| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 988,122 | A→G | 6.2% | V429A (GTT→GCT) | asnS ← | asparaginyl tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 988,122 | 0 | A | G | 6.2% | 271.6 / 8.4 | 96 | V429A (GTT→GCT) | asnS | asparaginyl tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (44/46); new base G (4/2); total (48/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01 | |||||||||||
ACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATAC > W3110S.gb/988052‑988191 | acgtacgtTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGaa > 1:1943961/1‑71 (MQ=255) gtacgtTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACa < 1:3676176/71‑1 (MQ=255) gtacgtTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACa < 1:4830644/71‑1 (MQ=255) gtacgtTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACa < 1:335942/71‑1 (MQ=255) tacgtTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAg < 1:4842589/71‑1 (MQ=255) cgtTTTGCACGCCAGTTACGTAAGCAATCATACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACaat < 1:4764261/70‑3 (MQ=255) cgtTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGt < 1:4490685/70‑1 (MQ=255) cgtTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGt < 1:801629/70‑1 (MQ=255) cgtTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGt < 1:1292573/70‑1 (MQ=255) cgtTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTa < 1:2953152/71‑1 (MQ=255) gtTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTAc < 1:5100564/71‑1 (MQ=255) tttGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCg > 1:1790379/1‑71 (MQ=255) tttGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCg > 1:1550100/1‑71 (MQ=255) ttGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTAc < 1:966985/68‑1 (MQ=255) ttGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCg > 1:3764310/1‑70 (MQ=255) ttGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCg > 1:3958577/1‑70 (MQ=255) cacaCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGc < 1:4105887/71‑1 (MQ=255) gCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGAc > 1:4983778/1‑71 (MQ=255) aGTTACTTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCa < 1:1525037/71‑1 (MQ=255) gTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGa < 1:1124323/66‑1 (MQ=255) ttACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGc < 1:4806392/68‑1 (MQ=255) tACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACg > 1:4302290/1‑66 (MQ=255) tACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACg > 1:2647481/1‑66 (MQ=255) tACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACg > 1:3018397/1‑66 (MQ=255) tACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACg > 1:4862830/1‑66 (MQ=255) tACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACg > 1:2009233/1‑66 (MQ=255) tACGTAAGCAATCAGACCTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACg > 1:4174288/1‑66 (MQ=255) gTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAGCAGTACCGTAGCGACGCAGATcgc < 1:3789994/71‑1 (MQ=255) gTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATcgc < 1:4994639/71‑1 (MQ=255) aaGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGa > 1:5084529/1‑71 (MQ=255) aGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGa > 1:3745296/1‑70 (MQ=255) aGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGa > 1:4637914/1‑70 (MQ=255) aGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGa > 1:3670897/1‑70 (MQ=255) aGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGa > 1:4609775/1‑70 (MQ=255) aGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGa > 1:4175773/1‑70 (MQ=255) aGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGAt < 1:4302503/71‑1 (MQ=255) aGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGAt < 1:4192463/71‑1 (MQ=255) gCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGaa > 1:3619597/1‑43 (MQ=255) gCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGaa > 1:18738/1‑43 (MQ=255) gCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAAc > 1:533556/1‑44 (MQ=255) cAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACAGTAGCGACGCAGATc > 1:3208062/1‑64 (MQ=255) tCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGAt < 1:4976828/66‑1 (MQ=255) tCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGAt < 1:3117837/66‑1 (MQ=255) cAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATaccac > 1:2572423/1‑70 (MQ=255) cAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATaccac > 1:1835326/1‑70 (MQ=255) cAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATaccac > 1:4679950/1‑70 (MQ=255) aGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATaccac > 1:4067260/1‑69 (MQ=255) aGACGTTCAAAACCAAGACAGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATaccac > 1:1700904/1‑69 (MQ=255) gACGTTCAAAACCAAGACCGAAACCTGAATGCGGAGCAGTACCGTAGCGACGCAGATCGCGATACCACCAg > 1:4156606/1‑71 (MQ=255) gACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAg < 1:34755/71‑1 (MQ=255) cGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTa < 1:5138101/71‑1 (MQ=255) cGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTa < 1:3885194/71‑1 (MQ=255) gTTCAAAACCAAGACCGAAACCTGAATGCGGAGCAGTACCGTAGCGACGCAGATcgc < 1:3745339/57‑1 (MQ=255) gTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTa < 1:2403017/70‑1 (MQ=255) gTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTa < 1:3903997/70‑1 (MQ=255) ttCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGc < 1:4410871/44‑1 (MQ=255) ttCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGc < 1:3933085/44‑1 (MQ=255) ttCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGc < 1:5000718/44‑1 (MQ=255) ttCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAg < 1:938370/51‑1 (MQ=255) ttCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAg < 1:4216175/51‑1 (MQ=255) ttCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAg < 1:1225954/51‑1 (MQ=255) tCAAAACCAAGACCGAAACCTGAATGCGGAGCAGTACCGTAGCGACGCAGATCGCGa > 1:2650734/1‑57 (MQ=255) tCAAAACCAAGACCGAAACCTGAATGCGGAGCAGTACCGTAGCGACGCAGATCGCGa > 1:328454/1‑57 (MQ=255) tCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAAtc > 1:4105809/1‑71 (MQ=255) cAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATa > 1:3483374/1‑58 (MQ=255) cAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATa > 1:3931149/1‑58 (MQ=255) cAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATa > 1:3159054/1‑58 (MQ=255) cAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAAtct > 1:3838314/1‑71 (MQ=255) cAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAAtct > 1:2094827/1‑71 (MQ=255) aaaaCCAAGACCGAAACCTGAATGCGGAACAGTAc > 1:308684/1‑35 (MQ=255) aaCCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAg < 1:3797800/46‑1 (MQ=255) aaCCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAg < 1:2772649/62‑1 (MQ=255) aCCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATcttctt < 1:4575651/71‑1 (MQ=255) aCCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATcttctt > 1:3737694/1‑71 (MQ=255) ccAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAAtcttct > 1:3341835/1‑69 (MQ=255) cAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATcttctt < 1:2064090/69‑1 (MQ=255) aaGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGc > 1:4725769/1‑40 (MQ=255) aaGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGa > 1:1482687/1‑49 (MQ=255) aaGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGa > 1:1652124/1‑49 (MQ=255) aaGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGa > 1:3000191/1‑49 (MQ=255) aGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTAtt < 1:9831/71‑1 (MQ=255) aGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTAtt < 1:4593764/71‑1 (MQ=255) aGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTAtt < 1:1229553/71‑1 (MQ=255) aGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTAtt < 1:3829282/71‑1 (MQ=255) aGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTAtt < 1:4530714/71‑1 (MQ=255) aaaCCTGAATGCGGAGCAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGc > 1:3508455/1‑70 (MQ=255) aaaCCTGAATGCGGAACAGTACCGTAGCGACGCAGCTCGCGATACCACCAGTAATCTTCTTTATTCAGGc > 1:4903896/1‑70 (MQ=255) aaaCCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGcc > 1:639166/1‑71 (MQ=255) aaaCCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGcc > 1:4740766/1‑71 (MQ=255) aaCCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGccc < 1:3408705/71‑1 (MQ=255) aCCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCa > 1:2747375/1‑71 (MQ=255) aCCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGACCa < 1:818212/71‑1 (MQ=255) aaTGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCAttt < 1:2690976/69‑1 (MQ=255) aaTGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCAttt < 1:157122/69‑1 (MQ=255) aaTGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTc > 1:19030/1‑70 (MQ=255) aaTGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTc > 1:1896541/1‑70 (MQ=255) aaTGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTc > 1:4533229/1‑70 (MQ=255) gAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATa < 1:2002651/71‑1 (MQ=255) gAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATa < 1:4118760/71‑1 (MQ=255) aaCAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATAc > 1:3662484/1‑71 (MQ=255) | ACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATAC > W3110S.gb/988052‑988191 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |