGTTCTCATCCACACAAAGTc < 1:3275478/36‑1 (MQ=255) aaCCGCTTAACGTACCGTTCTCATCCACACAAAGTc < 1:908900/36‑1 (MQ=255) aaCCGCTTAACGTACCGTTCTCATCCACACAAAGTc < 1:2159766/36‑1 (MQ=255) aaCCGCTTAACGTACCGTTCTCATCCACACAAAGTc < 1:1292100/36‑1 (MQ=255) aaCCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTg < 1:1591649/54‑1 (MQ=255) aaCCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATg < 1:3986043/71‑1 (MQ=255) ccGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaa < 1:3943497/65‑1 (MQ=255) ccGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaa < 1:4227976/65‑1 (MQ=255) ccGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaa < 1:807294/65‑1 (MQ=255) cGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAAc > 1:1332772/1‑71 (MQ=255) cGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAAc > 1:2891023/1‑71 (MQ=255) cGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAAc > 1:4122764/1‑71 (MQ=255) gCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACt > 1:2859804/1‑71 (MQ=255) cTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGgtag < 1:3804887/42‑1 (MQ=255) tAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaaaa > 1:4604941/1‑62 (MQ=255) tAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaaaa > 1:1859927/1‑62 (MQ=255) tAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaaaa > 1:3310257/1‑62 (MQ=255) tAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaaaa > 1:2514048/1‑62 (MQ=255) cGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCaa < 1:2320979/71‑1 (MQ=255) cGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCaa < 1:1205411/71‑1 (MQ=255) cGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCaa < 1:2191513/71‑1 (MQ=255) cGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCaa < 1:1827699/71‑1 (MQ=255) gTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAAt < 1:2752150/71‑1 (MQ=255) tACCGTTCTCATTCACACAAAGTCCGTTACGgtagt < 1:1836494/36‑1 (MQ=255) tACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATg > 1:4199659/1‑71 (MQ=255) aCCGTTCTCATCCACACAAAGTCCGTTACGgtagta < 1:1255393/36‑1 (MQ=255) gTTCTCATTCACACAAAGTCCGTTACGGTAGTATAtt > 1:547253/1‑37 (MQ=255) ctcATTCACACAAAGTCCGTTACGGTAGTATATTGtt > 1:1419816/1‑37 (MQ=255) ctcATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTc < 1:3609443/60‑1 (MQ=255) ctcATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCAc > 1:2763738/1‑71 (MQ=255) ctcATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCAc > 1:3561500/1‑71 (MQ=255) tcATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCaa < 1:4152756/61‑1 (MQ=255) tcATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCaa < 1:1118434/61‑1 (MQ=255) | CAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCAC > W3110S.gb/702203‑702338 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |