| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,112,202 | T→C | 27.3% | F431L (TTT→CTT) | mdoG → | glucan biosynthesis protein, periplasmic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,112,202 | 0 | T | C | 27.3% | 68.3 / 29.7 | 44 | F431L (TTT→CTT) | mdoG | glucan biosynthesis protein, periplasmic |
| Reads supporting (aligned to +/- strand): ref base T (18/14); new base C (3/9); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.36e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAGCGCAAACCAGCATTGGTGA > W3110S.gb/1112134‑1112269 | cAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGAttt < 1:3389774/70‑1 (MQ=255)cAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGAttt < 1:641376/70‑1 (MQ=255)cAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGAttt < 1:2432232/70‑1 (MQ=255) aaCGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGAtctt < 1:467208/70‑1 (MQ=255) tgAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCaga < 1:1437196/70‑1 (MQ=255) aGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGaa > 1:2467598/1‑70 (MQ=255) gTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGAt > 1:169750/1‑66 (MQ=255) gTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGaaaa < 1:4049640/71‑1 (MQ=255) gTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGaaaa < 1:353601/71‑1 (MQ=255) tCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGaaaa < 1:2684834/70‑1 (MQ=255) gAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGaaaaaa < 1:2847826/70‑1 (MQ=255) aaCCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATCTTACCGGCGCAGAGATGAAAAAACt < 1:1034143/71‑1 (MQ=255) aCCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCgg > 1:2983977/1‑52 (MQ=255) aCCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTg > 1:1215244/1‑71 (MQ=255) aCCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTg > 1:5076162/1‑71 (MQ=255) aCCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACAGGCGCAGAGATGAAAAAAc > 1:84940/1‑69 (MQ=255) aCCTGATTCGCAAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCgg > 1:4401525/1‑52 (MQ=255) ccTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGc > 1:3110849/1‑71 (MQ=255) ccTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATCTTACCGGCGCAGAGATGaaaa < 1:3474241/65‑1 (MQ=255) ccTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATCTTACCGGCGCAGAGATGaaaa < 1:2584547/65‑1 (MQ=255) ccTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATCTTACCGGCGCAGAGATGaaaa < 1:3396266/65‑1 (MQ=255) gATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGAttt < 1:2916193/42‑1 (MQ=255) gATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGAttt < 1:678754/42‑1 (MQ=255) gATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGAttt < 1:3345850/42‑1 (MQ=255) gATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGAttt < 1:4370902/42‑1 (MQ=255) ttCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCagag < 1:4953905/71‑1 (MQ=255) ttCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATCTTACCGGCGCAGAGATGAAAAAACTGCCagag < 1:1830468/71‑1 (MQ=255) cGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATCTTACCGGCGCAGAGATGAAAAAACTGCCagag < 1:2718676/69‑1 (MQ=255) cAGCCTGACGGTACTATCGCCTTTGTGTTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATAc < 1:2471712/71‑1 (MQ=255) aGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATAcc > 1:2114527/1‑71 (MQ=255) cTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCg > 1:1672584/1‑71 (MQ=255) aCGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCa < 1:4282054/38‑1 (MQ=255) aCGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGt < 1:3274247/70‑1 (MQ=255) aCGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGt < 1:657983/70‑1 (MQ=255) ggTACTATCGCCTTTGTGGTCGATCTTACCGGCGCAGAGATGaaa > 1:3638247/1‑45 (MQ=255) ggTACTATCGCCTTTGTGGTCGATCTTACCGGCGCAGAGATGaaa > 1:4588419/1‑45 (MQ=255) tATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGc < 1:2185853/47‑1 (MQ=255) tATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGc < 1:4761383/47‑1 (MQ=255) aTCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGc < 1:2825155/46‑1 (MQ=255) tCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGaaaaaa > 1:4897951/1‑41 (MQ=255) tCGCCTTTGTGGTCGATCTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAGCGCa > 1:868746/1‑70 (MQ=255) gCCTTTGTGGTCGATTTTACCGGCGCAGAGATGaaaaaa > 1:3327073/1‑39 (MQ=255) gCCTTTGTGGTCGATTTTACCGGCGCAGAGATGaaaaa > 1:1522742/1‑38 (MQ=255) gCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAGCGCAAAc > 1:1676507/1‑71 (MQ=255) cTTTGTGGTCGATCTTACCGGCGCAGAGATGAAAAAACTGCCagag < 1:145093/46‑1 (MQ=255) ttGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAGCGCAAACCAg > 1:3557469/1‑70 (MQ=255) tgtgGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAGCGCAAACCAg < 1:4960193/69‑1 (MQ=255) tgGTCGATCTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAGCGCAAACCAGCAtt < 1:2161783/71‑1 (MQ=255) gTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAGCGCAAACCAGCATTgg > 1:515034/1‑71 (MQ=255) cGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAGCGCAAACCAGCATTg > 1:1835259/1‑68 (MQ=255) gATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAGCGCAAACCAGCATTGGTGa > 1:236554/1‑71 (MQ=255) gATCTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGt < 1:2236997/47‑1 (MQ=255) | CAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAGCGCAAACCAGCATTGGTGA > W3110S.gb/1112134‑1112269 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |