| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 862,250 | G→T | 27.9% | T260N (ACT→AAT) | ybiW ← | predicted pyruvate formate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 862,250 | 0 | G | T | 27.9% | 89.5 / 16.2 | 39 | T260N (ACT→AAT) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base G (16/12); new base T (3/8); total (19/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACG > W3110S.gb/862181‑862314 | gAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGt > 1:4180503/1‑71 (MQ=255)gAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGt > 1:2939907/1‑71 (MQ=255) aCCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTgcg > 1:2928988/1‑71 (MQ=255) cGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTc > 1:3002618/1‑65 (MQ=255) aGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCt > 1:4739060/1‑71 (MQ=255) aGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCt > 1:2757309/1‑71 (MQ=255) aGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCt > 1:894781/1‑71 (MQ=255) ttCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCt < 1:3819038/68‑1 (MQ=255) ttCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCt < 1:4917184/68‑1 (MQ=255) ttCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCt < 1:3825116/68‑1 (MQ=255) tCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCtggtg > 1:2371693/1‑71 (MQ=255) cGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCtggtgg > 1:2566181/1‑71 (MQ=255) tGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCt < 1:3361222/61‑1 (MQ=255) tGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTTGTGGGCg < 1:2079925/69‑1 (MQ=255) cAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCtgg > 1:1414587/1‑61 (MQ=255) cAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCtgg > 1:1681076/1‑61 (MQ=255) cAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGAt < 1:1069367/69‑1 (MQ=255) cAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGAt < 1:1075346/69‑1 (MQ=255) aaaaTCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCTGGTGGGc < 1:5083984/65‑1 (MQ=255) aaaTCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGAt > 1:1555455/1‑67 (MQ=255) aaTCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCTGGTGGGCGATAAGAt < 1:3959581/71‑1 (MQ=255) cTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGt < 1:4767379/71‑1 (MQ=255) gATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGtttt < 1:4221449/71‑1 (MQ=255) gATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGtttt < 1:1176643/71‑1 (MQ=255) aTGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTc < 1:1777543/71‑1 (MQ=255) aGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGc > 1:2630387/1‑70 (MQ=255) gTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGAt < 1:3079561/51‑1 (MQ=255) aaCACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCtg > 1:2988339/1‑40 (MQ=255) acacAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATc < 1:4919790/70‑1 (MQ=255) tGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGAt > 1:4182726/1‑46 (MQ=255) tGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGAt > 1:3331755/1‑46 (MQ=255) tGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGAt > 1:896120/1‑46 (MQ=255) gCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCcagcatt > 1:4456941/1‑69 (MQ=255) cAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGtt < 1:944138/71‑1 (MQ=255) gcCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCa > 1:3127963/1‑44 (MQ=255) gCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGtt < 1:4708036/63‑1 (MQ=255) gCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGtt < 1:1252993/63‑1 (MQ=255) cAGAAATTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACg < 1:2586905/71‑1 (MQ=255) cAGAAATTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACg < 1:2091757/71‑1 (MQ=255) | GAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACG > W3110S.gb/862181‑862314 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |