| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 711,161 | C→T | 34.4% | intergenic (‑93/+196) | fur ← / ← fldA | DNA‑binding transcriptional dual regulator/flavodoxin 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 711,161 | 0 | C | T | 34.4% | 70.2 / 57.8 | 61 | intergenic (‑93/+196) | fur/fldA | DNA‑binding transcriptional dual regulator/flavodoxin 1 |
| Reads supporting (aligned to +/- strand): ref base C (22/18); new base T (12/9); total (34/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
TCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTAC > W3110S.gb/711096‑711228 | tCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCt > 1:5063359/1‑70 (MQ=255) cACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTa > 1:51200/1‑70 (MQ=255) cACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTa < 1:51215/70‑1 (MQ=255) cACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTa > 1:1962920/1‑70 (MQ=255) cACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTa < 1:4704371/70‑1 (MQ=255) aCTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTa < 1:4010067/69‑1 (MQ=255) tCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATg > 1:157651/1‑70 (MQ=255) cATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGa > 1:1839581/1‑70 (MQ=255) cATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGa > 1:4744609/1‑70 (MQ=255) aTTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGaa < 1:3322045/70‑1 (MQ=255) aagaagTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACcac < 1:458537/71‑1 (MQ=255) gACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTccc > 1:3413063/1‑71 (MQ=255) aCAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGaa < 1:751039/57‑1 (MQ=255) aGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTgg > 1:4807022/1‑71 (MQ=255) gAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGc < 1:3407012/71‑1 (MQ=255) tttGCTCTTGAGATAATGCGTATCATTATTGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCaaa < 1:1523651/71‑1 (MQ=255) tttGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCaaa < 1:4798343/71‑1 (MQ=255) gCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAata < 1:4243357/70‑1 (MQ=255) ctctTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAatat < 1:4917902/70‑1 (MQ=255) tctTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCaa < 1:3125908/65‑1 (MQ=255) gagaTTATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGtt < 1:3676260/69‑1 (MQ=255) gagaTAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGtt < 1:2387781/69‑1 (MQ=255) gagaTAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGtt < 1:887239/69‑1 (MQ=255) gagaTAATGCGTATCATTATAGAATTGCCACGCCTaaa > 1:2811289/1‑38 (MQ=255) gagaTAATGCGTATCATTATAGAATTGCCACGCCTaaa > 1:919581/1‑38 (MQ=255) gagaTAATGCGTATCATTATAGAATTGCCACGCCTAAATGaa > 1:4179593/1‑42 (MQ=255) gagaTAATGCGTATCATTATAGAATTGCCACGCCTAAATGaa > 1:3165209/1‑42 (MQ=255) gagaTAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTtaa > 1:450881/1‑71 (MQ=255) gagaTAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTtaa > 1:696147/1‑71 (MQ=255) aaTGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaa > 1:2311117/1‑70 (MQ=255) aTGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTcc > 1:3586951/1‑48 (MQ=255) tGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCt < 1:292518/49‑1 (MQ=255) gCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACg > 1:405018/1‑71 (MQ=255) gCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACg > 1:2241232/1‑71 (MQ=255) cGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACaa > 1:5099495/1‑41 (MQ=255) cGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACaa > 1:1498760/1‑41 (MQ=255) aTCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTta < 1:1622300/58‑1 (MQ=255) tCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGt < 1:1254837/67‑1 (MQ=255) tCATTATAGAATTGCCACGCCTAAATGAAAACcac > 1:948626/1‑35 (MQ=255) tCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGt > 1:4327892/1‑39 (MQ=255) cATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAat < 1:563138/49‑1 (MQ=255) cATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaaaa > 1:359937/1‑63 (MQ=255) aTTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCaaa > 1:2511978/1‑47 (MQ=255) aTTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGca > 1:1683640/1‑69 (MQ=255) aTTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGca > 1:179467/1‑69 (MQ=255) aTTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAAc > 1:4874416/1‑63 (MQ=255) aTTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGca > 1:5014678/1‑69 (MQ=255) aTTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGca > 1:2512956/1‑69 (MQ=255) ttATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAg < 1:309803/71‑1 (MQ=255) ttATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAg < 1:1865755/71‑1 (MQ=255) taGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGtt > 1:2258952/1‑50 (MQ=255) taGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGtt > 1:4131212/1‑50 (MQ=255) taGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaa < 1:720905/56‑1 (MQ=255) taGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaa < 1:4396778/56‑1 (MQ=255) taGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTg > 1:3999268/1‑62 (MQ=255) taGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTg > 1:781581/1‑62 (MQ=255) gAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTa < 1:2035562/70‑1 (MQ=255) aaTTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAata < 1:4925735/42‑1 (MQ=255) gCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaaaa > 1:3112495/1‑51 (MQ=255) ccACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTAc < 1:5069528/71‑1 (MQ=255) ccACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTAc < 1:1976699/71‑1 (MQ=255) | TCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTAC > W3110S.gb/711096‑711228 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |