| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 740,630 | C→T | 42.1% | G234G (GGC→GGT) | phr → | deoxyribodipyrimidine photolyase, FAD‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 740,630 | 0 | C | T | 42.1% | 19.6 / 44.2 | 38 | G234G (GGC→GGT) | phr | deoxyribodipyrimidine photolyase, FAD‑binding |
| Reads supporting (aligned to +/- strand): ref base C (12/10); new base T (10/6); total (22/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTG > W3110S.gb/740563‑740696 | tACGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGTAc > 1:3841901/1‑70 (MQ=255)tACGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGTAc > 1:428216/1‑70 (MQ=255)tACGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGTAc > 1:2598443/1‑70 (MQ=255)tACGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGTAc > 1:4758029/1‑70 (MQ=255) cAGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCg < 1:4509037/71‑1 (MQ=255) ttttGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGttt < 1:4013196/71‑1 (MQ=255) ttGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGTACCAGCCGTTTGt > 1:3359475/1‑71 (MQ=255) ttGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGt > 1:3309815/1‑71 (MQ=255) ttGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGt > 1:2957972/1‑71 (MQ=255) gCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTc < 1:4736556/70‑1 (MQ=255) aCGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGTACCAGCCGTTTGTCGGCCAGc > 1:5072595/1‑71 (MQ=255) gCCGGAGAATATGGGCAACAACGAGATTTTCCGGCAGTGGAAGGTACCAGCCGTTTGt > 1:4689273/1‑58 (MQ=255) ccGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGTACCAGCCGTTTGTCGGCCAGCCTGGc < 1:4261695/70‑1 (MQ=255) ccGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGTACCAGCCGTTTGTCGGCCAGCCTGGc < 1:4531649/70‑1 (MQ=255) ccGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGTACCAGCCGTTTGTCGGCCAGCCTGGc < 1:1453174/70‑1 (MQ=255) gagaATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAAcg < 1:2999518/71‑1 (MQ=255) gaATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCGAGCCTGGCAAcggg > 1:791686/1‑71 (MQ=255) gaATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAAcggg > 1:1696473/1‑71 (MQ=255) atGAGCAACAACGAGATTTTCCGGCAGTGGAAGGTACCAGCCGTTTGTCGGCCAGCCTGGCAAcgggcggg > 1:4261243/1‑71 (MQ=255) tGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGt > 1:3121030/1‑71 (MQ=255) gCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCg < 1:2657093/39‑1 (MQ=255) gCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTAt < 1:2836494/71‑1 (MQ=255) aacaacGAGATTTTCCGGCAGTGGAAGGTACCAGc < 1:1317729/35‑1 (MQ=255) aacaacGAGATTTTCCGGCAGTGGAAGGTACCAGc < 1:5143275/35‑1 (MQ=255) aacaacGAGATTTTCCGGCAGTGGAAGGTACCAGc < 1:4748408/35‑1 (MQ=255) caacGAGATTTTCCGGCAGTGGAAGGTACCAGCCg > 1:4975850/1‑35 (MQ=255) acGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTAtcgcctc < 1:2783353/71‑1 (MQ=255) acGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTAtcgcctc < 1:2155655/71‑1 (MQ=255) cGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAAc > 1:2780836/1‑53 (MQ=255) gagaTTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTc > 1:4986845/1‑37 (MQ=255) gagaTTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTc > 1:4120061/1‑37 (MQ=255) gagaTTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTAtcgcctcgc < 1:3439398/71‑1 (MQ=255) gagaTTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAAAGGGCGGGTTAtcgcctcgc < 1:3420951/71‑1 (MQ=255) tCCGGCAGTGGAAGGTACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCt > 1:1358176/1‑71 (MQ=255) aGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATc > 1:4486047/1‑71 (MQ=255) gAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCtt > 1:1502119/1‑71 (MQ=255) gAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCt > 1:1917487/1‑70 (MQ=255) gAAGGCACCAGCCGTTTGT‑GGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCttg > 1:322466/1‑71 (MQ=255) | TACGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTG > W3110S.gb/740563‑740696 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |