Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 862,250 G→T 27.9% T260N (ACT→AAT)  ybiW ← predicted pyruvate formate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb862,2500GT27.9% 89.5 / 16.2 39T260N (ACT→AAT) ybiWpredicted pyruvate formate lyase
Reads supporting (aligned to +/- strand):  ref base G (16/12);  new base T (3/8);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 1.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACG  >  W3110S.gb/862181‑862314
                                                                     |                                                                
gAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGt                                                                 >  1:4180503/1‑71 (MQ=255)
gAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGt                                                                 >  1:2939907/1‑71 (MQ=255)
     aCCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTgcg                                                            >  1:2928988/1‑71 (MQ=255)
       cGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTc                                                                >  1:3002618/1‑65 (MQ=255)
           aGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCt                                                      >  1:4739060/1‑71 (MQ=255)
           aGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCt                                                      >  1:2757309/1‑71 (MQ=255)
           aGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCt                                                      >  1:894781/1‑71 (MQ=255)
              ttCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCt                                                      <  1:3819038/68‑1 (MQ=255)
              ttCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCt                                                      <  1:4917184/68‑1 (MQ=255)
              ttCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCt                                                      <  1:3825116/68‑1 (MQ=255)
               tCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCtggtg                                                  >  1:2371693/1‑71 (MQ=255)
                cGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCtggtgg                                                 >  1:2566181/1‑71 (MQ=255)
                     tGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCt                                                      <  1:3361222/61‑1 (MQ=255)
                     tGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTTGTGGGCg                                              <  1:2079925/69‑1 (MQ=255)
                       cAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCtgg                                                    >  1:1414587/1‑61 (MQ=255)
                       cAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCtgg                                                    >  1:1681076/1‑61 (MQ=255)
                       cAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGAt                                            <  1:1069367/69‑1 (MQ=255)
                       cAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGAt                                            <  1:1075346/69‑1 (MQ=255)
                        aaaaTCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCTGGTGGGc                                               <  1:5083984/65‑1 (MQ=255)
                         aaaTCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGAt                                            >  1:1555455/1‑67 (MQ=255)
                          aaTCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCTGGTGGGCGATAAGAt                                       <  1:3959581/71‑1 (MQ=255)
                                cTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGt                                 <  1:4767379/71‑1 (MQ=255)
                                   gATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGtttt                              <  1:4221449/71‑1 (MQ=255)
                                   gATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGtttt                              <  1:1176643/71‑1 (MQ=255)
                                    aTGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTc                             <  1:1777543/71‑1 (MQ=255)
                                        aGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGc                          >  1:2630387/1‑70 (MQ=255)
                                         gTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGAt                                            <  1:3079561/51‑1 (MQ=255)
                                           aaCACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCtg                                                     >  1:2988339/1‑40 (MQ=255)
                                            acacAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATc                      <  1:4919790/70‑1 (MQ=255)
                                                   tGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGAt                                       >  1:4182726/1‑46 (MQ=255)
                                                   tGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGAt                                       >  1:3331755/1‑46 (MQ=255)
                                                   tGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGAt                                       >  1:896120/1‑46 (MQ=255)
                                                    gCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCcagcatt             >  1:4456941/1‑69 (MQ=255)
                                                     cAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGtt            <  1:944138/71‑1 (MQ=255)
                                                         gcCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCa                                   >  1:3127963/1‑44 (MQ=255)
                                                             gCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGtt            <  1:4708036/63‑1 (MQ=255)
                                                             gCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGtt            <  1:1252993/63‑1 (MQ=255)
                                                               cAGAAATTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACg  <  1:2586905/71‑1 (MQ=255)
                                                               cAGAAATTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACg  <  1:2091757/71‑1 (MQ=255)
                                                                     |                                                                
GAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACG  >  W3110S.gb/862181‑862314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: