| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 707,707 | A→G | 25.9% | N398S (AAC→AGC) | glnS → | glutamyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 707,707 | 0 | A | G | 25.9% | 138.0 / 57.7 | 81 | N398S (AAC→AGC) | glnS | glutamyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (29/31); new base G (9/12); total (38/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01 | |||||||||||
ATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAA > W3110S.gb/707637‑707777 | aTGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTaa > 1:1845356/1‑71 (MQ=255) ggCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAAc > 1:1685040/1‑69 (MQ=255) cAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGc < 1:4374130/71‑1 (MQ=255) gTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTa > 1:3697402/1‑70 (MQ=255) tCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGt < 1:1020497/68‑1 (MQ=255) tCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGt < 1:3428612/68‑1 (MQ=255) tCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACa < 1:2627472/71‑1 (MQ=255) tCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACa < 1:2031645/71‑1 (MQ=255) aGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACaaa > 1:321596/1‑71 (MQ=255) aGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACaa > 1:3604632/1‑70 (MQ=255) gCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACaaa > 1:3715587/1‑67 (MQ=255) gCCGTTAAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTc > 1:660665/1‑71 (MQ=255) ccGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCt > 1:364920/1‑71 (MQ=255) ccGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCt > 1:226515/1‑71 (MQ=255) cGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTg > 1:392973/1‑71 (MQ=255) tttAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAg < 1:1620125/54‑1 (MQ=255) tttAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAg < 1:3556115/54‑1 (MQ=255) tttAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAg < 1:3458585/54‑1 (MQ=255) tttAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAg < 1:3254658/54‑1 (MQ=255) ttAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGt > 1:752368/1‑70 (MQ=255) ttAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTg > 1:1173338/1‑71 (MQ=255) ttAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTg > 1:3626650/1‑71 (MQ=255) ttAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTg > 1:1929327/1‑71 (MQ=255) aGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGAAGTACAAACGTCTGGTGc < 1:3877296/70‑1 (MQ=255) tGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACg < 1:3372980/57‑1 (MQ=255) gagaTTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTa > 1:2301056/1‑70 (MQ=255) agaTTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAg < 1:1621600/44‑1 (MQ=255) agaTTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAg < 1:4743078/44‑1 (MQ=255) agaTTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAg < 1:609098/44‑1 (MQ=255) agaTTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAg < 1:2089946/44‑1 (MQ=255) agaTTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTgg > 1:2353162/1‑60 (MQ=255) agaTTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACaaa > 1:4120075/1‑53 (MQ=255) agaTTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACaaa > 1:1407126/1‑53 (MQ=255) gaTTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaag < 1:2291013/71‑1 (MQ=255) aTTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAg < 1:4755183/45‑1 (MQ=255) tttGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAAc < 1:4745059/38‑1 (MQ=255) tttGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAAc < 1:3555068/38‑1 (MQ=255) ttGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTGGTGCTg > 1:4493862/1‑61 (MQ=255) ttGGATTGATCGCGCCGAGTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTGGTGCTGGGTAaagaag > 1:2202265/1‑71 (MQ=255) tGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaagaag > 1:3727299/1‑70 (MQ=255) tGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaagaag > 1:3932910/1‑70 (MQ=255) ggATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTa > 1:3703881/1‑43 (MQ=255) aTTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaag < 1:642083/64‑1 (MQ=255) aTTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaag < 1:446420/64‑1 (MQ=255) aTTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaag < 1:2275136/64‑1 (MQ=255) ttGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGc < 1:2213521/36‑1 (MQ=255) tGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaagaag > 1:3609350/1‑65 (MQ=255) tGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaagaa > 1:4288168/1‑64 (MQ=255) tGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaagaa > 1:678774/1‑64 (MQ=255) tGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaagaa > 1:4293967/1‑64 (MQ=255) tGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaag < 1:385620/62‑1 (MQ=255) gATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCt > 1:2747374/1‑71 (MQ=255) gATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGt < 1:140918/57‑1 (MQ=255) aTCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaagaa > 1:5083050/1‑62 (MQ=255) aTCGCGCCGATTTC‑‑CGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaagaa > 1:4410110/1‑60 (MQ=255) cgcgCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTGGTGCTGGGTAaagaa > 1:1632199/1‑60 (MQ=255) gcgcCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAAc < 1:3324994/39‑1 (MQ=255) gcgcCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCg > 1:410661/1‑70 (MQ=255) gcgaCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAAc < 1:128059/39‑1 (MQ=255) cgcCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTa < 1:159725/71‑1 (MQ=255) cgcCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTa < 1:4953329/71‑1 (MQ=255) gcCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTa < 1:153747/70‑1 (MQ=255) cGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaagaag > 1:464704/1‑56 (MQ=255) cGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATg > 1:5062101/1‑71 (MQ=255) gATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGc > 1:2413279/1‑71 (MQ=255) gATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGc > 1:1176186/1‑71 (MQ=255) gATTTCCGCGAAGAAGATAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGc > 1:3267300/1‑71 (MQ=255) aTTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAAt < 1:2518244/68‑1 (MQ=255) aTTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAAt < 1:3842677/68‑1 (MQ=255) aTTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAAt < 1:3325574/68‑1 (MQ=255) ttCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTaaa > 1:2234489/1‑48 (MQ=255) gaagaaGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGa > 1:125604/1‑70 (MQ=255) gaagaaGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGAt < 1:3004980/71‑1 (MQ=255) aagaagCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGAtt < 1:1848622/71‑1 (MQ=255) agaagCTAACAAGCTGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAAt < 1:1902348/58‑1 (MQ=255) gaagCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTaa < 1:2847531/71‑1 (MQ=255) agCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAgg < 1:5085969/71‑1 (MQ=255) gCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGc < 1:2327840/43‑1 (MQ=255) gCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTAtgt < 1:2200250/62‑1 (MQ=255) cTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaag < 1:3430576/36‑1 (MQ=255) cTAACAAGCAGTACAAACGTCTGGTGCTGGGTAaag < 1:943185/36‑1 (MQ=255) cTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTa < 1:809893/66‑1 (MQ=255) aaCAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAg > 1:1528763/1‑66 (MQ=255) aCAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTATGGCAGa < 1:657994/70‑1 (MQ=255) aCAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGaa > 1:3554467/1‑71 (MQ=255) aCAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGaa > 1:3235483/1‑71 (MQ=255) aCAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGa < 1:714796/70‑1 (MQ=255) | ATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAA > W3110S.gb/707637‑707777 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |