| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,028,434 | G→A | 47.4% | D23N (GAC→AAC) | yccU → | predicted CoA‑binding protein with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,028,434 | 0 | G | A | 47.4% | 3.4 / 51.6 | 38 | D23N (GAC→AAC) | yccU | predicted CoA‑binding protein with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (11/9); new base A (8/10); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
TGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTC > W3110S.gb/1028369‑1028500 | tGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCTCTGGTGGGGGCAAGCGACaaa < 1:184235/71‑1 (MQ=255) agaaaCCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCg < 1:4408287/71‑1 (MQ=255) tGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTc < 1:2922302/65‑1 (MQ=255) tGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTc < 1:4553907/65‑1 (MQ=255) tGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTc < 1:1709150/65‑1 (MQ=255) gCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTCCCAGCt < 1:3224917/70‑1 (MQ=255) gCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTCCCAGCt < 1:3367396/70‑1 (MQ=255) gCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTCCCAGCt < 1:1704848/70‑1 (MQ=255) gCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTCCCAGCTa > 1:1453029/1‑71 (MQ=255) gCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTCCCAGCTa > 1:2250681/1‑71 (MQ=255) cAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGAc > 1:2589795/1‑50 (MQ=255) cAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAAcc > 1:87944/1‑55 (MQ=255) aTTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGt < 1:3474761/71‑1 (MQ=255) aTTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTCCCAGCTATCGCGt < 1:4443685/71‑1 (MQ=255) ttttAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCg < 1:3460427/51‑1 (MQ=255) ttttAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCg < 1:1577054/51‑1 (MQ=255) ttttAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTCCCAGCTATCGCGt < 1:3228281/70‑1 (MQ=255) aCGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGaa < 1:2525473/71‑1 (MQ=255) ccACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGAGTGATGAAatat > 1:3981462/1‑71 (MQ=255) accccATTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTc > 1:1637816/4‑71 (MQ=255) acacCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCGCAGCTATCGCGTGATGaaa > 1:3216784/1‑62 (MQ=255) acacCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGaaa > 1:1220710/1‑62 (MQ=255) acacCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAata > 1:2800316/1‑64 (MQ=255) acacCATTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACt > 1:3768784/1‑70 (MQ=255) cacCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCg < 1:4001362/71‑1 (MQ=255) cacCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCg < 1:880332/71‑1 (MQ=255) cacCATTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTc > 1:98451/1‑70 (MQ=255) ccATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTa > 1:191609/1‑65 (MQ=255) aTTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCa < 1:304737/71‑1 (MQ=255) tggtggGGGCAAGCAACAAACCCGATCGTCCCAGCTATCGCGt < 1:4926130/43‑1 (MQ=255) tggtggGGGCAAGCAACAAACCCGATCGTCCCAGCTATCGCGt < 1:4871706/43‑1 (MQ=255) gggggCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGaaa > 1:1629799/1‑46 (MQ=255) aaGCAACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTc > 1:4242880/1‑71 (MQ=255) aaGCAACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTc > 1:2131447/1‑71 (MQ=255) aaGCAACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTc > 1:4167258/1‑71 (MQ=255) gCGACAAACCCGATCGTCCCAGCTATCGCGTGTTGAAATATCTACTCGACCAg > 1:4947420/1‑53 (MQ=255) gCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGa > 1:4670248/1‑49 (MQ=255) gCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGa > 1:3175241/1‑49 (MQ=255) | TGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTC > W3110S.gb/1028369‑1028500 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |