| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 861,111 | G→A | 35.1% | L640L (CTG→TTG) | ybiW ← | predicted pyruvate formate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 861,111 | 0 | G | A | 35.1% | 63.4 / 54.7 | 57 | L640L (CTG→TTG) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base G (13/24); new base A (14/6); total (27/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.46e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01 | |||||||||||
TGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTA > W3110S.gb/861043‑861177 | tgaCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGtt < 1:2696483/71‑1 (MQ=255)tgaCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGtt < 1:1432913/71‑1 (MQ=255) aCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcg > 1:4147928/1‑71 (MQ=255) gTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgtc > 1:3523417/1‑71 (MQ=255) tACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgtcg < 1:1282356/71‑1 (MQ=255) aCCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgtcg < 1:2441284/70‑1 (MQ=255) cccGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:4925954/70‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGatat < 1:2144494/71‑1 (MQ=255) cgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAg < 1:1374212/71‑1 (MQ=255) tAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGt < 1:3599830/57‑1 (MQ=255) tAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:3861435/64‑1 (MQ=255) tAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGt > 1:2997631/1‑71 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt > 1:4951682/1‑71 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCt > 1:3885783/1‑71 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCt > 1:2557563/1‑71 (MQ=255) tAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAg < 1:1831219/66‑1 (MQ=255) tAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAg < 1:4479774/66‑1 (MQ=255) gTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGt > 1:4865213/1‑66 (MQ=255) gTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGa > 1:2580554/1‑70 (MQ=255) tGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATa < 1:865831/70‑1 (MQ=255) gccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAgcgc < 1:199855/71‑1 (MQ=255) aaCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGa > 1:4862934/1‑70 (MQ=255) aaCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAg > 1:1607168/1‑71 (MQ=255) ccGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt < 1:4380620/57‑1 (MQ=255) cGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGAtata > 1:2966023/1‑51 (MQ=255) cGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGcc < 1:5145339/70‑1 (MQ=255) cGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATaa > 1:1348817/1‑61 (MQ=255) cGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATaa > 1:1051583/1‑61 (MQ=255) ggACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCcag > 1:4038961/1‑71 (MQ=255) ccACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCcagcag < 1:2592458/71‑1 (MQ=255) ccACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCcagcag > 1:2838576/1‑71 (MQ=255) cACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCcagcag < 1:875159/70‑1 (MQ=255) aCGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcgc < 1:306357/59‑1 (MQ=255) aCGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg > 1:2031801/1‑71 (MQ=255) cGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGAtata > 1:2932438/1‑44 (MQ=255) cGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg > 1:1969905/1‑70 (MQ=255) cGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGt < 1:2613734/71‑1 (MQ=255) cGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAg > 1:1397353/1‑41 (MQ=255) gTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCATCAGCGTATCGa < 1:3127468/71‑1 (MQ=255) gTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGa < 1:2414963/71‑1 (MQ=255) gTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGa < 1:4857184/71‑1 (MQ=255) cgcgGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGt < 1:3470682/71‑1 (MQ=255) cgcgGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGt < 1:2809458/71‑1 (MQ=255) gATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcgc < 1:2596604/45‑1 (MQ=255) gATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcgc < 1:2280321/45‑1 (MQ=255) gATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcgc < 1:4970165/45‑1 (MQ=255) aTTATGGTACTGTTTCAGTTCGTCGATATAGGTCt < 1:214345/35‑1 (MQ=255) aTTATGGTACTGTTTCAGTTCGTCGATATAGGTCt < 1:1811852/35‑1 (MQ=255) tATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCAtcg > 1:3356060/1‑71 (MQ=255) tGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCAtcgtc < 1:645603/71‑1 (MQ=255) gTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATcgtcgt > 1:4561837/1‑71 (MQ=255) gTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATcgtcgt > 1:3678870/1‑71 (MQ=255) gTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGAGAGCCAGCAGCGTATCGACAGTATCATcgtcgt > 1:1457864/1‑71 (MQ=255) aCTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTg > 1:4255439/1‑71 (MQ=255) gTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCg > 1:4331342/1‑71 (MQ=255) gTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCg > 1:2636280/1‑71 (MQ=255) ttCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTa > 1:2102297/1‑71 (MQ=255) | TGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTA > W3110S.gb/861043‑861177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |