| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,007,501 | A→C | 35.0% | N174K (AAT→AAG) | ycbX ← | predicted 2Fe‑2S cluster‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,007,501 | 0 | A | C | 35.0% | 11.0 / 48.2 | 40 | N174K (AAT→AAG) | ycbX | predicted 2Fe‑2S cluster‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (14/12); new base C (6/8); total (20/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGGAGATCACGTAACGAGGCT > W3110S.gb/1007432‑1007567 | caccacATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGAt < 1:1527603/71‑1 (MQ=255) cacaTCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTgg < 1:525987/71‑1 (MQ=255) tCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGc < 1:4316079/70‑1 (MQ=255) cACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCg > 1:337911/1‑70 (MQ=255) aCCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCgg > 1:325283/1‑70 (MQ=255) ccAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCTCCGGAAACCACCAGCTTGGGGCGGaa < 1:4658556/71‑1 (MQ=255) ccAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGaa > 1:1356521/1‑71 (MQ=255) ccAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGaa < 1:4276563/71‑1 (MQ=255) ccAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGaa > 1:767905/1‑71 (MQ=255) aaTGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAAtt < 1:3102280/71‑1 (MQ=255) aTGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCTGAAACCACCAGATTGGGGCGGAATTg < 1:1452175/71‑1 (MQ=255) aTGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGAt > 1:2198792/1‑58 (MQ=255) aTGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTg < 1:2765304/71‑1 (MQ=255) aTGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTg < 1:1154526/71‑1 (MQ=255) cGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGaa > 1:1311806/1‑65 (MQ=255) cGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGaa > 1:715272/1‑65 (MQ=255) cGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTc > 1:1494649/1‑71 (MQ=255) tCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCAtt < 1:3004337/71‑1 (MQ=255) acACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCAtt > 1:4917561/2‑71 (MQ=255) aCTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAGACCACCAGCTTGGGGCGGAATTGCTCCAtttt < 1:1811373/71‑1 (MQ=255) aCTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCAtttt < 1:1714794/71‑1 (MQ=255) tCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCATTTTTaca > 1:2580060/1‑71 (MQ=255) cAGCGATCTTCATCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCATTTTTaca > 1:4674535/1‑69 (MQ=255) gCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCATTTTTACACTgg > 1:4791218/1‑71 (MQ=255) cGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCATTTTTa > 1:4842491/1‑64 (MQ=255) cttcttCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCAt < 1:4001174/55‑1 (MQ=255) ttcttcCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCATTTTTa < 1:3357386/59‑1 (MQ=255) ttcttcCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCATTTTTACACTg < 1:2210236/64‑1 (MQ=255) ccAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACgt < 1:1069450/70‑1 (MQ=255) cAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACgt > 1:4425985/1‑69 (MQ=255) gCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCATTTTTaca > 1:1706811/1‑51 (MQ=255) cTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTgg < 1:1512454/71‑1 (MQ=255) cTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTgg < 1:1550265/71‑1 (MQ=255) tGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGttgttg > 1:440797/1‑69 (MQ=255) tGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGttgttg > 1:4173608/1‑69 (MQ=255) tGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCATTTTTAAACTGGCCGGACAACGttgttg > 1:1809322/1‑69 (MQ=255) ccGGAAACCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACCACGTTGTTGGAGATCAc < 1:5066995/70‑1 (MQ=255) aaCCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGGAGATCACGTAAc > 1:2719194/1‑70 (MQ=255) caccaGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGgag < 1:4270289/57‑1 (MQ=255) ccaGATTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGGAGATCACGTAACGAGGCt > 1:5010264/1‑71 (MQ=255) | CACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGGAGATCACGTAACGAGGCT > W3110S.gb/1007432‑1007567 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |