| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 837,886 | C→T | 16.3% | intergenic (+28/+201) | ybiC → / ← ybiJ | predicted dehydrogenase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 837,886 | 0 | C | T | 16.3% | 125.6 / 14.5 | 49 | intergenic (+28/+201) | ybiC/ybiJ | predicted dehydrogenase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (30/11); new base T (4/4); total (34/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATA > W3110S.gb/837822‑837955 | gaCGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAAc > 1:612162/1‑69 (MQ=255)gaCGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAAc > 1:4167548/1‑69 (MQ=255) tGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGt > 1:1744236/1‑70 (MQ=255) gCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGTAAACAAGGGTa < 1:1573537/70‑1 (MQ=255) gCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACaa > 1:1019271/1‑65 (MQ=255) gCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTaa > 1:3458995/1‑71 (MQ=255) tttCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAg > 1:3114758/1‑70 (MQ=255) tttCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAg > 1:3023104/1‑70 (MQ=255) tttCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAg > 1:2727167/1‑70 (MQ=255) tCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCCTCCAGTGGCGGACGGGCAAACAAGGGTAACATAg > 1:2822815/1‑68 (MQ=255) tCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAAt < 1:1578657/71‑1 (MQ=255) agcagTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCaa > 1:2317086/1‑68 (MQ=255) agcagTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCaa > 1:303236/1‑68 (MQ=255) tAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGTAAACAAGGGTAAc > 1:1329851/1‑52 (MQ=255) tAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGTAAACAAGGGTAAc > 1:286649/1‑52 (MQ=255) aGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTa > 1:493667/1‑66 (MQ=255) aGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCa < 1:2597459/71‑1 (MQ=255) aGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCa < 1:2740251/71‑1 (MQ=255) tAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCatat > 1:4565282/1‑71 (MQ=255) tAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCatat > 1:4196016/1‑71 (MQ=255) tAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCatat > 1:2350289/1‑71 (MQ=255) aaaaaaaaGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAg > 1:1226192/1‑47 (MQ=255) aaaaaaGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGc < 1:163926/70‑1 (MQ=255) aaaaGCCCGTCCAGTGGCGGACGGGTAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTc > 1:923655/1‑71 (MQ=255) ccGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATTAATGAGGGTTAGAGCATATGCGTCTGTCg > 1:3590476/1‑70 (MQ=255) ccGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCgg > 1:2952932/1‑71 (MQ=255) ccGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCg < 1:1251947/70‑1 (MQ=255) cGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCgg > 1:5071362/1‑70 (MQ=255) tCCTGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATg < 1:2237887/42‑1 (MQ=255) tCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATc < 1:422040/38‑1 (MQ=255) cAGTGGCGGACGGGTAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCaaa < 1:2089007/70‑1 (MQ=255) cAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCaa > 1:248682/1‑38 (MQ=255) cAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCaa > 1:4629388/1‑38 (MQ=255) cAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCaaa > 1:1954722/1‑70 (MQ=255) cAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCaaa > 1:4502814/1‑70 (MQ=255) cAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCaaa > 1:3405760/1‑70 (MQ=255) gTGGCGGACGGGTAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGGGTCTGTCGGCa < 1:4307048/66‑1 (MQ=255) gTGGCGGACGGGTAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCa < 1:203883/66‑1 (MQ=255) gTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCaaa > 1:4588965/1‑68 (MQ=255) gTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCaaa > 1:3497274/1‑68 (MQ=255) ggCGGACGGGTAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAacaga > 1:4376336/1‑70 (MQ=255) ggCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCata > 1:915972/1‑50 (MQ=255) gCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAacagaca < 1:3508152/71‑1 (MQ=255) gACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCaaa < 1:3618264/62‑1 (MQ=255) gggCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGaaa < 1:2375083/71‑1 (MQ=255) ggCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCaaa > 1:567121/1‑58 (MQ=255) gCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATa > 1:3540611/1‑71 (MQ=255) gCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATa > 1:3555509/1‑71 (MQ=255) gCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATa < 1:3642086/71‑1 (MQ=255) cAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAac > 1:1517717/1‑57 (MQ=255) | GACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATA > W3110S.gb/837822‑837955 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |