| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,157,831 | G→A | 15.0% | A165T (GCG→ACG) | holB → | DNA polymerase III, delta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,157,831 | 0 | G | A | 15.0% | 103.7 / 9.9 | 40 | A165T (GCG→ACG) | holB | DNA polymerase III, delta prime subunit |
| Reads supporting (aligned to +/- strand): ref base G (17/17); new base A (2/4); total (19/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
TCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCA > W3110S.gb/1157763‑1157896 | tCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTgcg > 1:582582/1‑71 (MQ=255) aCGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCg > 1:998028/1‑71 (MQ=255) aCGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCg > 1:3501146/1‑71 (MQ=255) ttACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGAc > 1:4012151/1‑71 (MQ=255) tACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTg < 1:4034881/68‑1 (MQ=255) tACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGAcc > 1:1028860/1‑71 (MQ=255) aCTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCt > 1:494759/1‑71 (MQ=255) cTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTACGCCGCCGCCGGAACAGTACGCCGTGACCTg > 1:5012840/1‑71 (MQ=255) gCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCt > 1:2334165/1‑71 (MQ=255) aCATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCttt < 1:2601232/70‑1 (MQ=255) aCGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGaa > 1:1134105/1‑43 (MQ=255) cGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGc > 1:4606661/1‑50 (MQ=255) cGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCg > 1:340853/1‑52 (MQ=255) cGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCAcgcg < 1:2044117/70‑1 (MQ=255) cGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCAcgcg < 1:1239252/70‑1 (MQ=255) cGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCAcgcg < 1:1269899/70‑1 (MQ=255) cGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCAcgcg < 1:4006749/70‑1 (MQ=255) gTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGAc > 1:2048562/1‑70 (MQ=255) gTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGAc < 1:612575/70‑1 (MQ=255) gTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGAc > 1:1202461/1‑70 (MQ=255) tGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAAt > 1:1817180/1‑71 (MQ=255) tCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGt < 1:331944/71‑1 (MQ=255) ggTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTca < 1:2265357/71‑1 (MQ=255) aCATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAg < 1:3433021/70‑1 (MQ=255) aCATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAg < 1:1232730/70‑1 (MQ=255) aCATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAg < 1:394411/70‑1 (MQ=255) aCATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAg > 1:3835970/1‑70 (MQ=255) aCATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAg < 1:1464866/70‑1 (MQ=255) aTTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAg < 1:3028222/68‑1 (MQ=255) aTTACCTTACGCCGCCGCCGGAACAGTACGCCGTGACCTGGCttt < 1:247274/45‑1 (MQ=255) aTTACCTTACGCCGCCGCCGGAACAGTACGCCGTGACCTGGCttt < 1:3847792/45‑1 (MQ=255) aTTACCTTACGCCGCCGCCGGAACAGTACGCCGTGACCTGGCttt < 1:2713061/45‑1 (MQ=255) aTTACCTTACGCCGCCGCCGGAACAGTACGCCGTGACCTGGCttt < 1:2702706/45‑1 (MQ=255) ttACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATg > 1:2360906/1‑71 (MQ=255) ttACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATg > 1:4147814/1‑71 (MQ=255) tACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAg > 1:1358619/1‑52 (MQ=255) aCCTTACGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGa > 1:5053798/1‑67 (MQ=255) cTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCttt < 1:1788979/40‑1 (MQ=255) cTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCttt < 1:1139219/40‑1 (MQ=255) ttGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATg < 1:4801406/66‑1 (MQ=255) tGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTcaca > 1:1618097/1‑60 (MQ=255) gcgcCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCa > 1:1298649/1‑66 (MQ=255) gcgcCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCa > 1:2600754/1‑66 (MQ=255) gcgcCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCa > 1:3035841/1‑66 (MQ=255) | TCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCA > W3110S.gb/1157763‑1157896 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |