| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 833,307 | G→T | 45.3% | T22T (ACC→ACA) | ybiA ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 833,307 | 0 | G | T | 45.3% | 61.2 / 67.2 | 64 | T22T (ACC→ACA) | ybiA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (21/14); new base T (10/19); total (31/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01 | |||||||||||
CAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTA > W3110S.gb/833242‑833374 | cAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGc > 1:2892772/1‑70 (MQ=255)cAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCt < 1:100515/71‑1 (MQ=255) aGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTg > 1:385840/1‑71 (MQ=255) ttttCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAg < 1:1565913/71‑1 (MQ=255) tttCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAg > 1:3258263/1‑70 (MQ=255) tttCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAg > 1:597304/1‑70 (MQ=255) tttCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTa > 1:3587999/1‑69 (MQ=255) tttCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAg > 1:1696896/1‑70 (MQ=255) tttCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAg > 1:1151857/1‑70 (MQ=255) ttCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGa < 1:2509516/70‑1 (MQ=255) cAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTat > 1:4148988/1‑70 (MQ=255) cAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTat > 1:2902083/1‑70 (MQ=255) gATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTatgatg < 1:1619366/67‑1 (MQ=255) tGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAAtt < 1:1489738/58‑1 (MQ=255) ggCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCtt < 1:4742878/70‑1 (MQ=255) ggCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTg < 1:2252050/71‑1 (MQ=255) ccAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTa > 1:1308134/1‑56 (MQ=255) ccAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTa > 1:779301/1‑56 (MQ=255) cAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCAt < 1:3881196/71‑1 (MQ=255) aGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCAt < 1:3012667/70‑1 (MQ=255) ggcggcAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCAt < 1:3287212/69‑1 (MQ=255) gcggcAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTg < 1:490002/42‑1 (MQ=255) gcggcAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATgg > 1:3231161/1‑58 (MQ=255) cggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGgt > 1:3993063/1‑58 (MQ=255) cggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGgt > 1:11637/1‑58 (MQ=255) ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTa > 1:262971/1‑42 (MQ=255) ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACgt > 1:3246833/1‑71 (MQ=255) ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACgt < 1:1515470/71‑1 (MQ=255) gcAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACgtg < 1:168092/71‑1 (MQ=255) aaaGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGtt > 1:5102079/1‑71 (MQ=255) aaGTTAGAAAAATCACCGTAGTCATCGCTTGTGCt < 1:5083246/35‑1 (MQ=255) aaGTTAGAAAAATCACCGTAGTCATCGCTTGTGCt < 1:762709/35‑1 (MQ=255) aaGTTAGAAAAATCACCGTAGTCATCGCTTGTGCt < 1:1089189/35‑1 (MQ=255) aaGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGgtgt < 1:3255989/55‑1 (MQ=255) aGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTgg < 1:1519817/71‑1 (MQ=255) aGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTg > 1:4947780/1‑66 (MQ=255) aaaaaTCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCtt < 1:2655965/71‑1 (MQ=255) aaaaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCtt < 1:952870/70‑1 (MQ=255) aaaTCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGAc > 1:2209352/1‑56 (MQ=255) aaaTCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTtg > 1:2722293/1‑71 (MQ=255) aaaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTatgatg > 1:3111059/1‑42 (MQ=255) aaaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTatgatg > 1:1393436/1‑42 (MQ=255) tCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGc < 1:1085560/71‑1 (MQ=255) ccGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCg < 1:4371348/71‑1 (MQ=255) ccGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCg < 1:2937896/71‑1 (MQ=255) cGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGAT‑CTTTTTGCTCGaa < 1:2327721/71‑1 (MQ=255) tCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACggg < 1:431062/71‑1 (MQ=255) tCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACggg < 1:2086901/71‑1 (MQ=255) tCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACggg < 1:4393984/71‑1 (MQ=255) cATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACggg > 1:1051818/1‑70 (MQ=255) aTCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCg > 1:4257934/1‑63 (MQ=255) tCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCt < 1:1627606/53‑1 (MQ=255) tCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCt < 1:597370/53‑1 (MQ=255) tCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCt < 1:4644321/53‑1 (MQ=255) tCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCt < 1:3484874/53‑1 (MQ=255) gCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTa < 1:998747/71‑1 (MQ=255) gCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGa > 1:4071639/1‑38 (MQ=255) gCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGa > 1:3026077/1‑38 (MQ=255) gCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGa > 1:1705183/1‑38 (MQ=255) gCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTgg > 1:3617190/1‑46 (MQ=255) gCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCttt > 1:4596131/1‑53 (MQ=255) gCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCttt > 1:853309/1‑53 (MQ=255) gCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCttt > 1:1025342/1‑53 (MQ=255) gCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTa < 1:3388190/71‑1 (MQ=255) | CAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTA > W3110S.gb/833242‑833374 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |