| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,234,526 | T→C | 19.1% | T28A (ACG→GCG) | dsbB ← | oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,234,526 | 0 | T | C | 19.1% | 100.2 / 20.4 | 47 | T28A (ACG→GCG) | dsbB | oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I |
| Reads supporting (aligned to +/- strand): ref base T (21/17); new base C (6/3); total (27/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01 | |||||||||||
ACGCGCAGCGTTCATAAATACAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGAACAT > W3110S.gb/1234457‑1234587 | aCGCGCAGCGTTCATAAATACAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTc < 1:3245820/71‑1 (MQ=255) cgcAGCGTTCATAAATACAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTc < 1:270278/68‑1 (MQ=255) cgcAGCGTTCATAAATACAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTc < 1:264196/68‑1 (MQ=255) cAGCGTTCATAAATACAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTc < 1:3593372/71‑1 (MQ=255) gTTCATAAATACAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGt > 1:858988/1‑71 (MQ=255) aTAAATACAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCa > 1:1380600/1‑59 (MQ=255) aaaTACAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGt < 1:929932/65‑1 (MQ=255) cAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGt < 1:3229437/71‑1 (MQ=255) cAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGt < 1:1566813/71‑1 (MQ=255) agagCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTa < 1:3088359/71‑1 (MQ=255) cACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACg > 1:1091464/1‑71 (MQ=255) cGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGCCAGTTCCAGTGCCAGAGCAGTAAACGc > 1:4677647/1‑70 (MQ=255) aaGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGCCAGTTCCAGTGCCAGAGCAGTAAACGCCATc < 1:4738394/71‑1 (MQ=255) aaGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGCCAGTTCCAGTGCCAGAGCAGTAAACGCCATc < 1:4737814/71‑1 (MQ=255) aGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATc > 1:4818023/1‑70 (MQ=255) ggTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCaa > 1:1617288/1‑71 (MQ=255) ggTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCaa > 1:3827136/1‑71 (MQ=255) gTTTCAGTAACATCACATGCTGGAACCACAGCGCCGCCAGTTCCAGTGCCAGAGCAGTaa > 1:1430598/1‑60 (MQ=255) tttCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACa < 1:3996088/71‑1 (MQ=255) ttCAGTAACATCACATGCTGGAACCACAGCGCCGCCAGTTCCAGTGCCAGAGCAGTa < 1:100281/57‑1 (MQ=255) aaCATCACATGCTGGAACCACAGCGCCGCCAGTTCCAGTGCCAGAGCAGTaaa > 1:30600/1‑53 (MQ=255) tCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGccc > 1:3186209/1‑71 (MQ=255) tCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGccc > 1:4561736/1‑71 (MQ=255) tCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCAT‑AACAGCCACGCGcccc > 1:4648613/1‑71 (MQ=255) acaTGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCa > 1:1169389/1‑62 (MQ=255) acaTGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCg > 1:4306991/1‑71 (MQ=255) tGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTa < 1:752101/42‑1 (MQ=255) tGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGcc < 1:3198423/71‑1 (MQ=255) tGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGcc < 1:4896957/71‑1 (MQ=255) gCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCt > 1:5033711/1‑71 (MQ=255) gCTGGAACCACAGCGCCGCCTGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCt > 1:2758001/1‑71 (MQ=255) gCTGGAACCACAGCGCCGCCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCt > 1:1187675/1‑71 (MQ=255) gCTGGAACCACAGCGCCGCCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCt > 1:5005557/1‑71 (MQ=255) cTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTaaa > 1:1087280/1‑42 (MQ=255) cTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTaaa > 1:4605975/1‑42 (MQ=255) tGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACTCCATCAACAGCCACGCGCCCCGGCCt < 1:2508341/69‑1 (MQ=255) tGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCt < 1:3736456/69‑1 (MQ=255) tGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCt < 1:3636711/69‑1 (MQ=255) ggAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATc < 1:2946194/47‑1 (MQ=255) gAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTtgtg < 1:686516/71‑1 (MQ=255) gAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTtg > 1:2343013/1‑69 (MQ=255) gAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTtg > 1:2020500/1‑69 (MQ=255) aaCCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCaa > 1:606930/1‑47 (MQ=255) acaGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGaa > 1:753903/1‑68 (MQ=255) acaGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGAACAt > 1:2042176/1‑71 (MQ=255) caGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGcc > 1:122744/1‑54 (MQ=255) caGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGAACAt > 1:3536106/1‑70 (MQ=255) tCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCAcg < 1:4354522/42‑1 (MQ=255) tCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCAcg < 1:1912415/42‑1 (MQ=255) tCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCAcg < 1:1889001/42‑1 (MQ=255) tCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCAcg < 1:1878546/42‑1 (MQ=255) | ACGCGCAGCGTTCATAAATACAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGAACAT > W3110S.gb/1234457‑1234587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |