| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,115,786 | T→C | 30.2% | intergenic (‑28/+55) | msyB ← / ← mdtG | hypothetical protein/predicted drug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,115,786 | 0 | T | C | 30.2% | 67.0 / 44.1 | 53 | intergenic (‑28/+55) | msyB/mdtG | hypothetical protein/predicted drug efflux system |
| Reads supporting (aligned to +/- strand): ref base T (25/12); new base C (10/6); total (35/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01 | |||||||||||
CAGCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATA > W3110S.gb/1115719‑1115851 | cAGCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGctt < 1:1900177/70‑1 (MQ=255) tCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAggg > 1:1326192/1‑70 (MQ=255) aaTGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGaa > 1:2180624/1‑70 (MQ=255) ggCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGttt > 1:1094676/1‑60 (MQ=255) gCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGa < 1:740169/71‑1 (MQ=255) cttcAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGc > 1:1377426/1‑71 (MQ=255) cttcAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGa > 1:2102754/1‑67 (MQ=255) gCGTTGAGTACATGGTCATCACGATTCTCCCTTTGAGTTTATGAGGtt < 1:2332265/48‑1 (MQ=255) gCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCg < 1:2113867/70‑1 (MQ=255) gCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCg < 1:2762100/70‑1 (MQ=255) gTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAg < 1:787388/52‑1 (MQ=255) tACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGaaaa < 1:46822/50‑1 (MQ=255) cATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAgg > 1:1002304/1‑50 (MQ=255) cATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAgg > 1:5058286/1‑50 (MQ=255) cATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAgg > 1:2696057/1‑50 (MQ=255) cATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAgg > 1:4321746/1‑50 (MQ=255) cATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTg > 1:3976345/1‑71 (MQ=255) cATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGaaa > 1:1175978/1‑47 (MQ=255) gTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGaaa > 1:2760264/1‑43 (MQ=255) gTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGaaa > 1:4707801/1‑43 (MQ=255) gTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAg > 1:2422353/1‑71 (MQ=255) atcaCGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCtttt > 1:1462803/1‑63 (MQ=255) atcaCGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTAt > 1:728359/1‑71 (MQ=255) tcaCGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGa < 1:2028893/37‑1 (MQ=255) caCGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATg > 1:1749968/1‑70 (MQ=255) caCGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATg > 1:4891541/1‑70 (MQ=255) caCGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATg > 1:1709964/1‑70 (MQ=255) aCGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGaaa > 1:1180686/1‑37 (MQ=255) aCGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGa < 1:4639689/70‑1 (MQ=255) aCGATTCTCCCTTTGAGTTGATGAGGTTTCACGGaaa > 1:374205/1‑37 (MQ=255) cGATTCTCCCTTTGAGTTGATTAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGaaa < 1:1786146/71‑1 (MQ=255) gATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAg < 1:2958171/71‑1 (MQ=255) aTTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAgg > 1:1085198/1‑71 (MQ=255) aTTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAgg > 1:515971/1‑71 (MQ=255) ttCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGc > 1:1863492/1‑71 (MQ=255) ttCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGc > 1:361067/1‑71 (MQ=255) tctcCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGa > 1:2968357/1‑36 (MQ=255) tctcCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGc > 1:1757394/1‑70 (MQ=255) ttGAGTTGATGATGTTTCAGGGAAAAGGAATGCTGATTCTCCg < 1:4191813/43‑1 (MQ=37) ttGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAAt > 1:794401/1‑70 (MQ=255) tGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAAt > 1:4749096/1‑69 (MQ=255) tGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAAt > 1:107524/1‑69 (MQ=255) gAGTTGATGAGGTTTCATGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAg > 1:1658476/1‑71 (MQ=255) gAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAg > 1:4506949/1‑71 (MQ=255) gAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAg > 1:156742/1‑71 (MQ=255) aGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAg < 1:607570/70‑1 (MQ=255) ttGATGAGGTTTCAGGGAAAAGGATAGCTGATtct < 1:1258341/35‑1 (MQ=255) ttGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTc < 1:3080884/71‑1 (MQ=255) tgatgaGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGa < 1:4107447/52‑1 (MQ=255) tgatgaGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGa < 1:3373198/52‑1 (MQ=255) tgatgaGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGa < 1:211535/52‑1 (MQ=255) tgatgaGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGa < 1:1786323/52‑1 (MQ=255) gatgaGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGa > 1:1477691/1‑71 (MQ=255) gatgaGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGa > 1:118354/1‑71 (MQ=255) tgaGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGa < 1:3816537/49‑1 (MQ=255) tgaGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATa > 1:2689671/1‑71 (MQ=255) | CAGCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATA > W3110S.gb/1115719‑1115851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |