Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 70,434 C→T 44.0% N16N (AAC→AAT araC → DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb70,4340CT44.0% 45.3 / 132.0 100N16N (AAC→AATaraCDNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base C (28/28);  new base T (20/24);  total (48/52)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

AATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGA  >  W3110S.gb/70366‑70500
                                                                    |                                                                  
aaTGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGc                                                                  >  1:1896894/1‑71 (MQ=255)
 aTGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGcc                                                                 <  1:267149/71‑1 (MQ=255)
    gtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCa                                                               >  1:3653782/1‑70 (MQ=255)
    gtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCAt                                                              >  1:2978590/1‑71 (MQ=255)
     tggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATc                                                             <  1:4577386/71‑1 (MQ=255)
     tggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATc                                                             <  1:4742893/71‑1 (MQ=255)
       ggAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCt                                                            <  1:245574/70‑1 (MQ=255)
        gAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCt                                                            <  1:1599176/69‑1 (MQ=255)
         aGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCtggt                                                         <  1:165158/71‑1 (MQ=255)
           tATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATc                                                             <  1:1808222/65‑1 (MQ=255)
           tATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATc                                                             <  1:3622166/65‑1 (MQ=255)
           tATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATc                                                             <  1:4705627/65‑1 (MQ=255)
           tATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCtggtgg                                                       <  1:3866708/71‑1 (MQ=255)
             tGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGc                                                                  >  1:3655875/1‑58 (MQ=255)
              gAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCgg                                                    >  1:1484473/1‑71 (MQ=255)
              gAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCgg                                                    >  1:4762053/1‑71 (MQ=255)
               aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCggg                                                   >  1:990544/1‑71 (MQ=255)
               aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCgg                                                    >  1:5015164/1‑70 (MQ=255)
               aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCgg                                                    <  1:2352845/70‑1 (MQ=255)
                aaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGt                                                  >  1:2758744/1‑71 (MQ=255)
                aaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGt                                                  >  1:3061842/1‑71 (MQ=255)
                aaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGt                                                  >  1:242943/1‑71 (MQ=255)
                aaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGt                                                  >  1:1802704/1‑71 (MQ=255)
                 aaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGtt                                                 >  1:2957367/1‑71 (MQ=255)
                  aGTATGGCTGAAGCGCAAAATGATCGCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                <  1:3657189/71‑1 (MQ=255)
                  aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                <  1:3432079/71‑1 (MQ=255)
                  aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGttt                                                <  1:3930543/71‑1 (MQ=255)
                  aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGttt                                                <  1:4609250/71‑1 (MQ=255)
                  aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGttt                                                <  1:507796/71‑1 (MQ=255)
                  aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGttt                                                <  1:3797085/71‑1 (MQ=255)
                   gTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGcc                                                                 >  1:2066716/1‑53 (MQ=255)
                    tATGGCTGAAGCCCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTaa                                              <  1:4966214/71‑1 (MQ=255)
                      tGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACg                                            >  1:288396/1‑71 (MQ=255)
                        gCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGcc                                          <  1:2816939/71‑1 (MQ=255)
                         cTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAAc                                             <  1:3721467/67‑1 (MQ=255)
                         cTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCg                                         <  1:551924/71‑1 (MQ=255)
                         cTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCg                                         <  1:1497867/71‑1 (MQ=255)
                         cTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGcc                                          >  1:394238/1‑70 (MQ=255)
                         cTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCg                                         <  1:1373708/71‑1 (MQ=255)
                         cTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCg                                         <  1:3298338/71‑1 (MQ=255)
                           gAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGc                                           >  1:4625723/1‑67 (MQ=255)
                              gcgcAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCt                                                            <  1:3646541/47‑1 (MQ=255)
                                  aaaaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGc                                 >  1:338719/1‑70 (MQ=255)
                                   aaaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCa                               <  1:2561075/71‑1 (MQ=255)
                                   aaaTGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGttt                                                <  1:3136608/54‑1 (MQ=255)
                                    aaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCaa                              >  1:2361240/1‑71 (MQ=255)
                                      tGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACg                            >  1:907796/1‑71 (MQ=255)
                                        aTCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTg                                     <  1:4444437/60‑1 (MQ=255)
                                        aTCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACgg                           <  1:5068319/70‑1 (MQ=255)
                                        aTCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACgg                           <  1:3487323/70‑1 (MQ=255)
                                        aTCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGt                          <  1:2806212/71‑1 (MQ=255)
                                        aTCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGt                          <  1:1843808/71‑1 (MQ=255)
                                         tCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGttt                                                <  1:3206070/48‑1 (MQ=255)
                                          ccccTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                          <  1:2901112/53‑1 (MQ=255)
                                           cccTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTaa                                              >  1:3147772/1‑48 (MQ=255)
                                           cccTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTaa                                              >  1:3837681/1‑48 (MQ=255)
                                           cccTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTaa                                              >  1:196376/1‑48 (MQ=255)
                                           cccTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGtt                         >  1:1310007/1‑69 (MQ=255)
                                             ctgctgCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                <  1:106714/44‑1 (MQ=255)
                                             ctgctgCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGtt                                                 <  1:143703/43‑1 (MQ=255)
                                             ctgctgCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGa                                        >  1:2096584/1‑52 (MQ=255)
                                               gctgcCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTaa                                              >  1:4200238/1‑44 (MQ=255)
                                                ctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                          <  1:4677659/47‑1 (MQ=255)
                                                ctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                          <  1:3427090/47‑1 (MQ=255)
                                                ctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                          <  1:3061983/47‑1 (MQ=255)
                                                ctgcCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTaa                                              >  1:5116976/1‑43 (MQ=255)
                                                ctgcCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTaa                                              >  1:1444504/1‑43 (MQ=255)
                                                ctgcCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGa                  >  1:406946/1‑71 (MQ=255)
                                                      ggATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCTAtttttt            <  1:4648166/71‑1 (MQ=255)
                                                      ggATACTCGTTTAACGCCCATCTCGTGGCGGGTTTAACGCCGATTGAGGCCTACGGTTATCTCGAtttttt            <  1:4692152/71‑1 (MQ=255)
                                                       gATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttttt           <  1:217212/71‑1 (MQ=255)
                                                        aTACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTa          <  1:292068/71‑1 (MQ=255)
                                                        aTACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTa          <  1:298182/71‑1 (MQ=255)
                                                        aTACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTa          >  1:3225963/1‑71 (MQ=255)
                                                         tACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                          <  1:644454/38‑1 (MQ=255)
                                                         tACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                          <  1:2019560/38‑1 (MQ=255)
                                                         tACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAgg                                  <  1:2452674/46‑1 (MQ=255)
                                                           cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGcc                                >  1:1250320/1‑46 (MQ=255)
                                                           cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGcc                                >  1:3411486/1‑46 (MQ=255)
                                                           cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGcc                                >  1:236433/1‑46 (MQ=255)
                                                           cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtt                >  1:3700585/1‑62 (MQ=255)
                                                           cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc        <  1:3767239/70‑1 (MQ=255)
                                                           cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc        <  1:3520597/70‑1 (MQ=255)
                                                           cTCGTTTAATGCCCATCTGGTGGCGGGGTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc        <  1:3378775/70‑1 (MQ=255)
                                                           cTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAtctc                    >  1:4185421/1‑58 (MQ=255)
                                                           cTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAtctc                    >  1:4642621/1‑58 (MQ=255)
                                                           cTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAtctc                    >  1:2557307/1‑58 (MQ=255)
                                                           cTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc        <  1:3235088/70‑1 (MQ=255)
                                                             cGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGa                                    >  1:477013/1‑40 (MQ=255)
                                                             cGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgac     >  1:626156/1‑71 (MQ=255)
                                                              gTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgacc    >  1:2912040/1‑71 (MQ=255)
                                                              gTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc        >  1:2812591/1‑67 (MQ=255)
                                                              gTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgacc    >  1:3364917/1‑71 (MQ=255)
                                                              gTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgac     <  1:2091340/70‑1 (MQ=255)
                                                                ttAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGACAACGGTTATCTCGATTTTTTTATcgaccga  >  1:633962/1‑71 (MQ=255)
                                                                taaaCGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCaa                              >  1:3360011/3‑43 (MQ=255)
                                                                 tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGtt                         >  1:4920195/1‑47 (MQ=255)
                                                                 tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAt                       >  1:427724/1‑49 (MQ=255)
                                                                 tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtt                >  1:3171453/1‑56 (MQ=255)
                                                                 tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtt                >  1:1877499/1‑56 (MQ=255)
                                                                    |                                                                  
AATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGA  >  W3110S.gb/70366‑70500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: