| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,224,919 | G→A | 34.7% | intergenic (‑423/‑123) | ymgG ← / → ymgH | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,224,919 | 0 | G | A | 34.7% | 87.6 / 72.4 | 75 | intergenic (‑423/‑123) | ymgG/ymgH | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (29/20); new base A (9/17); total (38/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.42e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCATCAGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTAAAAAAT > W3110S.gb/1224855‑1224988 | ttCATCAGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGa < 1:4930478/70‑1 (MQ=255)ttCATCAGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGa < 1:1263101/70‑1 (MQ=255) atcaGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAg > 1:3175784/1‑70 (MQ=255) atcaGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGt < 1:1715376/71‑1 (MQ=255) caGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTa > 1:2250454/1‑62 (MQ=255) caGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTa > 1:1506315/1‑62 (MQ=255) aGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAg > 1:1544515/1‑67 (MQ=255) aGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGtat < 1:218442/70‑1 (MQ=255) aGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGtat > 1:4568988/1‑70 (MQ=255) aGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGtat < 1:2638084/70‑1 (MQ=255) aGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGtat > 1:3176074/1‑70 (MQ=255) aGCAACGATTACATTAGTCATTTTATTTTGCCGACAGCCTCATTGTCGAAAGATAAGCGTACGACAGtat < 1:316071/70‑1 (MQ=255) gCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGtatta < 1:3049876/71‑1 (MQ=255) gCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGtatta < 1:2454715/71‑1 (MQ=255) aCGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTa > 1:719837/1‑57 (MQ=255) aCGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTa > 1:3482500/1‑57 (MQ=255) cGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGtattat > 1:3784145/1‑68 (MQ=255) cGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGtattat > 1:3144627/1‑68 (MQ=255) ttACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtat < 1:4181094/62‑1 (MQ=255) ttACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAg > 1:2196734/1‑68 (MQ=255) ttACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaa < 1:3491279/71‑1 (MQ=255) ttACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaa > 1:4147203/1‑70 (MQ=255) ttACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaa > 1:4284174/1‑70 (MQ=255) tACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGaa > 1:4296597/1‑69 (MQ=255) tACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaa > 1:2196/1‑69 (MQ=255) tACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaa > 1:1255447/1‑69 (MQ=255) tAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGt > 1:831736/1‑70 (MQ=255) tCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGta < 1:2244317/52‑1 (MQ=255) tCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGAtt < 1:523411/71‑1 (MQ=255) attttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGaaaa > 1:1101383/1‑61 (MQ=255) attttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGaaaa > 1:3265486/1‑61 (MQ=255) attttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGAtttt > 1:2331483/1‑71 (MQ=255) attttattttGCCGACGCCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGAttt < 1:2154732/70‑1 (MQ=255) ttttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGaa < 1:1327830/58‑1 (MQ=255) tttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGAttttt > 1:2084839/1‑70 (MQ=255) ttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTa > 1:703546/1‑71 (MQ=255) ttattttGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa > 1:3704006/1‑58 (MQ=255) tattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTa > 1:2600716/1‑70 (MQ=255) tattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTAt > 1:904910/1‑71 (MQ=255) tattttCCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTAt > 1:1862/1‑71 (MQ=255) ccGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTTATTTTTTATcc < 1:1222095/66‑1 (MQ=38) ccGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATcc < 1:3157617/66‑1 (MQ=255) gACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCaa > 1:1180776/1‑66 (MQ=255) aCGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCaa > 1:1448035/1‑65 (MQ=255) gCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTc < 1:187100/71‑1 (MQ=255) tCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTaca < 1:1149571/64‑1 (MQ=255) tCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaa < 1:2517534/39‑1 (MQ=255) tCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGAtt < 1:1765857/49‑1 (MQ=255) cATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAgcg > 1:666585/1‑71 (MQ=255) cATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACtt < 1:465344/66‑1 (MQ=255) cATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACtt < 1:3729575/66‑1 (MQ=255) ttGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTAt > 1:2977909/1‑52 (MQ=255) ttGTCGAAAGATAAGCATACGACAGTATTATCAGaa < 1:869308/36‑1 (MQ=255) ttGTCGAAAGATAAGCATACGACAGTATTATCAGaa < 1:1122086/36‑1 (MQ=255) gTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTc > 1:4392336/1‑63 (MQ=255) gTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACt > 1:2800913/1‑71 (MQ=255) gTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACt > 1:4750343/1‑71 (MQ=255) gTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACt > 1:4441965/1‑71 (MQ=255) tCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTg < 1:4912178/71‑1 (MQ=255) gAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGAtt < 1:4211685/41‑1 (MQ=255) gAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGAtt < 1:4036897/41‑1 (MQ=255) gAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCg < 1:2199970/71‑1 (MQ=255) aaGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCgtg > 1:1795396/1‑71 (MQ=255) aGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCgtg > 1:3227011/1‑70 (MQ=255) aGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTa > 1:914674/1‑43 (MQ=255) aaGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTaaa > 1:3151692/1‑70 (MQ=255) aGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTaaaaa < 1:4121540/71‑1 (MQ=255) aGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTaaaaa < 1:1933263/71‑1 (MQ=255) aGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTaaaa < 1:4946418/70‑1 (MQ=255) aGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTaaaa < 1:3340380/70‑1 (MQ=255) aGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTaaaa < 1:1728038/70‑1 (MQ=255) aGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTaaaa < 1:105215/70‑1 (MQ=255) aGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTaaaa < 1:4772033/70‑1 (MQ=255) cGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTAAAAAAt < 1:4679632/71‑1 (MQ=255) cGTACGACAGTATTATAAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTAAAAAAt < 1:3152453/71‑1 (MQ=255) | TTCATCAGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTAAAAAAT > W3110S.gb/1224855‑1224988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |