| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,008,134 | C→T | 33.6% | intergenic (‑112/‑132) | ycbX ← / → ycbY | predicted 2Fe‑2S cluster‑containing protein/predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,008,134 | 0 | C | T | 33.6% | 65.4 / 51.2 | 57 | intergenic (‑112/‑132) | ycbX/ycbY | predicted 2Fe‑2S cluster‑containing protein/predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (19/18); new base T (11/8); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
ATGACGCGCTTATTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTTCTCCGCGCTGT > W3110S.gb/1008064‑1008200 | atgacGCGCTTATTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTc < 1:1187843/71‑1 (MQ=255) acGCGCTTATTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGt < 1:4025591/71‑1 (MQ=255) cgcTTATTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTg < 1:2778602/71‑1 (MQ=255) gcTTATTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTgg < 1:1696985/71‑1 (MQ=255) tattaGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTg > 1:3849804/1‑67 (MQ=255) attaGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGcc < 1:4348167/69‑1 (MQ=255) gCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCt < 1:2708474/67‑1 (MQ=255) gCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGt < 1:2293772/71‑1 (MQ=255) tataATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAg > 1:3032136/1‑56 (MQ=255) ataATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTAt > 1:814029/1‑71 (MQ=255) taATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGt < 1:5101648/67‑1 (MQ=255) aTGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCt > 1:3184445/1‑71 (MQ=255) aTGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTa > 1:2102095/1‑67 (MQ=255) cgcAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGttt > 1:1934880/1‑52 (MQ=255) cgcAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCg > 1:1012922/1‑70 (MQ=255) caacaaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTTTCAGTTTGGCCCTGGGTTATGCTCGaa < 1:3497649/70‑1 (MQ=255) caacaaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGaaa > 1:4151652/1‑71 (MQ=255) aacaaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGt < 1:430076/59‑1 (MQ=255) aacaaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGaa < 1:1093921/69‑1 (MQ=255) aacaaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGaa < 1:3037335/69‑1 (MQ=255) aacaaTTTTCTTAGCGCCTATCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGaaa > 1:2139169/1‑70 (MQ=37) caaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATcc > 1:2684323/1‑71 (MQ=255) caaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATcc > 1:2329050/1‑71 (MQ=255) aaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCt < 1:3577178/71‑1 (MQ=255) aTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCt > 1:4260428/1‑70 (MQ=255) aTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAg < 1:3921896/42‑1 (MQ=255) aTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAg < 1:4939952/42‑1 (MQ=255) tttCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCa > 1:3456788/1‑70 (MQ=255) tCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACg > 1:2320105/1‑70 (MQ=255) tCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACg > 1:964603/1‑70 (MQ=255) tCTTAGAGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACg > 1:264/1‑70 (MQ=255) tttAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGcc > 1:4215474/2‑44 (MQ=255) cTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGcc > 1:827771/1‑44 (MQ=255) cTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGcc > 1:821909/1‑44 (MQ=255) cTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGt < 1:898828/70‑1 (MQ=255) cTTAGCCCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGcc > 1:4737610/1‑44 (MQ=255) gcgcCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACtt < 1:779189/70‑1 (MQ=255) gcCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtgtg < 1:3324947/71‑1 (MQ=255) gcCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtgtg < 1:1669435/71‑1 (MQ=255) gcCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtgtg < 1:4700520/71‑1 (MQ=255) gcCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtgtg < 1:2314171/71‑1 (MQ=255) tGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtgtg < 1:421538/68‑1 (MQ=255) tCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTa > 1:3353185/1‑68 (MQ=255) ttAGGCTATTTTATTTGT‑AGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGt < 1:2662940/71‑1 (MQ=255) ttAGGCTACTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCt < 1:5018745/49‑1 (MQ=255) aGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGtt > 1:2849428/1‑71 (MQ=255) ggCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGttt < 1:2625425/71‑1 (MQ=255) ttttatttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTgtgt < 1:465069/54‑1 (MQ=255) ttttatttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTTCTCcg > 1:4578462/1‑71 (MQ=255) tttatttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTTCTCcgc > 1:3101214/1‑71 (MQ=255) ttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACg > 1:4725798/1‑55 (MQ=255) ttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTTCTCcgcg > 1:2982798/1‑71 (MQ=255) ttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTTCTCcgc < 1:1694421/70‑1 (MQ=255) ttatttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTAc > 1:1872643/1‑54 (MQ=255) ttatttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTAc > 1:3550052/1‑54 (MQ=255) ttatttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTTCTCcgcg > 1:1763249/1‑71 (MQ=255) tGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCg > 1:1754142/1‑55 (MQ=255) gTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTTCTCCGCGCtgt < 1:2243965/69‑1 (MQ=255) | ATGACGCGCTTATTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTTCTCCGCGCTGT > W3110S.gb/1008064‑1008200 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |