| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,099,547 | C→T | 47.5% | A143V (GCC→GTC) | ycdX → | predicted zinc‑binding hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,099,547 | 0 | C | T | 47.5% | 9.9 / 82.5 | 59 | A143V (GCC→GTC) | ycdX | predicted zinc‑binding hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (17/14); new base T (17/11); total (34/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.80e-01 | |||||||||||
CTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTC > W3110S.gb/1099478‑1099613 | cTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGcc < 1:2689715/71‑1 (MQ=255)cTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGcc < 1:4291345/71‑1 (MQ=255) aTCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGt > 1:5078934/1‑71 (MQ=255) tCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGtt > 1:75379/1‑71 (MQ=255) aTGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGcc < 1:3449716/71‑1 (MQ=255) aTGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGcc < 1:779048/71‑1 (MQ=255) aTGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGcc < 1:1445239/71‑1 (MQ=255) aTGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGcc < 1:2659383/71‑1 (MQ=255) tgCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCg < 1:3581478/54‑1 (MQ=255) tgCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCcgc > 1:1796981/1‑70 (MQ=255) cATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGaa < 1:1573273/71‑1 (MQ=255) cATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGa < 1:802690/70‑1 (MQ=255) ataataAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGa < 1:1885757/56‑1 (MQ=255) taataaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTg > 1:643167/1‑51 (MQ=255) aataaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAagc > 1:1920996/1‑57 (MQ=255) aataaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAagc > 1:3737956/1‑57 (MQ=255) ataaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTg > 1:1819146/1‑49 (MQ=255) ataaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAagcagc > 1:237207/1‑59 (MQ=255) ataaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATc < 1:1215645/70‑1 (MQ=255) taaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGc > 1:4633102/1‑49 (MQ=255) taaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGaaa > 1:219448/1‑65 (MQ=255) taaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGaaa > 1:4791473/1‑65 (MQ=255) taaGCCATCCCGGAAATCCCAAATATGAAATAGATATGAAAGCCGTTGc > 1:3843036/1‑49 (MQ=255) aaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGc > 1:3977592/1‑42 (MQ=255) tCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGa < 1:2799441/45‑1 (MQ=255) ccGGAAATCCCAAATATGAAATAGATGTGAAAGTCGtt > 1:592088/1‑38 (MQ=255) ccGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAagcagc > 1:72726/1‑49 (MQ=255) ccGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTg > 1:1149638/1‑39 (MQ=255) ccGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCt < 1:830305/70‑1 (MQ=255) ccGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCt < 1:3596424/70‑1 (MQ=255) ccGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTg > 1:4696637/1‑71 (MQ=255) aaTCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAagc > 1:2378819/1‑41 (MQ=255) aTCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAg > 1:573788/1‑56 (MQ=255) aTCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAg > 1:1469327/1‑56 (MQ=255) ccAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGaa < 1:3534648/65‑1 (MQ=255) ccAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCaat < 1:666781/71‑1 (MQ=255) ccAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCaat < 1:1299359/71‑1 (MQ=255) ccAAATATGAAATAGATGTGAAAGCCGTTGCTGAagca > 1:3221270/1‑38 (MQ=255) aaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCaataat > 1:4247066/1‑71 (MQ=255) aaTATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCaataat > 1:1721463/1‑71 (MQ=255) aaTATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCaataa < 1:3356597/70‑1 (MQ=255) atatGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCaat < 1:2739935/67‑1 (MQ=255) tGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTcc < 1:1334148/70‑1 (MQ=255) tGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTcc < 1:1598486/70‑1 (MQ=255) aaTAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCaat < 1:3281552/61‑1 (MQ=255) aaTAGATGTGAAAGTCGTTGCTGAAGCAGC‑‑CGAAACATCAGGTGGCGCTGGAAATCaat < 1:4647155/59‑1 (MQ=255) gATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCaat < 1:4679788/57‑1 (MQ=255) aTGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCaa > 1:992394/1‑55 (MQ=255) aTGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCaa > 1:4957326/1‑55 (MQ=255) aTGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCaa > 1:403586/1‑55 (MQ=255) aTGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCaa > 1:366893/1‑55 (MQ=255) aTGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCaa > 1:2999711/1‑55 (MQ=255) aTGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTa < 1:2647427/71‑1 (MQ=255) gtgAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTcc > 1:381591/1‑60 (MQ=255) gtgAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTcc > 1:2909745/1‑60 (MQ=255) gtgAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTcc > 1:3139310/1‑60 (MQ=255) gtgAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTaca > 1:4374772/1‑71 (MQ=255) tgAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCaataa < 1:2380978/55‑1 (MQ=255) gAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTac > 1:230132/1‑68 (MQ=255) aaaGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACt > 1:29063/1‑70 (MQ=255) aaaGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACt > 1:3702885/1‑70 (MQ=255) aaaGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTc < 1:1126492/71‑1 (MQ=255) | CTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTC > W3110S.gb/1099478‑1099613 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |