| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 713,886 | A→T | 8.3% | I160F (ATC→TTC) | seqA → | regulatory protein for replication initiation |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 713,886 | 0 | A | T | 8.3% | 369.2 / 18.0 | 130 | I160F (ATC→TTC) | seqA | regulatory protein for replication initiation |
| Reads supporting (aligned to +/- strand): ref base A (66/53); new base T (3/8); total (69/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 138 total aligned reads displayed.
GCCGAAACATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTGCGGAACTATCTAAAA > W3110S.gb/713816‑713956 | gCCGAAACATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGa < 1:1970520/71‑1 (MQ=255)gCCGAAACATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGa < 1:15019/71‑1 (MQ=255)gCCGAAACATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGa < 1:2637038/71‑1 (MQ=255) ccGAAACATGTGCCAGGCACGCCGTATTGGGTGATCACCAATACCAACACCGGCCGTAAATGCAGCATGAt > 1:1302320/1‑71 (MQ=255) ccGAAACATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGAt > 1:3068457/1‑71 (MQ=255) cGAAACATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATc < 1:5039507/71‑1 (MQ=255) gAAACATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATct < 1:4472542/71‑2 (MQ=255) gAAACATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCg < 1:1970306/71‑1 (MQ=255) gAAACATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCg < 1:4639039/71‑1 (MQ=255) gAAACATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCg < 1:584503/71‑1 (MQ=255) aaCATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGaa < 1:892533/71‑1 (MQ=255) aaCATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGaa < 1:4251314/71‑1 (MQ=255) aCATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCg < 1:3212738/68‑1 (MQ=255) aCATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGaa < 1:2369446/70‑1 (MQ=255) aCATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGaa > 1:2495775/1‑70 (MQ=255) aCATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGaa > 1:4019895/1‑70 (MQ=255) aCATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGaa > 1:2265712/1‑70 (MQ=255) aTGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCTGCCGTAAATGCAGCATGATCGAacac < 1:4874905/71‑1 (MQ=255) aTGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAGTGCAGCATGATCGAacac < 1:3020473/71‑1 (MQ=255) aTGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGaa > 1:4078959/1‑68 (MQ=255) tgtgCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAAcaca < 1:3581831/71‑1 (MQ=255) tgCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACAcatc < 1:1824388/71‑1 (MQ=255) ccAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACAcatcat < 1:4355968/71‑1 (MQ=255) ccAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACAcatcat > 1:4547735/1‑71 (MQ=255) cAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACACATCATg < 1:467779/71‑1 (MQ=255) cACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGTTCGAACACATCATGCAGt < 1:2157721/71‑1 (MQ=255) cACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGTTCGAACACATCATGCAGt < 1:3329531/71‑1 (MQ=255) cACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACACATCATGc > 1:247309/1‑68 (MQ=255) aCGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGTTCGAACACATCATGCAGTc < 1:3771358/71‑1 (MQ=255) aCGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGTTCGAACACATCATGCAGTc < 1:4089760/71‑1 (MQ=255) aCGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAaca > 1:4412572/1‑59 (MQ=255) gCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGa < 1:1366972/71‑1 (MQ=255) aTTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGc < 1:3639443/69‑1 (MQ=255) ttGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGTTCGAACACATCATGCAGTCGATGCAAt > 1:525486/1‑71 (MQ=255) ttGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGaa < 1:378857/49‑1 (MQ=255) ttGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGc < 1:2345800/68‑1 (MQ=255) ttGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAAt > 1:2638511/1‑71 (MQ=255) ttGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAAt > 1:2684655/1‑71 (MQ=255) ttGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAAt > 1:3005977/1‑71 (MQ=255) ttGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAAt > 1:3513616/1‑71 (MQ=255) ttGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAAt > 1:3874158/1‑71 (MQ=255) gggTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAAtt < 1:1190564/70‑1 (MQ=255) tGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCcg < 1:1912498/71‑1 (MQ=255) tGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCcg > 1:1352286/1‑71 (MQ=255) gATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGc < 1:3269735/62‑1 (MQ=255) gATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCcgg > 1:5050955/1‑71 (MQ=255) gATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCcgg > 1:819048/1‑71 (MQ=255) caccaacaccaaCACCGGCCGTAAATGCAGCATGATCGaa > 1:2550989/1‑40 (MQ=255) accaacaccaacACCGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCaa > 1:3545727/1‑60 (MQ=255) accaacaccaacACCGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCcggcgg < 1:1078362/70‑1 (MQ=255) aacaccaacaccGGCCGTAAATGCAGCATGATCGAACAcatc > 1:1280377/1‑42 (MQ=255) aacaccaacaccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTcc > 1:1253509/1‑61 (MQ=255) aacaccaacaccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAatt > 1:4271090/1‑71 (MQ=255) acaccaacaccGGCCGTAAATGCAGCATGATCGAACAcatca < 1:78247/42‑1 (MQ=255) acaccaacaccGGCCGTAAATGCAGCATGATCGAACAcatca < 1:2045793/42‑1 (MQ=255) acaccaacaccGGCCGTAAATGCAGCATGATCGAACACATCATg > 1:93591/1‑44 (MQ=255) acaccaacaccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGt > 1:937175/1‑48 (MQ=255) acaccaacaccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAatt > 1:1384024/1‑70 (MQ=255) acaccaacaccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAatt > 1:3886122/1‑70 (MQ=255) acaccaacaccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAatt > 1:693046/1‑70 (MQ=255) acaccaacaccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAatt > 1:3907538/1‑70 (MQ=255) acaccaacaccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAatt > 1:3166521/1‑70 (MQ=255) accaacaccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCaa > 1:1492762/1‑54 (MQ=255) accaacaccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCaa > 1:2067521/1‑54 (MQ=255) ccaacaccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAattgatt < 1:4889491/71‑1 (MQ=255) aacaccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAattgatt > 1:1191009/1‑69 (MQ=255) aacaccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAattgatt > 1:2891836/1‑69 (MQ=255) aacaccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAAttgattga < 1:2351695/71‑1 (MQ=255) acaccGGCCGTAAATGCAGCATGTTCGAACACATCATGc > 1:1965858/1‑39 (MQ=255) acaccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCcggcgg < 1:2282280/60‑1 (MQ=255) acaccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAattgatt > 1:743844/1‑68 (MQ=255) acaccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAattgatt > 1:2386721/1‑68 (MQ=255) acaacGGCCGTAAATGCAGCATGATCGAACACATCATg > 1:457894/1‑38 (MQ=255) caccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTgaga < 1:3032024/71‑1 (MQ=255) accGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTgag > 1:1280818/1‑69 (MQ=255) ccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGaa > 1:517065/1‑70 (MQ=255) ccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAg > 1:3638421/1‑71 (MQ=255) ccGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAg < 1:887880/71‑1 (MQ=255) cGTAAATGCAGCATGTTCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTg < 1:2799897/71‑1 (MQ=255) tAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAattgatt < 1:4657320/58‑1 (MQ=255) tAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTGCg > 1:2837875/1‑71 (MQ=255) aaTGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGaa > 1:1299656/1‑50 (MQ=255) aaTGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGaa > 1:3893930/1‑50 (MQ=255) aaTGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGaa > 1:3489511/1‑61 (MQ=255) aaTGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTGCgg > 1:2521121/1‑70 (MQ=255) aaTGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTGCgg > 1:3967046/1‑70 (MQ=255) aaTGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTGCgg > 1:3932866/1‑70 (MQ=255) aaTGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTGCgg < 1:1894445/70‑1 (MQ=255) aaTGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTGCGGa < 1:3810735/71‑1 (MQ=255) gcagcaTGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTGCGGAAc > 1:4035500/1‑70 (MQ=255) cagcaTGATCGAACACATCATGCAGTCGATGCAATTCCcggcgg < 1:5094773/44‑1 (MQ=255) cagcaTGATCGAACACATCATGCAGTCGATGCAATTCCcggcgg < 1:2686144/44‑1 (MQ=255) cagcaTGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTGCGGAACTa > 1:3843453/1‑71 (MQ=255) agcaTGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTGCGGAACTAt < 1:4057591/71‑1 (MQ=255) caTGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTGCTGATCTATc > 1:3337692/1‑70 (MQ=255) gATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTGCGGAACTATCTaa > 1:794229/1‑70 (MQ=255) aTCGAACACATCATGCAGTCGATGCAATTCCcggcgg < 1:2143112/37‑1 (MQ=255) aTCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTGCGGAACTATCTaaaa > 1:3061664/1‑71 (MQ=255) aTCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTGCGGAACTATCTaaaa > 1:3746914/1‑71 (MQ=255) aTCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTGCGGAACTATCTAAcg < 1:730402‑M1/71‑3 (MQ=255) | GCCGAAACATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATCGAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTGCGGAACTATCTAAAA > W3110S.gb/713816‑713956 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |