| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 891,153 | C→T | 39.0% | R8H (CGT→CAT) | grxA ← | glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 891,153 | 0 | C | T | 39.0% | 31.4 / 42.5 | 41 | R8H (CGT→CAT) | grxA | glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a) |
| Reads supporting (aligned to +/- strand): ref base C (11/14); new base T (9/7); total (20/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
TCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATG > W3110S.gb/891090‑891222 | tcGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCaaa < 1:24328/71‑1 (MQ=255) tCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAAc > 1:4638300/1‑71 (MQ=255) cATTGCTCAATTTCTCAGCCAGATCTTTTG‑‑CGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGt > 1:4126924/1‑71 (MQ=255) gCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACGGttt > 1:4986907/1‑71 (MQ=255) cTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTg < 1:2463214/71‑1 (MQ=255) cTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATa > 1:2345411/1‑63 (MQ=255) tctcAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCattatt > 1:1060522/1‑71 (MQ=255) tctcAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCattatt > 1:4188044/1‑71 (MQ=255) tctcAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCattatt > 1:2893939/1‑71 (MQ=255) tctcAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCattat < 1:623430/70‑1 (MQ=255) tctcAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCattat < 1:3995511/70‑1 (MQ=255) aGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTtctc < 1:1229288/71‑1 (MQ=255) aGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTAttt < 1:2159699/68‑1 (MQ=255) aGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTAttt < 1:4233395/68‑1 (MQ=255) cAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTctcc > 1:1451639/1‑71 (MQ=255) aTCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTtctctc < 1:1147775/67‑1 (MQ=255) gcacgcacACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGAtt < 1:1420551/70‑1 (MQ=255) gcacgcacACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGAtt < 1:254015/70‑1 (MQ=255) acgcacACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTAt < 1:798683/71‑1 (MQ=255) acgcacACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTAt < 1:1357226/71‑1 (MQ=255) acgcacACAGTAAGGGCAACCCGAACAACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTAt < 1:535537/71‑1 (MQ=255) gcacACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGc > 1:2525965/1‑71 (MQ=255) gcacACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGc > 1:3604759/1‑71 (MQ=255) gcacACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGc > 1:2171876/1‑71 (MQ=255) acaGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTg > 1:3164023/1‑70 (MQ=255) acaGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGt < 1:4173868/71‑1 (MQ=255) aGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTaa > 1:4211103/1‑71 (MQ=255) gTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAAt < 1:2341891/71‑1 (MQ=255) gTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAAt < 1:2239555/71‑1 (MQ=255) aaGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGc > 1:3515662/1‑62 (MQ=255) aGGGCAACCCGAACGACCAAAAATAACGGTTTGCattat > 1:1747393/1‑39 (MQ=255) cAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTa < 1:3770950/62‑1 (MQ=255) cccGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCt > 1:321035/1‑70 (MQ=255) cGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCAt > 1:583133/1‑42 (MQ=255) gAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTaaaa > 1:2251341/1‑71 (MQ=255) gAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTaaaa > 1:4852200/1‑71 (MQ=255) gAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTaa > 1:1605793/1‑69 (MQ=255) aaCGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAAt < 1:725667/71‑1 (MQ=255) aCGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAAt < 1:1271888/70‑1 (MQ=255) aCGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATg < 1:3882374/71‑1 (MQ=255) aCGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATg < 1:1959798/71‑1 (MQ=255) cGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATg > 1:1020956/1‑70 (MQ=255) | TCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATG > W3110S.gb/891090‑891222 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |