Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,097,861	G→A	51.7%	noncoding (33/88 nt)	serX ←	tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,097,861	0	G	A	51.7%	‑4.6 / 41.7	29	noncoding (33/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (4/10);  new base A (6/9);  total (10/19)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

CCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGC  >  W3110S.gb/1097832‑1097926                              |                                                                  ccGATACGTTGCCGTATACACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCg                          >  1:845708/1‑71 (MQ=17)     tACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTcc                      <  1:2170911/71‑1 (MQ=39)     tACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTcc                      <  1:2267643/71‑1 (MQ=39)      aCGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCt                     >  1:2570212/1‑71 (MQ=40)       cGTTGCCGTATACACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTg                    <  1:4033536/71‑1 (MQ=255)       cGTTGCCGTATACACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTg                    >  1:1033438/1‑71 (MQ=255)       cGTTGCCGTATACACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTg                    <  1:443192/71‑1 (MQ=255)         ttGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTc                  <  1:820104/71‑1 (MQ=255)           gCCGTATACACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCtt                >  1:3394494/1‑71 (MQ=255)           gCCGTATACACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCAACAAATTGTCGTTCCTGTCtt                >  1:2198021/1‑71 (MQ=17)            ccGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTg               <  1:2755418/71‑1 (MQ=255)              gTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCt             <  1:3909141/71‑1 (MQ=255)               tataCACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTg                    <  1:1426775/63‑1 (MQ=255)                ataCACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTg            >  1:4526188/1‑70 (MQ=255)                 taCACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTgg           >  1:3207747/1‑70 (MQ=255)                 taCACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGa          <  1:3582454/71‑1 (MQ=255)                 taCACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGa          <  1:4404426/71‑1 (MQ=255)                 taCACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTcc                      <  1:535326/59‑1 (MQ=255)                 taCACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTcc                      <  1:341885/59‑1 (MQ=255)                 taCACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTcc                      <  1:1218388/59‑1 (MQ=255)                 taCACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTgg           <  1:4585499/70‑1 (MQ=255)                   cacacTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAAc        <  1:2530177/71‑1 (MQ=255)                   cacacTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAAc        <  1:2701992/71‑1 (MQ=255)                    acacTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACg       <  1:2179898/71‑1 (MQ=255)                        tttCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTg               <  1:4884188/59‑1 (MQ=255)                        tttCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt                   <  1:2950727/55‑1 (MQ=255)                          tCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCt             >  1:4510771/1‑59 (MQ=255)                          tCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGgcgc  >  1:626681/1‑70 (MQ=255)                           ccAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGgcgc  >  1:3970063/1‑69 (MQ=255)                              |                                                                  CCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGC  >  W3110S.gb/1097832‑1097926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑