Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	991,514	C→T	8.0%	S157S (TCC→TCT)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	991,514	0	C	T	8.0%	153.3 / 3.6	50	S157S (TCC→TCT)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base C (23/23);  new base T (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

CGGACGTGCTGGCGCGTTTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCC  >  W3110S.gb/991447‑991579                                                                    |                                                                  cGGACGTGCTGGCGCGTTTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAAt                                                                <  1:439502/71‑1 (MQ=255) cGGACGTGCTGGCGCGTTTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAAt                                                                >  1:3569411/1‑71 (MQ=255)                 ttttACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTcc                                                                   <  1:4678307/52‑1 (MQ=255)                   ttACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCTAATGGTAACCGCGTTgcgc                                                >  1:4183983/1‑69 (MQ=255)                   ttACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTgcgc                                                >  1:781199/1‑69 (MQ=255)                     accaccAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTcc                                                                   <  1:3187238/48‑1 (MQ=255)                       caccaAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGc                                           >  1:1526205/1‑70 (MQ=255)                       caccaAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCg                                          <  1:4747976/71‑1 (MQ=255)                        accaAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGa                                         <  1:2304106/71‑1 (MQ=255)                         ccaAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGaa                                        <  1:827403/71‑1 (MQ=255)                          caAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTcc                                                                   <  1:3092357/43‑1 (MQ=255)                           aaaaTTATTGCCGATAAAATCATATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAAc                                       >  1:184098/1‑70 (MQ=255)                           aaaaTTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCaa                                                                 >  1:4549941/1‑44 (MQ=255)                           aaaaTTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGaa                                        >  1:5106292/1‑69 (MQ=255)                            aaaTTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCaa                                                                 >  1:4045645/1‑43 (MQ=255)                            aaaTTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCaa                                                                 >  1:2689634/1‑43 (MQ=255)                            aaaTTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCaa                                                                 >  1:3694833/1‑43 (MQ=255)                            aaaTTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCaa                                                                 >  1:4359387/1‑43 (MQ=255)                            aaaTTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTg                                     >  1:510403/1‑71 (MQ=255)                               ttattGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGaa                                  <  1:2415926/71‑1 (MQ=255)                               ttattGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGaa                                  <  1:3167700/71‑1 (MQ=255)                                tattGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGaa                                  >  1:2978479/1‑70 (MQ=255)                                 attGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGaaa                                 <  1:1574643/70‑1 (MQ=255)                                    gCCGATAAAATCAAATATCCCTTCCTGCTTTTCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACg                              <  1:2986317/70‑1 (MQ=255)                                    gCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACg                              >  1:5093829/1‑70 (MQ=255)                                    gCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACg                              <  1:3175461/70‑1 (MQ=255)                                    gCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACg                              <  1:1823610/70‑1 (MQ=255)                                    gCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACg                              <  1:4504864/70‑1 (MQ=255)                                     ccGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGa                            >  1:1297437/1‑71 (MQ=255)                                      cGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTgc                                                  <  1:527793/48‑1 (MQ=255)                                      cGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTgc                                                  <  1:2459516/48‑1 (MQ=255)                                      cGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTgc                                                  <  1:4898561/48‑1 (MQ=255)                                         tAAAATCAAATATCCCTTCCTGCTTTCTAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGcc                        <  1:2199841/71‑1 (MQ=255)                                          aaaaTCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGaa                                        >  1:2688927/1‑54 (MQ=255)                                          aaaaTCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGaa                                        >  1:1991034/1‑54 (MQ=255)                                          aaaaTCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGaa                                        >  1:4515121/1‑54 (MQ=255)                                          aaaaTCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGaa                                        >  1:4616007/1‑54 (MQ=255)                                          aaaaTCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCa                       >  1:174195/1‑71 (MQ=255)                                            aaTCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACg                              <  1:2281240/62‑1 (MQ=255)                                             aTCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACg                              <  1:1989301/61‑1 (MQ=255)                                             aTCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACg                              <  1:3391328/61‑1 (MQ=255)                                              tCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTgg                   >  1:942775/1‑71 (MQ=255)                                              tCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTgg                   >  1:463102/1‑71 (MQ=255)                                               cAAATATCCCTTCCTGCTTTCTAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTggg                  >  1:5103193/1‑71 (MQ=255)                                                   tatCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACa              <  1:3414881/71‑1 (MQ=255)                                                   tatCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACa              <  1:1554961/71‑1 (MQ=255)                                                     tCCCTTCCTGCTTTCTAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGt            <  1:4113888/71‑1 (MQ=255)                                                       ccTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTg           <  1:2738084/70‑1 (MQ=255)                                                         ttCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGaa                                  <  1:131007/45‑1 (MQ=255)                                                           ccTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAg       >  1:1659682/1‑70 (MQ=255)                                                            cTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAgg      <  1:598088/70‑1 (MQ=255)                                                               cTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGAccc  >  1:1361756/1‑71 (MQ=255)                                                                  tCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACTCCa                       <  1:796941/47‑1 (MQ=255)                                                                    |                                                                  CGGACGTGCTGGCGCGTTTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCC  >  W3110S.gb/991447‑991579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑