| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,084,947 | A→G | 51.1% | K384E (AAA→GAA) | ycdB → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,084,947 | 0 | A | G | 51.1% | 0.2 / 67.9 | 47 | K384E (AAA→GAA) | ycdB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (12/11); new base G (10/14); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01 | |||||||||||
TGGATATGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGCC > W3110S.gb/1084882‑1085017 | tGGATATGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACaagaaa > 1:2312258/1‑69 (MQ=255)tGGATATGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAgg > 1:1646075/1‑71 (MQ=255) atatGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTc < 1:2573672/71‑1 (MQ=255) atatGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTc < 1:229799/71‑1 (MQ=255) atatGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTc < 1:4172467/71‑1 (MQ=255) tatGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTc < 1:2637092/70‑1 (MQ=255) tatGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTc < 1:2631412/70‑1 (MQ=255) ttGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACaaaa < 1:1682434/56‑1 (MQ=255) ttGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACaaaa < 1:4983390/56‑1 (MQ=255) ttGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTc < 1:3935645/63‑1 (MQ=255) tGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCg > 1:3193155/1‑69 (MQ=255) gCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGGTTCCTGACAGTACAAAAAAGGCTc < 1:5141823/61‑1 (MQ=255) gCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAg > 1:4281119/1‑71 (MQ=255) gCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTc < 1:2078959/61‑1 (MQ=255) tttGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAgcg < 1:3952342/69‑1 (MQ=255) tttGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAg < 1:4619023/53‑1 (MQ=255) tttGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAg < 1:3894400/53‑1 (MQ=255) ttGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTg > 1:2376015/1‑71 (MQ=255) tGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACaaaaaa > 1:413407/1‑50 (MQ=255) tGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACaaaaaa > 1:536970/1‑50 (MQ=255) tGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGaa > 1:3814607/1‑64 (MQ=255) tGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCt > 1:5070133/1‑69 (MQ=255) tGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTg > 1:4327661/1‑70 (MQ=255) tGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCaa > 1:2237900/1‑57 (MQ=255) tGTCTGCTA‑CAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGaa > 1:2380093/1‑63 (MQ=255) gTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTgg < 1:4315024/70‑1 (MQ=255) tACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTc < 1:871412/48‑1 (MQ=255) tACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTc < 1:1151061/48‑1 (MQ=255) ccAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATa < 1:4519740/69‑1 (MQ=255) aaCACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTgg > 1:2928167/1‑60 (MQ=255) aaCACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGt > 1:2127940/1‑70 (MQ=255) acacGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATgg < 1:2642614/48‑1 (MQ=255) acacGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGACGCGCTGGAGGAATACGtt > 1:4097586/1‑70 (MQ=255) acacGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTa < 1:197098/71‑1 (MQ=255) cacGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTaa < 1:2354104/71‑1 (MQ=255) acGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCg > 1:3245447/1‑48 (MQ=255) acGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTaaa > 1:3448209/1‑71 (MQ=255) cGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATgg > 1:3482180/1‑45 (MQ=255) cGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAAc < 1:3904196/71‑1 (MQ=255) aTCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTc < 1:3800341/38‑1 (MQ=255) tGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTaaa > 1:2368997/1‑65 (MQ=255) gAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcgg > 1:1399858/1‑71 (MQ=255) aaaaaGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTa < 1:3482249/66‑1 (MQ=255) aaaaaGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcggc < 1:595813/71‑1 (MQ=255) aaaaaGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcggc < 1:1268420/71‑1 (MQ=255) aaaaGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTgga > 1:727893/1‑48 (MQ=255) aCAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTAtttttt > 1:3925534/1‑71 (MQ=255) tACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTaaa < 1:2948337/44‑1 (MQ=255) aCAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTAt > 1:189825/1‑48 (MQ=255) cAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTAtttt < 1:2308732/63‑1 (MQ=255) cAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAAc > 1:3750867/1‑43 (MQ=255) cAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCt > 1:1757313/1‑71 (MQ=255) aaaaaGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGc > 1:3050150/1‑71 (MQ=255) aaaaaGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGc < 1:1438748/71‑1 (MQ=255) aaaaGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGcc < 1:1339084/71‑1 (MQ=255) | TGGATATGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGCC > W3110S.gb/1084882‑1085017 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |